Culture Ref for ID Mycobacterium & Nocardia (CTBID), Culture Referred for Identification, Mycobacterium and Nocardia, Varies (CTBID), Acid-Fast Bacilli (AFB), Aerobic Actinomycetes, Bacillus, Acid-Fast, Culture Referred for Identification, Nocardia (Aerobic Actinomycetes), Culture Referred for Identification, TB (Tuberculosis), Culture, TB (Tuberculosis), Mycobacteria Culture, Mycobacterium tuberculosis (MTB), Nocardia Referred for Identification, Organism Referred for Identification, Mycobacterium, Organism Referred for Identification, Nocardia (Aerobic Actinomycetes), Referral for Identification, Tuberculosis (TB), Tubercle Bacilli: Mycobacterium tuberculosis
Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates
Visible growth of isolate on solid media
Isolate in broth media: > or =3 mL
A minimum volume of 3 mL is recommended in order to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is <3 mL, initial testing may be limited, and increased turnaround time is likely.
Specimen source is required.
Isolate description is required: Gram stain reaction, morphology, tests performed.
Organism must be in pure culture, actively growing. Do not submit mixed cultures.
If the organism is received in mixed culture or contaminated, the report may be delayed or identification may not be possible.
There are approximately 200 recognized species of mycobacteria and more than 100 Nocardia species. Many of these species are human pathogens and, therefore, identification to the species level is important to help guide patient care. In addition, there are other aerobic actinomycete genera that can be human pathogens including, but not limited to, Tsukamurella, Rhodococcus, and Gordonia species.
Nucleic acid hybridization probes are utilized that identify specific ribosomal RNA sequences of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium gordonae. Other Mycobacteria species, Nocardia species and other aerobic actinomycete genera are identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or nucleic acid sequencing of a 500-base pair region of the 16S ribosomal RNA gene.
Organisms growing in pure culture are identified to the species level whenever possible.