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26481 Enteric Parasite Panel, NAT

Enteric Parasite Panel, NAT
Test Code: EPPNAT
Synonyms/Keywords

Giardia lamblia, Cryptosporidium, Entamoeba, Stool, Feces, Nucleic Acid Test, Enteric Parasite, ST EPPNAT

Test Components

DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.

Useful For

​Detection of Giardia lamblia, Cryptosporidium hominis, Cryptosporidium parvum and Entamoeba histolytica in patients with gastroenteritis.  Cryptosporidium and Giardia are the most common protozoan parasites encountered in the US and represent 85% of all significant parasites recovered in our laboratory.  Multiple stool PCR tests on the same patient are not necessary to optimize yield, and repeat tests on patients with negative initial results are not indicated.

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No​Feces​Leak-proof container​10% formalin​1 gram​1 gram​1 gram
Collection Processing Instructions

​The preferred specimen is fresh stool in a leak-proof container. Transfer approximately 1 gram of stool specimen to a dry, clean container. Avoid contamination with water or urine.

Acceptable Specimen Types

Feces, Liquid or Soft Raw Stool

Specimen Stability Information
Specimen TypeTemperatureTime
​Feces ​​Ambient​24 hours
​Refrigerated​120 hours
Rejection Criteria
Rectal Swabs
​Feces at room temperature equal to or greater than 24 hours old
​Feces refrigerated equal to or greater than 120 hours old
​Feces submitted in Cary-Blair
​Frozen feces
Duplicate specimen within 24 hours​
Interference

Any inhibition of amplification will be detected through the use of an internal amplification control.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield​Sunday through Friday​6-8 hours*​Nucliec Acid Test

*Testing performed once/day.

  Sample must be received in the lab by 0400 to be tested same day.      

​ ​ ​
Reference Range Information
Performing LocationReference Range
​MarshfieldNegative for Giardia lamblia, Cryptosporidium hominis, Crytposporidium parvum and Entamoeba histolytica
Interpretation

​Negative results in individuals with gastroenteritis may be due to infection by pathogens that are not detected by this test or non-infectious causes. Positive results do not rule out co-infection with other organisms that may also cause gastroenteritis.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87505
Synonyms/Keywords

Giardia lamblia, Cryptosporidium, Entamoeba, Stool, Feces, Nucleic Acid Test, Enteric Parasite, ST EPPNAT

Test Components

DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.

Ordering Applications
Ordering ApplicationDescription
COM​​Enteric Parasite Panel, NAT
​Cerner​Enteric Parasite Panel, NAT
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No​Feces​Leak-proof container​10% formalin​1 gram​1 gram​1 gram
Collection Processing

​The preferred specimen is fresh stool in a leak-proof container. Transfer approximately 1 gram of stool specimen to a dry, clean container. Avoid contamination with water or urine.

Acceptable Specimen Types

Feces, Liquid or Soft Raw Stool

Specimen Stability Information
Specimen TypeTemperatureTime
​Feces ​​Ambient​24 hours
​Refrigerated​120 hours
Rejection Criteria
Rectal Swabs
​Feces at room temperature equal to or greater than 24 hours old
​Feces refrigerated equal to or greater than 120 hours old
​Feces submitted in Cary-Blair
​Frozen feces
Duplicate specimen within 24 hours​
Interference

Any inhibition of amplification will be detected through the use of an internal amplification control.

Useful For

​Detection of Giardia lamblia, Cryptosporidium hominis, Cryptosporidium parvum and Entamoeba histolytica in patients with gastroenteritis.  Cryptosporidium and Giardia are the most common protozoan parasites encountered in the US and represent 85% of all significant parasites recovered in our laboratory.  Multiple stool PCR tests on the same patient are not necessary to optimize yield, and repeat tests on patients with negative initial results are not indicated.

Test Components

DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.

Reference Range Information
Performing LocationReference Range
​MarshfieldNegative for Giardia lamblia, Cryptosporidium hominis, Crytposporidium parvum and Entamoeba histolytica
Interpretation

​Negative results in individuals with gastroenteritis may be due to infection by pathogens that are not detected by this test or non-infectious causes. Positive results do not rule out co-infection with other organisms that may also cause gastroenteritis.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield​Sunday through Friday​6-8 hours*​Nucliec Acid Test

*Testing performed once/day.

  Sample must be received in the lab by 0400 to be tested same day.      

​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87505
For most current information refer to the Marshfield Laboratory online reference manual.