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26387 Small Lymphocytic Lymphoma, FISH, Tissue (SLL)

Small Lymphocytic Lymphoma, FISH, Tissue (SLL)
Test Code: SLLSO
Synonyms/Keywords

​CLL, trisomy 12, 11q- (11q deletion) or ATM, 13q- (13q deletion) or LAMP1, 17p- (17p deletion) or TP53, 6q- (6q deletion) or MYB, Nonleukemic form of chronic lymphocytic leukemia (CLL), SLL, Small lymphocytic leukemia (SLL), t(11;14)(q13;q32)-CCND1/IGH, t(14;19)(q32;q13)-IGH/BCL3

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with small lymphocytic lymphoma (SLL) and other low-grade B-cell lymphomas

Distinguishing patients with 11;14 translocations who have mantle cell lymphoma from patients who have SLL

HIGHLIGHTS:This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples from patients with small lymphocytic lymphoma.

TESTING ALGORITHM: 

This test does not include a pathology consult. If a pathology consult is requested, PATHC / Pathology Consultation should be ordered, and the appropriate fluorescence in situ hybridization (FISH) test will be ordered and performed at an additional charge.

Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.

A charge and CPT code is applied for each probe set hybridized, analyzed, and reported.

If the patient is being tracked for known abnormalities, indicate which probes should be used.

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

When an IGH rearrangement is identified, reflex testing with the t(14;19)(q32;q13IGH/BCL3 FISH probe will be performed.

Specimen Requirements
​Submit only one of the following specimens:  ​ ​
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Lymph node​Tissue Block - FFPE​Slides - 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.​See Specimen Required.​See Specimen Required.
​Solid tumor​Tissue block - FFPE​Slides - 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.​See Specimen Required.​See Specimen Required. 
Collection Processing Instructions

​Tissue Block Collection Instructions:  Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slide Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

Specimen Stability Information
Specimen TypeTemperature
​Tissue ​​Ambient (preferred)
​Refrigerated
Rejection Criteria

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. 

Interference

This test is not approved by the U.S. Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.

Fixatives other than formalin (eg, Prefer, Bouin) may not be successful for FISH assays, however nonformalin-fixed samples will not be rejected.

Paraffin-embedded tissues that have been decalcified are generally unsuccessful for FISH analysis. The pathologist reviewing the hematoxylin and eosin-stained slide may find it necessary to cancel testing.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​7 to 10 days​Fluorescence In Situ Hybridization (FISH)
Reference Lab
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​An interpretive report will be provided. 
Interpretation

​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.

A positive result is not diagnostic for small lymphocytic lymphoma, but may provide relevant prognostic information.

The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​88377​1​if 1 probe set
​88377​2​if 2 probe sets
​88377​3​if 3 probe sets
​88377​4​if 4 probe sets
​88377​5 ​if 5 probe sets
​88377​6​if 6 probe sets
​88377​7​if 7 probe sets
​88377​8 ​if 8 probe sets
Synonyms/Keywords

​CLL, trisomy 12, 11q- (11q deletion) or ATM, 13q- (13q deletion) or LAMP1, 17p- (17p deletion) or TP53, 6q- (6q deletion) or MYB, Nonleukemic form of chronic lymphocytic leukemia (CLL), SLL, Small lymphocytic leukemia (SLL), t(11;14)(q13;q32)-CCND1/IGH, t(14;19)(q32;q13)-IGH/BCL3

Ordering Applications
Ordering ApplicationDescription
​COM​Small Lymphocytic Lymphoma FISH, Tissue (SLL)
​Cerner​Small Lymphocytic Lymphoma FISH, Tissue (SLL)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
​Submit only one of the following specimens:  ​ ​
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Lymph node​Tissue Block - FFPE​Slides - 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.​See Specimen Required.​See Specimen Required.
​Solid tumor​Tissue block - FFPE​Slides - 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.​See Specimen Required.​See Specimen Required. 
Collection Processing

​Tissue Block Collection Instructions:  Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slide Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

Specimen Stability Information
Specimen TypeTemperature
​Tissue ​​Ambient (preferred)
​Refrigerated
Rejection Criteria

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. 

Interference

This test is not approved by the U.S. Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.

Fixatives other than formalin (eg, Prefer, Bouin) may not be successful for FISH assays, however nonformalin-fixed samples will not be rejected.

Paraffin-embedded tissues that have been decalcified are generally unsuccessful for FISH analysis. The pathologist reviewing the hematoxylin and eosin-stained slide may find it necessary to cancel testing.

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with small lymphocytic lymphoma (SLL) and other low-grade B-cell lymphomas

Distinguishing patients with 11;14 translocations who have mantle cell lymphoma from patients who have SLL

HIGHLIGHTS:This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples from patients with small lymphocytic lymphoma.

TESTING ALGORITHM: 

This test does not include a pathology consult. If a pathology consult is requested, PATHC / Pathology Consultation should be ordered, and the appropriate fluorescence in situ hybridization (FISH) test will be ordered and performed at an additional charge.

Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.

A charge and CPT code is applied for each probe set hybridized, analyzed, and reported.

If the patient is being tracked for known abnormalities, indicate which probes should be used.

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

When an IGH rearrangement is identified, reflex testing with the t(14;19)(q32;q13IGH/BCL3 FISH probe will be performed.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​An interpretive report will be provided. 
Interpretation

​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.

A positive result is not diagnostic for small lymphocytic lymphoma, but may provide relevant prognostic information.

The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​7 to 10 days​Fluorescence In Situ Hybridization (FISH)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​88377​1​if 1 probe set
​88377​2​if 2 probe sets
​88377​3​if 3 probe sets
​88377​4​if 4 probe sets
​88377​5 ​if 5 probe sets
​88377​6​if 6 probe sets
​88377​7​if 7 probe sets
​88377​8 ​if 8 probe sets
For most current information refer to the Marshfield Laboratory online reference manual.