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22510 Culture, Fungus-General

Culture, Fungus-General
Test Code: FUNG
Synonyms/Keywords
​​Fungus culture, Candida culture, Culture for yeast
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​
Any
(except blood and bone marrow)​
Sterile, leakproof container
(e.g. Sage container)​
 ESwab™ or other Amies medium based swab

1-10 mL​
0.5 mL fluids and secretions
 ​
1 ESwab™ or 1 Amies medium based swab
​
0.5 g tissues
​
one swab
(for oral or urogenital sources only)​​
Collection Processing Instructions
Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.  Do not freeze specimens at -20°C, or in a frost-free freezer.
 
Specimens should be collected in sterile leakproof containers without preservatives.  Moisten small pieces of tissue with several drops of sterile, non-bacteriostatic normal saline.  Swab-collected specimens and fecal specimens are not recommended. One swab in Amies or Stuarts transport medium may be submitted from oral or urogenital sources: swabs from other sites are not recommended.  Note: Swab specimens submitted for fungal culture will not include fungal smear direct exam.

Swabs:
- ESwab: one swab can be collected for Gram stain, aerobic, anaerobic and  fungal cultures.
- Other Amies medium swab types: collect one swab for every culture and stain to be ordered.
 
 
For nail and skin samples, see Culture, Fungus-Dermal (FSKIN).
 
For blood and bone marrow fungal culture, see Fungal Culture, Blood (MISC).

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Acceptable Specimen Types
​Bone, Bronchial, Catheter Tip, Fine Needle Aspirate, Fluid, Hardware, Respiratory Secretions, Swab, Synovial, Tissue, or Urine.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Room Temperature​
 <72 hours​
​Swabs (single, dual) in preservative media (Amies)​
​Room Temperature
​<= 4 hours
​Refrigerate​
​
< 24 hours
​
ESwab
​
​Room Temperature
​<= 48 hours
​Refrigerate
​<= 48 hours
All other specimen types​ Room Temperature <1 hour
​Refrigerate​
 <72 hours​
Rejection Criteria
Dried swabs or tissues
Specimens submitted in formalin or other preservatives​
​Tissues in large volumes of saline
​Swab specimens collected in preservative​ media (Amies) >24 hours
​ESwab specimens >48 hours
​Specimens older than 3 days
​Leaking specimens
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​ ​

Conventional culture, microscopic exam with fungal-specific stain, manual or automated identification methods and nucleic acid sequence analysis as needed.​​

Test Information

​The fungal load in a clinical specimen may be low​. Specimens with less than the recommended volumes may therefore lead to a false negative result.

Due to high incidence of blastomycosis in this region, a direct fungal exam is performed and billed on all lower respiratory tract and sinus specimens, and on pleural fluids that are submitted for fungal culture with the exception of swab specimens. 

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Range Information
Performing Location Reference Range
All Performing Sites

No fungal elements observed (fungal smear)

Culture negative for Fungi​

See Critical Value List for list of current critical values.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87102​ ​ ​ Culture, other source (except blood)​ ​
87106​ ​ ​ (Yeast) Organism Identification​ ​​As needed
87107​ ​ ​ (Mold) Organism Identification​ ​​As needed
87206​ ​ ​ Direct microscopy, respiratory specimens ​As needed
87153​ ​ ​ Identification by nucleic acid sequencing method ​​As needed
​87186
​​
​
​​MIC susceptibility, micro-broth dilution
​As needed
Synonyms/Keywords
​​Fungus culture, Candida culture, Culture for yeast
Ordering Applications
Ordering Application Description
​Centricity ​Cult. Fungus-General
​Cerner ​Fungus Culture
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​
Any
(except blood and bone marrow)​
Sterile, leakproof container
(e.g. Sage container)​
 ESwab™ or other Amies medium based swab

1-10 mL​
0.5 mL fluids and secretions
 ​
1 ESwab™ or 1 Amies medium based swab
​
0.5 g tissues
​
one swab
(for oral or urogenital sources only)​​
Collection Processing
Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.  Do not freeze specimens at -20°C, or in a frost-free freezer.
 
Specimens should be collected in sterile leakproof containers without preservatives.  Moisten small pieces of tissue with several drops of sterile, non-bacteriostatic normal saline.  Swab-collected specimens and fecal specimens are not recommended. One swab in Amies or Stuarts transport medium may be submitted from oral or urogenital sources: swabs from other sites are not recommended.  Note: Swab specimens submitted for fungal culture will not include fungal smear direct exam.

Swabs:
- ESwab: one swab can be collected for Gram stain, aerobic, anaerobic and  fungal cultures.
- Other Amies medium swab types: collect one swab for every culture and stain to be ordered.
 
 
For nail and skin samples, see Culture, Fungus-Dermal (FSKIN).
 
For blood and bone marrow fungal culture, see Fungal Culture, Blood (MISC).

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Acceptable Specimen Types
​Bone, Bronchial, Catheter Tip, Fine Needle Aspirate, Fluid, Hardware, Respiratory Secretions, Swab, Synovial, Tissue, or Urine.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Room Temperature​
 <72 hours​
​Swabs (single, dual) in preservative media (Amies)​
​Room Temperature
​<= 4 hours
​Refrigerate​
​
< 24 hours
​
ESwab
​
​Room Temperature
​<= 48 hours
​Refrigerate
​<= 48 hours
All other specimen types​ Room Temperature <1 hour
​Refrigerate​
 <72 hours​
Rejection Criteria
Dried swabs or tissues
Specimens submitted in formalin or other preservatives​
​Tissues in large volumes of saline
​Swab specimens collected in preservative​ media (Amies) >24 hours
​ESwab specimens >48 hours
​Specimens older than 3 days
​Leaking specimens
Reference Range Information
Performing Location Reference Range
All Performing Sites

No fungal elements observed (fungal smear)

Culture negative for Fungi​

See Critical Value List for list of current critical values.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​ ​

Conventional culture, microscopic exam with fungal-specific stain, manual or automated identification methods and nucleic acid sequence analysis as needed.​​

For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87102​ ​ ​ Culture, other source (except blood)​ ​
87106​ ​ ​ (Yeast) Organism Identification​ ​​As needed
87107​ ​ ​ (Mold) Organism Identification​ ​​As needed
87206​ ​ ​ Direct microscopy, respiratory specimens ​As needed
87153​ ​ ​ Identification by nucleic acid sequencing method ​​As needed
​87186
​​
​
​​MIC susceptibility, micro-broth dilution
​As needed
For most current information refer to the Marshfield Laboratory online reference manual.