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26514 Cytomegalovirus (CMV) Molecular Detection, PCR, Varies (CMVPV)

Cytomegalovirus (CMV) Molecular Detection, PCR, Varies (CMVPV)
Test Code: MISC
Synonyms/Keywords

​CMV Detection by Real-Time PCR

Cytomegalovirus, PCR

LightCycler CMV

PCR (Polymerase Chain Reaction)​


Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA

This test is not intended for the monitoring of CMV disease progression.

This test should not be used to screen asymptomatic patients.



Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Submit only 1 of the following specimens:​​​​ ​ ​ ​ ​ ​



Body Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular .

Collection Instructions: Do not centrifuge.

​Sterile, screwcap, 5-mL aliquot tube
Sterile container
​0.5 mL
 0.3 mL


Respiratory fluid

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate​.

Collection Instructions: Do not centrifuge.


Sterile, screwcap, 5-mL aliquot tube
Sterile container
​1.5 mL
​1.0 mL


Genital swab

Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


Multimicrobe media (M4-RT) and ESwabs


Swab

Sources: Dermal, eye, nasal, saliva, or throat.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


​Multimicrobe media (M4-RT) and ESwabs


Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline


​Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)

Entire collection
2 x 2-mm biopsy


​Urine
Collection Instructions: Collect a random urine specimen.
Sterile container
​1.0 mL​
​0.3 mL​
​​
Collection Processing Instructions

​Specimen source is required.

For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.​

Specimen Stability Information
Specimen TypeTemperature
Time
​​
Varies
​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Feces
​Paraffin blocks
​Breast milk
Heat inactivated specimens



Interference

A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.

This assay is only to be used for patients with a clinical history and symptoms consistent with CMV infection and must be interpreted in the context of the clinical picture.



Performing Laboratory Information
Performing LocationDay(s) Test Performed
Analytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 to 4 days
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
Test Information

Infection with cytomegalovirus (CMV) is a significant cause of morbidity and mortality in transplant recipients and other immunocompromised hosts. Specific neurologic syndromes associated with CMV infection include subacute radiculomyelopathy, peripheral neuropathy, and encephalitis.

CMV-associated central nervous system (CNS) disease occurs most commonly in immunocompromised patients. Histologic evidence of CMV infections in autopsy brain tissue was identified in 20% to 40% of AIDS patients. In 2 separate studies, CMV (DNA) was the most common herpesvirus (29/181, 16/49) detected from the cerebrospinal fluid of patients with AIDS. 

CNS infections with CMV can also occur in immunocompetent patients. CMV is a leading cause of congenital viral infections worldwide, and laboratory testing by real-time polymerase chain reaction is useful in the diagnosis of neonatal CMV disease.



Reference Range Information
Performing LocationReference Range
Mayo Clinic Laboratories​​

Negative

Reference values apply to all ages.


Interpretation

Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.

Studies indicate that CMV DNA is not detected by polymerase chain reaction in cerebrospinal fluid from patients without central nervous system disease caused by this virus.



Outreach CPTs
CPTModifier
(if needed)
Quantity
DescriptionComments
87496
​1
​Cytomegalovirus, amplified probe technique
Synonyms/Keywords

​CMV Detection by Real-Time PCR

Cytomegalovirus, PCR

LightCycler CMV

PCR (Polymerase Chain Reaction)​


Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Submit only 1 of the following specimens:​​​​ ​ ​ ​ ​ ​



Body Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular .

Collection Instructions: Do not centrifuge.

​Sterile, screwcap, 5-mL aliquot tube
Sterile container
​0.5 mL
 0.3 mL


Respiratory fluid

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate​.

Collection Instructions: Do not centrifuge.


Sterile, screwcap, 5-mL aliquot tube
Sterile container
​1.5 mL
​1.0 mL


Genital swab

Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


Multimicrobe media (M4-RT) and ESwabs


Swab

Sources: Dermal, eye, nasal, saliva, or throat.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


​Multimicrobe media (M4-RT) and ESwabs


Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline


​Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)

Entire collection
2 x 2-mm biopsy


​Urine
Collection Instructions: Collect a random urine specimen.
Sterile container
​1.0 mL​
​0.3 mL​
​​
Collection Processing

​Specimen source is required.

For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.​

Specimen Stability Information
Specimen TypeTemperature
Time
​​
Varies
​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Feces
​Paraffin blocks
​Breast milk
Heat inactivated specimens



Interference

A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.

This assay is only to be used for patients with a clinical history and symptoms consistent with CMV infection and must be interpreted in the context of the clinical picture.



Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA

This test is not intended for the monitoring of CMV disease progression.

This test should not be used to screen asymptomatic patients.



Reference Range Information
Performing LocationReference Range
Mayo Clinic Laboratories​​

Negative

Reference values apply to all ages.


Interpretation

Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.

Studies indicate that CMV DNA is not detected by polymerase chain reaction in cerebrospinal fluid from patients without central nervous system disease caused by this virus.



For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test Performed
Analytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 to 4 days
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
Quantity
DescriptionComments
87496
​1
​Cytomegalovirus, amplified probe technique
For most current information refer to the Marshfield Laboratory online reference manual.