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26839 Granulocyte Antibody Screen, Serum (LAGGN)

Granulocyte Antibody Screen, Serum (LAGGN)
Test Code: LAGGNSO
Synonyms/Keywords

Anti-Leukocyte Ab, Anti-Leukocyte Antibodies, Anti-Neutrophil, Antigranulocyte Antibodies, Granulocyte Ab, Granulocyte Binding IgG, LAGGN, LAGGT, Leukoagglutinin, Neutrophil Antibodies​​

Test Components

​GIFT/GAT Interpretation

GIFT Result

GAT Result

Useful For
 Work-up of individuals with autoimmune neutropenia

 Work-up of individuals having febrile nonhemolytic transfusion reactions

 Work-up for alloimmune neonatal neutropenia

 This test is not useful for the diagnosis of neutropenia due to marrow suppression by drugs or tumors.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Serum
​Red Top Tube (RTT)

​1.5 mL
​0.3 mL
Collection Processing Instructions

*Only a specimen collected before a transfusion reaction is acceptable.

​Serum Separator Tube (SST) are NOT acceptable.

Centrifuge and aliquot serum into a plastic vial.

Specimen Stability Information
Specimen TypeTemperatureTime`



​​​Serum​



​Refrigerated (preferred)
​30 days
​Ambient
​7 days
Frozen
​365 days
Rejection Criteria

Gross Hemolysis

Interference

​Testing for patients who receive human monoclonal antibodies like daratumumab may cause false-positive results due to interfering substances. Hemolytic samples have the potential for causing false-positivity and will, therefore, be rejected.​

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport Available
Methodology/Instrumentation

Mayo Clinic Laboratories​
​​Tuesday, Thursday
​7 to 15 days
GIFT Result:  ​Flow Cytometry
GAT Result:  Agglutination​​ Test
Reference Lab
Test Information

​Granulocyte antibodies are induced by pregnancy, prior transfusion, or transplants. These antibodies can cause neutropenia in various autoimmune disorders. Febrile nonhemolytic transfusion reactions and alloimmune neonatal neutropenia may also be caused by granulocyte associated antibodies, including anti-human leukocyte antigen antibodies.

Reference Range Information

Not applicable

Interpretation

A positive result can be due to anti-granulocyte antibodies and anti-human leukocyte antigen antibodies.

This test cannot distinguish between allo- or autoantibodies, nor can it determine the specificity of the detected antibody. Results should be correlated to clinical history.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments

​86021
​2
Synonyms/Keywords

Anti-Leukocyte Ab, Anti-Leukocyte Antibodies, Anti-Neutrophil, Antigranulocyte Antibodies, Granulocyte Ab, Granulocyte Binding IgG, LAGGN, LAGGT, Leukoagglutinin, Neutrophil Antibodies​​

Test Components

​GIFT/GAT Interpretation

GIFT Result

GAT Result

Ordering Applications
Ordering ApplicationDescription

​Cerner
​Granulocyte Antibody Screen, Serum (LAGGN)​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Serum
​Red Top Tube (RTT)

​1.5 mL
​0.3 mL
Collection Processing

*Only a specimen collected before a transfusion reaction is acceptable.

​Serum Separator Tube (SST) are NOT acceptable.

Centrifuge and aliquot serum into a plastic vial.

Specimen Stability Information
Specimen TypeTemperatureTime`



​​​Serum​



​Refrigerated (preferred)
​30 days
​Ambient
​7 days
Frozen
​365 days
Rejection Criteria

Gross Hemolysis

Interference

​Testing for patients who receive human monoclonal antibodies like daratumumab may cause false-positive results due to interfering substances. Hemolytic samples have the potential for causing false-positivity and will, therefore, be rejected.​

Useful For
 Work-up of individuals with autoimmune neutropenia

 Work-up of individuals having febrile nonhemolytic transfusion reactions

 Work-up for alloimmune neonatal neutropenia

 This test is not useful for the diagnosis of neutropenia due to marrow suppression by drugs or tumors.
Test Components

​GIFT/GAT Interpretation

GIFT Result

GAT Result

Reference Range Information

Not applicable

Interpretation

A positive result can be due to anti-granulocyte antibodies and anti-human leukocyte antigen antibodies.

This test cannot distinguish between allo- or autoantibodies, nor can it determine the specificity of the detected antibody. Results should be correlated to clinical history.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport Available
Methodology/Instrumentation

Mayo Clinic Laboratories​
​​Tuesday, Thursday
​7 to 15 days
GIFT Result:  ​Flow Cytometry
GAT Result:  Agglutination​​ Test
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments

​86021
​2
For most current information refer to the Marshfield Laboratory online reference manual.