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26515 Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies (EBVPV)

Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies (EBVPV)
Test Code: MISC
Synonyms/Keywords


EBV (Epstein-Barr Virus)

EBV Detection by PCR (Polymerase Chain Reaction)

EBV Detection by PCR (Polymerase Chain Reaction), Spinal Fluid

EBV Detection by Real-Time PCR

Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction

Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction), CSF

Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR)

Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR), CSF

Epstein-Barr Virus Detection by Real-Time PCR

Infectious Mononucleosis

LightCycler EBV

PCR (Polymerase Chain Reaction)

PCR, Epstein-Barr​


Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens

Diagnosis of disease due to EBV

This test should not be used to screen asymptomatic patients.



Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

Submit only 1 of the following specimens: ​ ​​ ​

Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis), amniotic, or ocular.

Collection Instructions: Do not centrifuge.​


​Sterile screwcap 5-mL plastic vial
Sterile container
​0.5 mL
​0.3 mL


Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate


​Sterile screwcap 5-mL plastic vial
Sterile container
​1.5 mL​
​1.0 mL


Swab

Sources: Eye and upper respiratory (nasal, throat).

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)


​Multimicrobe media (M4-RT) and Eswabs



Bone marrow
Additional Information: Clotted specimens will be rejected.
​EDTA Lavender TopTube (LTT)

​0.5 mL

Tissue

Sources: Brain, colon, kidney, liver, lung, cornea, etc.

Collection Instructions: Submit only fresh tissue.​


​Multimicrobe medium (M4-RT)
​Sterile container containing 1-2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)
Entire collection
2 x 2-mm biopsy
Collection Processing Instructions

Specimen source is required.

Specimen Stability Information
Specimen TypeTemperature

Time
Varies​

​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Formalin-fixed and paraffin-embedded tissues
Heat-inactivated specimens
Clotted EDTA blood



Interference

A negative result does not eliminate the possibility of Epstein-Barr virus (EBV) infection of the central nervous system.

This assay may detect viremia or viral shedding in asymptomatic individuals. However, this assay is only to be used for patients with a clinical history and symptoms consistent with EBV infection and must be interpreted in the context of the clinical picture.



Performing Laboratory Information
Performing LocationDay(s) Test Performed
Analytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories
Monday through Friday
​Same day/1 to 4 days​
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
Test Information

​Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis, Burkitt lymphoma, and in Southern China, nasopharyngeal carcinoma. EBV-associated central nervous system (CNS) disease is most frequently associated with primary CNS lymphoma in patients with AIDS. In addition, CNS infection associated with the detection of EBV DNA can be seen in immunocompetent patients.

Reference Range Information
Performing Location
Reference Range
Mayo Clinic Laboratories​

Negative

Reference values apply to all ages.​


Interpretation

​Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus. EBV DNA is not detected in CSF from patients without CNS disease caused by this virus.

Outreach CPTs
CPTModifier
(if needed)
Quantity
DescriptionComments
87798​​
​1
​Amplified probe technique, each organism​
Synonyms/Keywords


EBV (Epstein-Barr Virus)

EBV Detection by PCR (Polymerase Chain Reaction)

EBV Detection by PCR (Polymerase Chain Reaction), Spinal Fluid

EBV Detection by Real-Time PCR

Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction

Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction), CSF

Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR)

Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR), CSF

Epstein-Barr Virus Detection by Real-Time PCR

Infectious Mononucleosis

LightCycler EBV

PCR (Polymerase Chain Reaction)

PCR, Epstein-Barr​


Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

Submit only 1 of the following specimens: ​ ​​ ​

Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis), amniotic, or ocular.

Collection Instructions: Do not centrifuge.​


​Sterile screwcap 5-mL plastic vial
Sterile container
​0.5 mL
​0.3 mL


Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate


​Sterile screwcap 5-mL plastic vial
Sterile container
​1.5 mL​
​1.0 mL


Swab

Sources: Eye and upper respiratory (nasal, throat).

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)


​Multimicrobe media (M4-RT) and Eswabs



Bone marrow
Additional Information: Clotted specimens will be rejected.
​EDTA Lavender TopTube (LTT)

​0.5 mL

Tissue

Sources: Brain, colon, kidney, liver, lung, cornea, etc.

Collection Instructions: Submit only fresh tissue.​


​Multimicrobe medium (M4-RT)
​Sterile container containing 1-2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)
Entire collection
2 x 2-mm biopsy
Collection Processing

Specimen source is required.

Specimen Stability Information
Specimen TypeTemperature

Time
Varies​

​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Formalin-fixed and paraffin-embedded tissues
Heat-inactivated specimens
Clotted EDTA blood



Interference

A negative result does not eliminate the possibility of Epstein-Barr virus (EBV) infection of the central nervous system.

This assay may detect viremia or viral shedding in asymptomatic individuals. However, this assay is only to be used for patients with a clinical history and symptoms consistent with EBV infection and must be interpreted in the context of the clinical picture.



Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens

Diagnosis of disease due to EBV

This test should not be used to screen asymptomatic patients.



Reference Range Information
Performing Location
Reference Range
Mayo Clinic Laboratories​

Negative

Reference values apply to all ages.​


Interpretation

​Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus. EBV DNA is not detected in CSF from patients without CNS disease caused by this virus.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test Performed
Analytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories
Monday through Friday
​Same day/1 to 4 days​
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
Quantity
DescriptionComments
87798​​
​1
​Amplified probe technique, each organism​
For most current information refer to the Marshfield Laboratory online reference manual.