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26256 Mycoplasma hominis, Molecular Detection, PCR, Plasma (MHPRP)

Mycoplasma hominis, Molecular Detection, PCR, Plasma (MHPRP)
Test Code: MHPRPSO
Synonyms/Keywords

​Mycoplasma hominis PCR

Useful For

​Rapid, sensitive, and specific identification of Mycoplasma hominis from plasma

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Plasma EDTAEDTA ​Lavender Top Tube (LTT)​EDTA Royal Blue Top (RBTT), EDTA Pink Top Tube (PTT)​1 mL​0.5 mL
Collection Processing Instructions

​Centrifuge and separate plasma within 24 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime
​Plasma EDTA ​​Refrigerated (preferred)​7 days
​Frozen​7 days
Rejection Criteria
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Interference

​Interfering substances may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings

Since Mycoplasma hominis may be part of the normal flora, results should be interpreted accordingly.

This test does not detect other mycoplasmas or ureaplasmas (including Mycoplasma pneumoniae, a common cause of community acquired pneumonia)

This test is not intended for medicolegal use.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​3 to 4 days​Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.

A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87798​1
Synonyms/Keywords

​Mycoplasma hominis PCR

Ordering Applications
Ordering ApplicationDescription
​COM​Mycoplasma hominis, PCR, P
​Cerner​Mycoplasma hominis, P (MHPRP)
​Centricity​Mycoplasma hominis, P (MHPRP)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Plasma EDTAEDTA ​Lavender Top Tube (LTT)​EDTA Royal Blue Top (RBTT), EDTA Pink Top Tube (PTT)​1 mL​0.5 mL
Collection Processing

​Centrifuge and separate plasma within 24 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime
​Plasma EDTA ​​Refrigerated (preferred)​7 days
​Frozen​7 days
Rejection Criteria
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Interference

​Interfering substances may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings

Since Mycoplasma hominis may be part of the normal flora, results should be interpreted accordingly.

This test does not detect other mycoplasmas or ureaplasmas (including Mycoplasma pneumoniae, a common cause of community acquired pneumonia)

This test is not intended for medicolegal use.

Useful For

​Rapid, sensitive, and specific identification of Mycoplasma hominis from plasma

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.

A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​3 to 4 days​Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87798​1
For most current information refer to the Marshfield Laboratory online reference manual.