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26169 Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies (MPRP)

Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies (MPRP)
Test Code: MPRPSO
Synonyms/Keywords

​​​Mycoplasma pneumoniae, Mycoplasmoides pneumoniae

Useful For

​Diagnosis of infections due to Mycoplasmoides pneumoniae

Specimen Requirements
Submit only 1 of the following specimens:​ ​ ​ ​
Specimen TypePreferred Container/Tube​
Acceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Respiratory: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
​Sterile Container
​1 mL
​Swab: Throat, nasal or nasopharyngeal
​Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)​Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, UTM or ESwab​1 Swab​1 Swab
​Fluid: Pleural, pericardial, or cerebrospinal
​Sterile Container
​0.5 mL
Collection Processing Instructions

​Specimen source is required.

Swab Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder. 

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies ​​
​Refrigerated (preferred)​7 days
​Frozen​7 days
Rejection Criteria
​Cotton or calcium alginate-tipped swab​
​Wooden shaft swab
​Transport swab containing gel or charcoal
​Port-a-Cul tube
​Anaerobic fluid vials
​Dry swab (no pledget or sponge)



Interference

​This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalvelor lavage fluid) and pleural/chest fluid, pericardial fluid and cerebrospinal fluid. 

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​3 to 4 days​Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Test Information

Mycoplasmoides pneumoniae, previously Mycoplasma pneumoniae, is a small bacterium transmitted via organism-containing droplets. It is a cause of upper respiratory infection, pharyngitis, and tracheobronchitis, particularly in children, and has been associated with approximately 20% of cases of community acquired pneumonia. Central nervous system and cardiac manifestations are probably the most frequent extrapulmonary complications of infections due to M pneumoniae. The disease is usually self-limited although severe disease has been reported in immunocompromised patients.

Identification of M pneumoniae by culture-based methods is time consuming and insensitive. Serology based assays for M pneumoniae have several drawbacks. The development of IgM antibodies takes approximately 1 week and the IgM response in adults may be variable or it may be decreased in immunosuppressed individuals. Confirmation of the disease may be dependent on the observation of a 4-fold rise in IgG antibody titers between acute and convalescent specimens, several weeks following the initial onset of illness, providing clinical utility only for retrospective testing. Real-time polymerase chain reaction testing offers a rapid and sensitive option for detection of M pneumoniae DNA from clinical specimens.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

​ A positive result indicates the presence of Mycoplasmoides pneumoniae

A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below of detection of the assay. 

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87581
Synonyms/Keywords

​​​Mycoplasma pneumoniae, Mycoplasmoides pneumoniae

Ordering Applications
Ordering ApplicationDescription

​Cerner
​Mycoplasma pneu by PCR (MPRP)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Submit only 1 of the following specimens:​ ​ ​ ​
Specimen TypePreferred Container/Tube​
Acceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Respiratory: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
​Sterile Container
​1 mL
​Swab: Throat, nasal or nasopharyngeal
​Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)​Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, UTM or ESwab​1 Swab​1 Swab
​Fluid: Pleural, pericardial, or cerebrospinal
​Sterile Container
​0.5 mL
Collection Processing

​Specimen source is required.

Swab Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder. 

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies ​​
​Refrigerated (preferred)​7 days
​Frozen​7 days
Rejection Criteria
​Cotton or calcium alginate-tipped swab​
​Wooden shaft swab
​Transport swab containing gel or charcoal
​Port-a-Cul tube
​Anaerobic fluid vials
​Dry swab (no pledget or sponge)



Interference

​This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalvelor lavage fluid) and pleural/chest fluid, pericardial fluid and cerebrospinal fluid. 

Useful For

​Diagnosis of infections due to Mycoplasmoides pneumoniae

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Not applicable
Interpretation

​ A positive result indicates the presence of Mycoplasmoides pneumoniae

A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below of detection of the assay. 

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​3 to 4 days​Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87581
For most current information refer to the Marshfield Laboratory online reference manual.