Measles, Nucleic Acid Test

Marshfield Lab Public WebSite

Marshfield Clinic Public WebSite

Test Code: MEASLESNAT

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

Measles, Measles PCR

Test Components

Real-Time Polymerase Chain Reaction

Useful For

Detection of Measles virus

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Nasopharyngeal Swab (Preferred)	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA
Oropharyngeal (throat) Swab	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA
Combined NP swab and throat swab	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA

Collection Processing Instructions

· Detection of Measles RNA is most successful when samples are collected on the first day of rash through the 3 days following onset of rash.
· Detection of measles RNA by RT-PCR may be successful as late as 10-14 days after rash onset.
· Collect samples as soon as measles disease is suspected.

Acceptable Body Sites

Acceptable Specimen Types

Specimen Type(s): NP Swab, Throat Swab

Specimen Stability Information

Specimen Type	Temperature	Time
Swab in UTM	Refrigerated	<=72 hours
	Frozen	3 months

Rejection Criteria

Calcium alginate swabs Cotton swabs Wooden shaft swabs Dry swabs Swabs in unacceptable transport media Improper specimen type (e.g. Nasal swab, buccal swab, etc.) Specimens outside of the stability range.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Marshfield	Monday - Sunday	3hrs	Polymerase Chain Reaction
Note testing is performed once per day unless emergent infection prevention situation is present. Contact the Microbiology/Molecular Pathology Lab if necessary.

Reference Range Information

Performing Location	Reference Range
Marshfield Laboratories	Measles PCR: Negative

Interpretation

Technical Contact:
Dr. Sophie Arbefeville and/or Dr. Taylor Wahlig

Interpretive Contact:
Dr. Sophie Arbefeville and/or Dr. Taylor Wahlig

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
87798		1

Synonyms/Keywords

Measles, Measles PCR

Test Components

Real-Time Polymerase Chain Reaction

Ordering Applications

Ordering Application	Description

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Nasopharyngeal Swab (Preferred)	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA
Oropharyngeal (throat) Swab	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA
Combined NP swab and throat swab	Universal Transport Media (UTM)	Viral Transport Media (VTM)	1 swab, in 3 mL	1 swab, in 3 mL	NA

Collection Processing

Acceptable Body Sites

Acceptable Specimen Types

Specimen Type(s): NP Swab, Throat Swab

Specimen Stability Information

Specimen Type	Temperature	Time
Swab in UTM	Refrigerated	<=72 hours
	Frozen	3 months

Rejection Criteria

Calcium alginate swabs Cotton swabs Wooden shaft swabs Dry swabs Swabs in unacceptable transport media Improper specimen type (e.g. Nasal swab, buccal swab, etc.) Specimens outside of the stability range.

Useful For

Detection of Measles virus

Test Components

Real-Time Polymerase Chain Reaction

Reference Range Information

Performing Location	Reference Range
Marshfield Laboratories	Measles PCR: Negative

Interpretation

Technical Contact:
Dr. Sophie Arbefeville and/or Dr. Taylor Wahlig

Interpretive Contact:
Dr. Sophie Arbefeville and/or Dr. Taylor Wahlig

For more information visit:

http://labtestsonline.org

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Marshfield	Monday - Sunday	3hrs	Polymerase Chain Reaction
Note testing is performed once per day unless emergent infection prevention situation is present. Contact the Microbiology/Molecular Pathology Lab if necessary.

For billing questions, see Contacts

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
87798		1

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For most current information refer to the Marshfield Laboratory online reference manual.

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26826 Measles, Nucleic Acid Test