Skip Ribbon Commands
Skip to main content
Sign In

26191 Human Papillomavirus (HPV) High-Risk E6/E7, RNA in Situ Hybridization (HPVE6)

Human Papillomavirus (HPV) High-Risk E6/E7, RNA in Situ Hybridization (HPVE6)
Test Code: HPVE6SO
Synonyms/Keywords

​HPV (Human Papillomavirus), HPV E6/E7, GPHPVE6ISH, ISHPVE6E7IISH, ISNEGHPVE6E7ISH, ISRNAHPVE6E7ISH

Useful For

​Stratification of oropharyngeal squamous cell carcinoma. 

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No​Formalin-fixed, paraffin-embedded tissue block​Entire Block
​No​Slides​5 unstained glass, positively charged slides with 5 (+ or -1)-microns formalin-fixed, paraffin-embedded tissue
Collection Processing Instructions

​If additional interpretation or analysis is needed, request PATHC/Pathology Consultation along with this test.

Shipping Instructions:  Attach the green pathology address label included in the kit to the outside of the transport container.

A pathology/diagnostic report and a brief history are required.

Mayo Labs suggests supply:  Pathology Packaging Kit (T554)

Specimen Stability Information
Specimen TypeTemperature
​Special ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Tissue/Other

Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Interference

​Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​5 days​In situ hybridization on sections of paraffin-embedded tissue. 
Reference Lab
Test Information

​This assay is intended to identify the presence of human papillomavirus (HPV) E6/E7 transcripts from high-risk genotypes. This test has been shown to be more sensitive than HPV DNA in situ hybridization (ISH). Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC. An indication for this test is p16 expression by immunohistochemistry and negative HPV DNA ISH. In this instance, consideration should be given to the possibility of a false-negative HPV DNA ISH result.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic LaboratoriesResults are reported as positive or negative for types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82.​
Interpretation

​This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​88365​1
​88364​(If additional ISH)
Synonyms/Keywords

​HPV (Human Papillomavirus), HPV E6/E7, GPHPVE6ISH, ISHPVE6E7IISH, ISNEGHPVE6E7ISH, ISRNAHPVE6E7ISH

Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No​Formalin-fixed, paraffin-embedded tissue block​Entire Block
​No​Slides​5 unstained glass, positively charged slides with 5 (+ or -1)-microns formalin-fixed, paraffin-embedded tissue
Collection Processing

​If additional interpretation or analysis is needed, request PATHC/Pathology Consultation along with this test.

Shipping Instructions:  Attach the green pathology address label included in the kit to the outside of the transport container.

A pathology/diagnostic report and a brief history are required.

Mayo Labs suggests supply:  Pathology Packaging Kit (T554)

Specimen Stability Information
Specimen TypeTemperature
​Special ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Tissue/Other

Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Interference

​Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Useful For

​Stratification of oropharyngeal squamous cell carcinoma. 

Reference Range Information
Performing LocationReference Range
​Mayo Clinic LaboratoriesResults are reported as positive or negative for types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82.​
Interpretation

​This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​5 days​In situ hybridization on sections of paraffin-embedded tissue. 
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​88365​1
​88364​(If additional ISH)
For most current information refer to the Marshfield Laboratory online reference manual.