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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Bacteria PCR & Sequence (BRBPS)
Test Code: BRBPSSO
Synonyms/Keywords
Ribosomal RNA, Broad range, 16S, Bacterial Sequencing , PCR
Useful For
This test is used for detection and identification of bacteria (including mycobacteria) in normally sterile specimens after routine culture fails to exhibit growth.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Tissue, fresh (bone, lymph node, heart valve, brain, viscera, organ, lung)   ​Sterile container ​5 mm (3) ​5 mm (3) ​5 mm (3)
​Tissue Block ​Paraffin-embedded 2 - 10 micro sections ​2 - 10 micro sections
Fluid (CSF, vitreous, pleural, abdominal, peritoneal, ascites, pericardial, pelvic) ​Sterile container ​1 mL ​0.5 mL ​0.5 mL
​Synovial Fluid ​EDTA Lavender Top Tube (LTT) ​1 mL ​0.5 mL ​0.5 mL
Collection Processing Instructions
Submit specimens collected using sterile technique. Fresh tissue is preferred over formalin-fixed, paraffin-embedded tissue.
Specimen Stability Information
Specimen Type Temperature Time
​Tissue ​Frozen or Refrigerate ​< 14 days
​Tissue Block ​Ambient or Refrigerate ​N/A
​Fluid ​Frozen or Refrigerate ​< 14 days
​Synovial Fluid ​Frozen or Refrigerate ​< 14 days
Rejection Criteria
Tissue in formalin, formaldehyde, acetone, or any other fluid
Fluids collected in anticoagulants other than EDTA: green top (heparin) tube, blue top (citrate) tube, yellow top (ACD) tube, serum separator tube (SST)
Blood, bone marrow, decalcified bone, slides
​Specimens submitted on swabs
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Tuesday, Thursday ​7 days 16S Ribosomal RNA Gene PCR followed by Sanger Sequencing of the Amplified Product
Reference Lab
Test Information
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​No Bacterial DNA Detected
Interpretation
A positive broad-range PCR/sequencing result indicates that bacterial nucleic acid of the specified organisms was detected, which may be due to bacterial infection or environmental or contaminating nucleic acids in the specimen. A negative broad-range PCR/sequencing result indicates the absence of detectable bacterial (including mycobacterial) nucleic acids in the specimen, but does not rule-out false-negative results that may occur due to sampling error, sequence variability underlying the primers, the presence of bacterial nucleic acids in quantities less than the limit of detection of the assay, or inhibition of PCR. If PCR testing appears to be negative but there is evidence of PCR inhibition, testing will be repeated. If inhibition is again detected, the result will be reported as "PCR inhibition present." This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87801 ​1 ​Broad Range Bacterial PCR and Sequencing
87153​ ​1 ​Bacterial Identification by Sequencing ​If needed
​87798 ​1 ​Identification by PCR ​If needed
Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Synonyms/Keywords
Ribosomal RNA, Broad range, 16S, Bacterial Sequencing , PCR
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Tissue, fresh (bone, lymph node, heart valve, brain, viscera, organ, lung)   ​Sterile container ​5 mm (3) ​5 mm (3) ​5 mm (3)
​Tissue Block ​Paraffin-embedded 2 - 10 micro sections ​2 - 10 micro sections
Fluid (CSF, vitreous, pleural, abdominal, peritoneal, ascites, pericardial, pelvic) ​Sterile container ​1 mL ​0.5 mL ​0.5 mL
​Synovial Fluid ​EDTA Lavender Top Tube (LTT) ​1 mL ​0.5 mL ​0.5 mL
Collection Processing Instructions
Submit specimens collected using sterile technique. Fresh tissue is preferred over formalin-fixed, paraffin-embedded tissue.
Specimen Stability Information
Specimen Type Temperature Time
​Tissue ​Frozen or Refrigerate ​< 14 days
​Tissue Block ​Ambient or Refrigerate ​N/A
​Fluid ​Frozen or Refrigerate ​< 14 days
​Synovial Fluid ​Frozen or Refrigerate ​< 14 days
Rejection Criteria
Tissue in formalin, formaldehyde, acetone, or any other fluid
Fluids collected in anticoagulants other than EDTA: green top (heparin) tube, blue top (citrate) tube, yellow top (ACD) tube, serum separator tube (SST)
Blood, bone marrow, decalcified bone, slides
​Specimens submitted on swabs
Useful For
This test is used for detection and identification of bacteria (including mycobacteria) in normally sterile specimens after routine culture fails to exhibit growth.
Test Information
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​No Bacterial DNA Detected
Interpretation
A positive broad-range PCR/sequencing result indicates that bacterial nucleic acid of the specified organisms was detected, which may be due to bacterial infection or environmental or contaminating nucleic acids in the specimen. A negative broad-range PCR/sequencing result indicates the absence of detectable bacterial (including mycobacterial) nucleic acids in the specimen, but does not rule-out false-negative results that may occur due to sampling error, sequence variability underlying the primers, the presence of bacterial nucleic acids in quantities less than the limit of detection of the assay, or inhibition of PCR. If PCR testing appears to be negative but there is evidence of PCR inhibition, testing will be repeated. If inhibition is again detected, the result will be reported as "PCR inhibition present." This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Tuesday, Thursday ​7 days 16S Ribosomal RNA Gene PCR followed by Sanger Sequencing of the Amplified Product
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87801 ​1 ​Broad Range Bacterial PCR and Sequencing
87153​ ​1 ​Bacterial Identification by Sequencing ​If needed
​87798 ​1 ​Identification by PCR ​If needed
Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
For most current information refer to the Marshfield Laboratory online reference manual.