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26122 Bacterial Culture, Cystic Fibrosis with Antimicrobial Susceptibilities (CFRCS)

Bacterial Culture, Cystic Fibrosis with Antimicrobial Susceptibilities (CFRCS)
Test Code: MISC
Useful For
Detection of aerobic bacterial pathogens in specimens from patients with cystic fibrosis
           
Determining the in vitro antimicrobial susceptibility of potentially pathogenic aerobic bacteria, if appropriate
 

NOTES:
-Specimens should be submitted ONLY at Marshfield Center, SATURDAY-THURSDAY between the hours of 00:01-24:00.  Specimens submitted at other times or at other centers may be rejected.
Contact the Microbiology lab to discuss exceptions to this policy.

Not to be ordered on Outreach clients.

Specimen Requirements
Submit only 1 of the following specimens:
 
 
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Sputum, expectorated or induced​Sterile, screw-capped container​2 mL​2 mL
Bronchial aspirate or washing, bronchoalveolar lavage, endotracheal or tracheal​Sterile, screw-capped container​​​2 mL​2 mL
Throat​​ESwab​Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium​One swab
Collection Processing Instructions
Deliver specimens to the laboratory as soon as possible. When culture is delayed, successful isolation of bacterial pathogens is less likely, due to the overgrowth of usual oropharyngeal flora.
 

Shipping Instructions: Specimen must arrive at Mayo within 48 hours of collection.

Specimens collected on Friday will not arrive at Mayo within 48 hours and will be rejected.

Specimen Stability Information
Specimen Type Temperature Time
​All Specimens ​Refrigerated ​48 hours
Rejection Criteria
Specimen >48 hours old
​Pooled 24 hour samples
​Dry swab
 
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Sunday ​5 to 12 days ​Conventional Culture Technique
Reference Lab
Test Information
Life expectancy of patients with CF has increased steadily over the past 50 years, in large part due to improvements in the management of lung disease in this patient population. Still, chronic lung infection is responsible for 75% to 85% of deaths in patients with CF. Appropriate treatment for the causative organism can reduce morbidity and mortality.
 
The number of microbial species associated with CF lung disease is relatively limited. These include Pseudomonas aeruginosa (mucoid and nonmucoid), Staphylococcus aureus, Burkholderia cepacia complex, Stenotrophomonas maltophilia, other nonfermenting gram-negative rods, Haemophilus influenzae, and Streptococcus pneumoniae. Nontuberculous mycobacteria, common respiratory viruses and Aspergillus species may also play a role in CF lung disease. This culture, which is specifically designed for CF patients, utilizes conventional and additional selective media (compared to non-CF respiratory cultures) to isolate bacteria commonly associated with pulmonary disease in CF patients.
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories

​No growth or usual flora

Identification of probable pathogens

Interpretation
A negative test result is no growth of bacteria or growth of only usual flora. A negative result does not rule out all causes of infectious lung disease.
 
Organisms associated with lower respiratory tract infections are reported.
 
For positive test results, pathogenic bacteria are identified. Patients with cystic fibrosis (CF) may be colonized or chronically infected by some organisms over a long period of time, therefore, positive results must be interpreted in conjunction with previous findings and the clinical picture to appropriately evaluate results.
 
Ordering Applications
Ordering Application Description
​Centricity Culture, Cystic Fibrosis w/susceptibility (CFRC)
​Cerner ​Culture, Cystic Fibrosis w/susceptibility (CFRC)
​COM ​Culture, Cystic Fibrosis w/susceptibility (CFRC)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Submit only 1 of the following specimens:
 
 
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Sputum, expectorated or induced​Sterile, screw-capped container​2 mL​2 mL
Bronchial aspirate or washing, bronchoalveolar lavage, endotracheal or tracheal​Sterile, screw-capped container​​​2 mL​2 mL
Throat​​ESwab​Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium​One swab
Collection Processing
Deliver specimens to the laboratory as soon as possible. When culture is delayed, successful isolation of bacterial pathogens is less likely, due to the overgrowth of usual oropharyngeal flora.
 

Shipping Instructions: Specimen must arrive at Mayo within 48 hours of collection.

Specimens collected on Friday will not arrive at Mayo within 48 hours and will be rejected.

Specimen Stability Information
Specimen Type Temperature Time
​All Specimens ​Refrigerated ​48 hours
Rejection Criteria
Specimen >48 hours old
​Pooled 24 hour samples
​Dry swab
 
Useful For
Detection of aerobic bacterial pathogens in specimens from patients with cystic fibrosis
           
Determining the in vitro antimicrobial susceptibility of potentially pathogenic aerobic bacteria, if appropriate
 

NOTES:
-Specimens should be submitted ONLY at Marshfield Center, SATURDAY-THURSDAY between the hours of 00:01-24:00.  Specimens submitted at other times or at other centers may be rejected.
Contact the Microbiology lab to discuss exceptions to this policy.

Not to be ordered on Outreach clients.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories

​No growth or usual flora

Identification of probable pathogens

Interpretation
A negative test result is no growth of bacteria or growth of only usual flora. A negative result does not rule out all causes of infectious lung disease.
 
Organisms associated with lower respiratory tract infections are reported.
 
For positive test results, pathogenic bacteria are identified. Patients with cystic fibrosis (CF) may be colonized or chronically infected by some organisms over a long period of time, therefore, positive results must be interpreted in conjunction with previous findings and the clinical picture to appropriately evaluate results.
 
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Sunday ​5 to 12 days ​Conventional Culture Technique
Reference Lab
For billing questions, see Contacts
For most current information refer to the Marshfield Laboratory online reference manual.