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26356 COVID 19, Influenza A and B, Respiratory Syncytial Virus (RSV) PCR

COVID 19, Influenza A and B, Respiratory Syncytial Virus (RSV) PCR
Test Code: ABCRNAT
Synonyms/Keywords

​​COVID 19, SARS-CoV-2, Coronavirus, Influenza, RSV

Useful For

​

This is a qualitative molecular test for the detection of nucleic acid of coronavirus 2 (SARS-CoV-2), Influenza virus types A and B and Respiratory Syncytial virus (RSV) in nasopharyngeal swabs from patients presenting with respiratory symptoms.  Combination testing is most useful during periods when these viruses are in known circulation in the community.  Patients may have mild to severe respiratory illness with symptoms of sore throat, cough, fever, headache, congestion or runny nose, shortness of breath and complications including pneumonia.

Refer to the MCHS COVID-19 Test Kit Selection for specimen collection guidance.

All SARS-CoV-2 results are reported to the Wisconsin Electronic Disease Surveillance System (WEDSS).  Confirmed and suspected COVID-19 disease is a Category 1 reportable condition in Wisconsin.

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Nasopharyngeal Swab​UTM Universal Viral Transport, M6 Viral Transport, Xpert VTM​​One nasophryngeal swab​One nasopharyngeal swab​One nasopharyngeal swab
Collection Processing Instructions

Collect a nasopharyngeal specimen using the NP swab. Place swab into the appropriate transport medium and carefully break off the swab shaft against the side of the tube at the score line. Discard the top portion of the swab shaft. Securely tighten the cap of tube.

Transport specimen at 2-8°C to Marshfield Labs as soon as possible.  If specimen cannot be tested within 72 hours of collection, it should be frozen at -70°C or colder until tested. 

Acceptable Body Sites

​Nasopharyngeal

Acceptable Specimen Types

​Nasopharyngeal swab

Specimen Stability Information
Specimen TypeTemperatureTime
​Nasopharyngeal swab ​​Refrigerate​<= 72 hours
​Frozen​> 72 hours
Rejection Criteria
Specimens on cotton or calcium alginate tipped swabs
Swabs with wooden shaft
Specimens in transport media other than those listed above
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield Routine​Monday through Sunday​<= 24 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Marshfield STAT​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Eau Claire​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Park Falls​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Ladysmith​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Rice Lake​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Minocqua​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Neillsville​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Weston​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Beaver Dam
​Monday through Sunday
​3 hours
​Nucleic Acid Test (NAT); Real-Time RT-PCR
​River Region
​Monday through Sunday
​3 hours
​Nucleic Acid Test (NAT); Real-Time RT-PCR
Test Information

Marshfield Labs COVID-19/Influenza Nucleic Acid Test assays are Emergency Use Authorization (EUA) assays performed on multiple platforms.

MCHS Laboratory Summary on SARS-COV-2 Assay Sensitivity and Specificity

The current COVID-19/Influenza/RSV tests used at MCHS have been authorized for use by regulatory authorities including the FDA and CDC.  These tests are highly specific (no false-positives) and are considered to be very sensitive, approaching 100%, during symptomatic disease when high titers of viral target are present, including directly before symptoms appear and following symptom resolution for several days.  Appropriate collection technique is a critical variable for best test performance. 

Assays performed at MCHS use validated gene targets and reagents, strict quality control, and qualified testing personnel.  Results generated are comparable to those produced by public health and academic laboratories nationally. Negative results do not preclude SARS-CoV-2/Influenza/RSV infection and should not be used as the sole basis for patient management decisions.  Negative results must be combined with clinical observations, patient history, and epidemiological information.

Reference Range Information
Performing LocationReference Range
​All Sites​Negative
Interpretation

Positive – Result indicates that the specific virus nucleic acid is detected and suggests infection with the virus.

Negative –Result indicates that the specific virus is not detected.  

Indeterminate - Result is indeterminate due to a PCR inhibitor present in the specimen. Consider repeat specimen if clinically indicated.

Synonyms/Keywords

​​COVID 19, SARS-CoV-2, Coronavirus, Influenza, RSV

Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Nasopharyngeal Swab​UTM Universal Viral Transport, M6 Viral Transport, Xpert VTM​​One nasophryngeal swab​One nasopharyngeal swab​One nasopharyngeal swab
Collection Processing

Collect a nasopharyngeal specimen using the NP swab. Place swab into the appropriate transport medium and carefully break off the swab shaft against the side of the tube at the score line. Discard the top portion of the swab shaft. Securely tighten the cap of tube.

Transport specimen at 2-8°C to Marshfield Labs as soon as possible.  If specimen cannot be tested within 72 hours of collection, it should be frozen at -70°C or colder until tested. 

Acceptable Body Sites

​Nasopharyngeal

Acceptable Specimen Types

​Nasopharyngeal swab

Specimen Stability Information
Specimen TypeTemperatureTime
​Nasopharyngeal swab ​​Refrigerate​<= 72 hours
​Frozen​> 72 hours
Rejection Criteria
Specimens on cotton or calcium alginate tipped swabs
Swabs with wooden shaft
Specimens in transport media other than those listed above
Useful For

​

This is a qualitative molecular test for the detection of nucleic acid of coronavirus 2 (SARS-CoV-2), Influenza virus types A and B and Respiratory Syncytial virus (RSV) in nasopharyngeal swabs from patients presenting with respiratory symptoms.  Combination testing is most useful during periods when these viruses are in known circulation in the community.  Patients may have mild to severe respiratory illness with symptoms of sore throat, cough, fever, headache, congestion or runny nose, shortness of breath and complications including pneumonia.

Refer to the MCHS COVID-19 Test Kit Selection for specimen collection guidance.

All SARS-CoV-2 results are reported to the Wisconsin Electronic Disease Surveillance System (WEDSS).  Confirmed and suspected COVID-19 disease is a Category 1 reportable condition in Wisconsin.

Reference Range Information
Performing LocationReference Range
​All Sites​Negative
Interpretation

Positive – Result indicates that the specific virus nucleic acid is detected and suggests infection with the virus.

Negative –Result indicates that the specific virus is not detected.  

Indeterminate - Result is indeterminate due to a PCR inhibitor present in the specimen. Consider repeat specimen if clinically indicated.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield Routine​Monday through Sunday​<= 24 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Marshfield STAT​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Eau Claire​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Park Falls​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Ladysmith​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Rice Lake​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Minocqua​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Neillsville​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Weston​Monday through Sunday​3 hours​Nucleic Acid Test (NAT); Real-Time RT-PCR
​Beaver Dam
​Monday through Sunday
​3 hours
​Nucleic Acid Test (NAT); Real-Time RT-PCR
​River Region
​Monday through Sunday
​3 hours
​Nucleic Acid Test (NAT); Real-Time RT-PCR
For billing questions, see Contacts
For most current information refer to the Marshfield Laboratory online reference manual.