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26346 Mycobacterial Culture, Blood (CTBBL)

Mycobacterial Culture, Blood (CTBBL)
Test Code: CTBBLSO
Synonyms/Keywords

​Acid-Fast Bacilli (AFB)

AFB (Acid-Fast Bacilli)

Bacillus, Acid-Fast

Culture, TB (Tuberculosis)

MTB (Mycobacterium tuberculosis)

Mycobacteria Culture

Mycobacterium tuberculosis (MTB)

TB (Tuberculosis)

TB (Tuberculosis) Culture

Tubercle Bacilli: Mycobacterium tuberculosis

Tuberculosis (TB)

Useful For

​Diagnosing mycobacteremia

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​​Whole Blood​Green Top Tube (GTT) (sodium or lithium heparin)​SPS/Isolator System​8 to 10 mL per culture​5 mL
Collection Processing Instructions

**All requests must be reviewed before further processing the request.**

Specimen must be processed within 72 hours of collection.

Specimen source is required.​

1. Send specimen in original tube.

2. Isolator System/SPS tubes are acceptable, but not preferred.

3. Draw blood in an Isolator/SPS tube and send 8 mL of whole blood in the original Isolator tube.

4. Note: when sending SPS tube, it must be clearly labeled SPS. If label is obscured, sample may be cancelled, as ACD (yellow top) is not an acceptable tube type.

Specimen Stability Information
Specimen TypeTemperatureTime
​Whole Blood ​​Ambient (preferred)​72 hours
​Refrigerted​72 hours
Rejection Criteria
No specimen should be rejected.
Interference

​Results must be interpreted in conjunction with the patient's history and clinical picture.

A negative result does not rule out mycobacteremia. The organism may be present at quantities below the limit of detection or may be transiently present.

If Mycobacterium genavense is suspected, indicate on request form or contact laboratory. Mycobactin J (an iron supplement) will then be added to the culture to support growth.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​42 days​Continuously Monitored Automated Broth Culture Instrument with Conventional Methods for Identification of Mycobacteria
Reference Lab
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

Negative

If positive, mycobacteria is identified.

A final negative report will be issued after 42 days of incubation.

Interpretation

​A positive result may support the diagnosis of mycobacteremia.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87116​Mycobacterial Culture
​87118​ID MALDI-TOF Mass Spec AFB​​if appropriate
​87150​​ID Mtb Speciation, PCR​if appropriate
​87150​​Mycobacteria Probe Ident, Broth​if appropriate
​87150​​Mycobacteria Probe Ident, Solid ​if appropriate
​87153​Mtb PZA Confirmation, pcnA sequence​if appropriate
​87153​Mycobacteria Identification by Sequencing​if appropriate
​87150​​ID, MTB complex Rapid PCR​if appropriate
Synonyms/Keywords

​Acid-Fast Bacilli (AFB)

AFB (Acid-Fast Bacilli)

Bacillus, Acid-Fast

Culture, TB (Tuberculosis)

MTB (Mycobacterium tuberculosis)

Mycobacteria Culture

Mycobacterium tuberculosis (MTB)

TB (Tuberculosis)

TB (Tuberculosis) Culture

Tubercle Bacilli: Mycobacterium tuberculosis

Tuberculosis (TB)

Ordering Applications
Ordering ApplicationDescription
​COM​Mycobacterial Culture, Blood only (CTBBL)
​Cerner​Mycobacterial Culture, Blood only (CTBBL)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​​Whole Blood​Green Top Tube (GTT) (sodium or lithium heparin)​SPS/Isolator System​8 to 10 mL per culture​5 mL
Collection Processing

**All requests must be reviewed before further processing the request.**

Specimen must be processed within 72 hours of collection.

Specimen source is required.​

1. Send specimen in original tube.

2. Isolator System/SPS tubes are acceptable, but not preferred.

3. Draw blood in an Isolator/SPS tube and send 8 mL of whole blood in the original Isolator tube.

4. Note: when sending SPS tube, it must be clearly labeled SPS. If label is obscured, sample may be cancelled, as ACD (yellow top) is not an acceptable tube type.

Specimen Stability Information
Specimen TypeTemperatureTime
​Whole Blood ​​Ambient (preferred)​72 hours
​Refrigerted​72 hours
Rejection Criteria
No specimen should be rejected.
Interference

​Results must be interpreted in conjunction with the patient's history and clinical picture.

A negative result does not rule out mycobacteremia. The organism may be present at quantities below the limit of detection or may be transiently present.

If Mycobacterium genavense is suspected, indicate on request form or contact laboratory. Mycobactin J (an iron supplement) will then be added to the culture to support growth.

Useful For

​Diagnosing mycobacteremia

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

Negative

If positive, mycobacteria is identified.

A final negative report will be issued after 42 days of incubation.

Interpretation

​A positive result may support the diagnosis of mycobacteremia.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​42 days​Continuously Monitored Automated Broth Culture Instrument with Conventional Methods for Identification of Mycobacteria
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87116​Mycobacterial Culture
​87118​ID MALDI-TOF Mass Spec AFB​​if appropriate
​87150​​ID Mtb Speciation, PCR​if appropriate
​87150​​Mycobacteria Probe Ident, Broth​if appropriate
​87150​​Mycobacteria Probe Ident, Solid ​if appropriate
​87153​Mtb PZA Confirmation, pcnA sequence​if appropriate
​87153​Mycobacteria Identification by Sequencing​if appropriate
​87150​​ID, MTB complex Rapid PCR​if appropriate
For most current information refer to the Marshfield Laboratory online reference manual.