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22320 Antinuclear Antibody Immunofluorescent (ANA-HEp2)

Antinuclear Antibody Immunofluorescent (ANA-HEp2)
Test Code: ANA
Synonyms/Keywords
ANA, FANA, IFA-ANA​
Useful For
Antinuclear antibody (ANA) is a general term used to describe autoantibodies against various cell nuclear proteins. Antibodies specific to DNA show a high disease correlation with systemic lupus erythematosus (SLE). A number of other nuclear and cytoplasmic macromolecules have been detected, and are associated with other connective tissue diseases. Because a number of these antibodies appear to be of diagnostic and/or prognostic use in progressive systemic sclerosis, mixed connective tissue disease, Sjögren's syndrome, polymyositis, and rheumatoid arthritis, ANA testing is no longer considered only a test to rule out SLE. ANA testing is now recognized as a general screening tool for connective tissue disease. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
Test Information
This test is performed using Hep-2 substrate. Positives are tittered at an additional charge, and pattern is reported. The following patterns will be reported when observed: Homogenous, Nucleolar, Speckled, Centromere, Cytplasmic, Dense Fine Speckled, Proliferating Cell Nuclear Antigen, Nuclear Membrane, Multiple Nuclear Dot, and "Other". An interpretive comment will be added when appropriate. See the Mixed Connective Tissue Disease Panel Algorithm for suggested follow-up testing.
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative: <1:80 ​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86038​ 1​ Screen​
86039​ 1​ Titer, if positive​
Synonyms/Keywords
ANA, FANA, IFA-ANA​
Ordering Applications
Ordering Application Description
​Centricity ​ANA Immunofluorescent (HEp2)
​Cerner ​ANA Immunofluorescent (HEp2)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Useful For
Antinuclear antibody (ANA) is a general term used to describe autoantibodies against various cell nuclear proteins. Antibodies specific to DNA show a high disease correlation with systemic lupus erythematosus (SLE). A number of other nuclear and cytoplasmic macromolecules have been detected, and are associated with other connective tissue diseases. Because a number of these antibodies appear to be of diagnostic and/or prognostic use in progressive systemic sclerosis, mixed connective tissue disease, Sjögren's syndrome, polymyositis, and rheumatoid arthritis, ANA testing is no longer considered only a test to rule out SLE. ANA testing is now recognized as a general screening tool for connective tissue disease. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative: <1:80 ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86038​ 1​ Screen​
86039​ 1​ Titer, if positive​
For most current information refer to the Marshfield Laboratory online reference manual.