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22359 Culture, Urine-With Susceptibilities

Culture, Urine-With Susceptibilities
Test Code: URNS
Synonyms/Keywords
Urine C and S, Urine Culture
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Urine, clean catch midstream, first morning specimen preferred​ ​ Sterile, leakproof container​ ​  VACUETTE® urine transport tube, No Additive 1  - 6.5 mL 1 mL ​ ​
Collection Processing Instructions
Specimens should be delivered to the microbiology laboratory as soon as possible. Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.
 
Suprapubic aspirates are submitted in the collection syringe WITHOUT NEEDLE or a sterile screw-capped container.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​Urine, Unpreserved Refrigerate <=72 hours​
Urine, Sterile Container​ Refrigerate <=72 hours​
Rejection Criteria
​Urine in swab or Eswab
​Urine >72 Hrs
​Leaking containers
​Non sterile containers (Hats)
​Unidentified specimen
​​Urine in preservative
Unrefrigerated urine​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 2 days​ Quantitative Culture (0.001 mL)​
Test Information
Method is sensitive to 1,000 organisms/mL of urine. Voided and cath cultures containing 3 or more species are considered contaminated unless one organism is predominant. Gram stains are not routinely performed on urine submitted for culture; request separately, if desired. A colony count is reported.
 
Antimicrobial Susceptibility Testing (AST) will be performed on significant bacterial isolates with unpredictable patterns if standardized methods are available based upon Clinical Laboratory Standards Institute guidelines.  Methods used include disk diffusion, minimum inhibitory concentration (MIC) by broth microdilution, or MIC by Etest®.
 
A listing of the tested and reported antimicrobial agents can be seen at Antimicrobial Susceptibility Panels. If testing against a specific antimicrobial agent is desired, contact the microbiology laboratory.
 
The microbiology laboratory uses selective reporting of susceptibility results in order to promote rational antimicrobial usage. For more information on selective reporting, please contact the microbiology technical manager. 
 
Standardized AST is not available for all bacterial species, in which case an MIC determined by a non-standardized method and without interpretation may be available upon request. Contact the microbiology laboratory for more information.
 
'Eswab' is a trademark of Copan Diagnostics Inc.  'Etest' is a registered trademark of bioMerieux Inc.  'VACUETTE' is a registered trademark of Greiner Bio One International GmbH. ​ ​ ​ ​
Reference Range Information
Performing Location Reference Range
All Performing Sites No Growth (<1,000 CFU/mL)​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87086​ Colony Count​
87088​ Isolation and Presumptive Identification​ As needed​
870​77​​​ ​Definitive Identification​ ​As needed
87186​ MIC susceptibility, micro-broth dilution As needed​
87184​ KB (disk), susceptibility As needed​
87181 ​MIC susceptibility, E-test®, gradient strip ​As needed
​87147 ​Serotyping/grouping ​As needed
Synonyms/Keywords
Urine C and S, Urine Culture
Ordering Applications
Ordering Application Description
​Centricity ​Cult. Urn-Id & Sensi
​Cerner ​Culture, Urine-with Susceptibilities
​COM ​Culture, Urine-Id Org & Sensi
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Urine, clean catch midstream, first morning specimen preferred​ ​ Sterile, leakproof container​ ​  VACUETTE® urine transport tube, No Additive 1  - 6.5 mL 1 mL ​ ​
Collection Processing
Specimens should be delivered to the microbiology laboratory as soon as possible. Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.
 
Suprapubic aspirates are submitted in the collection syringe WITHOUT NEEDLE or a sterile screw-capped container.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​Urine, Unpreserved Refrigerate <=72 hours​
Urine, Sterile Container​ Refrigerate <=72 hours​
Rejection Criteria
​Urine in swab or Eswab
​Urine >72 Hrs
​Leaking containers
​Non sterile containers (Hats)
​Unidentified specimen
​​Urine in preservative
Unrefrigerated urine​
Reference Range Information
Performing Location Reference Range
All Performing Sites No Growth (<1,000 CFU/mL)​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 2 days​ Quantitative Culture (0.001 mL)​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87086​ Colony Count​
87088​ Isolation and Presumptive Identification​ As needed​
870​77​​​ ​Definitive Identification​ ​As needed
87186​ MIC susceptibility, micro-broth dilution As needed​
87184​ KB (disk), susceptibility As needed​
87181 ​MIC susceptibility, E-test®, gradient strip ​As needed
​87147 ​Serotyping/grouping ​As needed
For most current information refer to the Marshfield Laboratory online reference manual.