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25384 Fecal Bacteria-only Pathogen Panel, NAT

Fecal Bacteria-only Pathogen Panel, NAT
Test Code: SSCSNAT
Synonyms/Keywords
​Salmonella, Shigella, Campylobacter, Escherichia coli, O157, H7, STEC, EHEC Shiga Toxin, Stool, Feces, Nucleic Acid Test, Enteric Pathogen
Test Components
​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.
Useful For
Detection of Salmonella, Shigella, Campylobacter (C. jejuni and C. coli species only) and Shiga Toxin-producing Escherichia coli (STEC, otherwise known as enterohemorrhagic E. coli or EHEC) serotype O157:H7 and others, are routinely reported.  Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens.  Consider a Clostridium difficile toxin assay for C. difficile-associated nosocomial diarrhea instead of stool nucleic acid test.  (See Clostridium difficile Toxin Assay).
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Feces ​ParaPak C & S
​Cary Blair,
ParaPak C & S
​1 gram ​1 gram ​1 gram
Collection Processing Instructions

NOTE - the preferred specimen container is Cary Blair transport. Submitting in another type of sterile container without preservative is not desirable and may delay testing.
Remove cap and place approximately 1 gram of the raw stool into the transport medium or a sufficient amount to bring the liquid level up to the "fill to here" line.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Acceptable Specimen Types

​Feces, Liquid or Soft Raw Stool (preferably in Cary Blair transport) 

Specimen Stability Information
Specimen Type Temperature Time
​Feces ​ ​Room Temperature ​ 24 hours
​Refrigerated 1​20 hours​
​ParaPak C & S (Cary Blair) filled to the fill line ​Refrigerated (preferred) ​120 hours
Rejection Criteria
Rectal Swabs
Feces at room temperature equal to or greater than 24 hours old
Feces refrigerated equal to or greater than 120 hours old
ParaPak C & S equal to or greater than 120 hours old​
​Frozen feces
Interference
​Any inhibition of amplification will be detected through the use of an internal amplification control.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Sunday through Friday
6-8 hours*
​Nucleic Acid Test
*Testing performed once/day.
Sample must be received in the lab by 0400 to be tested same day. ​ ​ ​ ​
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative for Salmonella, Shigella, Campylobacter (C. jejuni and C. coli),
and Shiga Toxin-producing E. coli (Shiga Toxin 1 & 2)
Interpretation
​Antibiotic susceptibility testing is not normally performed, but may be requested on Salmonella and Shigella isolates through the Wisconsin State Laboratory of Hygiene at an additional charge.  Antimicrobial therapy is not recommended for E. coli STEC infections, as it may enhance Shiga toxin release and increase the risk of hemolytic uremic syndrome (HUS).
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87505
Synonyms/Keywords
​Salmonella, Shigella, Campylobacter, Escherichia coli, O157, H7, STEC, EHEC Shiga Toxin, Stool, Feces, Nucleic Acid Test, Enteric Pathogen
Test Components
​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.
Ordering Applications
Ordering Application Description
​​COM ​Fecal Bacterial Pathogens, NAT
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Feces ​ParaPak C & S
​Cary Blair,
ParaPak C & S
​1 gram ​1 gram ​1 gram
Collection Processing

NOTE - the preferred specimen container is Cary Blair transport. Submitting in another type of sterile container without preservative is not desirable and may delay testing.
Remove cap and place approximately 1 gram of the raw stool into the transport medium or a sufficient amount to bring the liquid level up to the "fill to here" line.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Acceptable Specimen Types

​Feces, Liquid or Soft Raw Stool (preferably in Cary Blair transport) 

Specimen Stability Information
Specimen Type Temperature Time
​Feces ​ ​Room Temperature ​ 24 hours
​Refrigerated 1​20 hours​
​ParaPak C & S (Cary Blair) filled to the fill line ​Refrigerated (preferred) ​120 hours
Rejection Criteria
Rectal Swabs
Feces at room temperature equal to or greater than 24 hours old
Feces refrigerated equal to or greater than 120 hours old
ParaPak C & S equal to or greater than 120 hours old​
​Frozen feces
Interference
​Any inhibition of amplification will be detected through the use of an internal amplification control.
Useful For
Detection of Salmonella, Shigella, Campylobacter (C. jejuni and C. coli species only) and Shiga Toxin-producing Escherichia coli (STEC, otherwise known as enterohemorrhagic E. coli or EHEC) serotype O157:H7 and others, are routinely reported.  Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens.  Consider a Clostridium difficile toxin assay for C. difficile-associated nosocomial diarrhea instead of stool nucleic acid test.  (See Clostridium difficile Toxin Assay).
Test Components
​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.
Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative for Salmonella, Shigella, Campylobacter (C. jejuni and C. coli),
and Shiga Toxin-producing E. coli (Shiga Toxin 1 & 2)
Interpretation
​Antibiotic susceptibility testing is not normally performed, but may be requested on Salmonella and Shigella isolates through the Wisconsin State Laboratory of Hygiene at an additional charge.  Antimicrobial therapy is not recommended for E. coli STEC infections, as it may enhance Shiga toxin release and increase the risk of hemolytic uremic syndrome (HUS).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Sunday through Friday
6-8 hours*
​Nucleic Acid Test
*Testing performed once/day.
Sample must be received in the lab by 0400 to be tested same day. ​ ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87505
For most current information refer to the Marshfield Laboratory online reference manual.