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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Mycoplasma hominis, PCR (MHRP)
Test Code: MHRPSO
Useful For
Detection of Mycoplasma hominis from synovial fluid, genitourinary, reproductive, lower respiratory sources, pleural/chest fluid, pericardial fluid and wound specimens.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media or Swab in Transport Media ​One swab ​One swab
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​1-10 mL ​1 mL
​Urine ​Sterile, screw-capped container ​10 mL ​2 mL
​Sputum, Tracheal Secretions, Bronchial wash or lavage, Lung Fluid; or Nasal washings (only infants <3 months) ​Sterile, screw-capped container ​​M4, M5 or M6 Transport Media ​1-2 mL ​1 mL
​Synovial Fluid ​​Sterile, screw-capped container ​EDTA tube ​1 mL
​Tissue ​​Sterile, screw-capped container ​5 mm
Collection Processing Instructions
​Specimens should be delivered to the laboratory as soon as possible.
 
Do not use calcium alginate or wooden shaft swabs.
 
Samples should be taken without contact with antiseptics or lubricants.
 
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​All Specimens ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​3 days Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
Test Information
Mycoplasma hominis has been associated with a number of clinically significant infections, although it is also part of the normal genital flora.
 
M hominis may be found in the respiratory specimens and spinal fluid of neonates. Although the clinical significance of such findings is often unclear, as spontaneous clinical recovery may occur without specific treatment, in premature infants, clinical manifestations of meningoencephalitis have been reported.
 
M hominis may play a role in some cases of pelvic inflammatory disease, usually in combination with other organisms. M hominis may be isolated from amniotic fluid of women with preterm labor, premature rupture of membranes, spontaneous term labor, or chorioamnionitis; there is evidence that it may be involved in postpartum fever or fever following abortion, usually as a complication of endometritis.
 
M hominis has rarely been associated with septic arthritis (including prosthetic joint infection), pyelonephritis, intraabdominal infection, wound infection, endocarditis, central nervous system infection (including meningoencephalitis, brain abscess, central nervous system shunt infection and subdural empyema), pneumonia, and infected pleural and pericardial effusions.
 
PCR detection of M hominis is sensitive, specific, and provides same-day results. Although this organism can occasionally be detected in routine plate cultures, this is neither a rapid nor a sensitive approach to detection. Specialized cultures are more time consuming than the described PCR assay. The described PCR assay has replaced conventional culture for M hominis at Mayo Medical Laboratories due to its speed and equivalent performance to culture.
 
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​Negative
Interpretation
A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.
 
A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as falsely negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87798 ​1
Ordering Applications
Ordering Application Description
​Centricity ​Mycoplasma hominis, PCR (MHRP)
​Cerner ​Mycoplasma hominis, PCR (MHRP)
​COM ​Mycoplasma hominis, PCR (MHRP)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media or Swab in Transport Media ​One swab ​One swab
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​1-10 mL ​1 mL
​Urine ​Sterile, screw-capped container ​10 mL ​2 mL
​Sputum, Tracheal Secretions, Bronchial wash or lavage, Lung Fluid; or Nasal washings (only infants <3 months) ​Sterile, screw-capped container ​​M4, M5 or M6 Transport Media ​1-2 mL ​1 mL
​Synovial Fluid ​​Sterile, screw-capped container ​EDTA tube ​1 mL
​Tissue ​​Sterile, screw-capped container ​5 mm
Collection Processing Instructions
​Specimens should be delivered to the laboratory as soon as possible.
 
Do not use calcium alginate or wooden shaft swabs.
 
Samples should be taken without contact with antiseptics or lubricants.
 
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
​All Specimens ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
Useful For
Detection of Mycoplasma hominis from synovial fluid, genitourinary, reproductive, lower respiratory sources, pleural/chest fluid, pericardial fluid and wound specimens.
Test Information
Mycoplasma hominis has been associated with a number of clinically significant infections, although it is also part of the normal genital flora.
 
M hominis may be found in the respiratory specimens and spinal fluid of neonates. Although the clinical significance of such findings is often unclear, as spontaneous clinical recovery may occur without specific treatment, in premature infants, clinical manifestations of meningoencephalitis have been reported.
 
M hominis may play a role in some cases of pelvic inflammatory disease, usually in combination with other organisms. M hominis may be isolated from amniotic fluid of women with preterm labor, premature rupture of membranes, spontaneous term labor, or chorioamnionitis; there is evidence that it may be involved in postpartum fever or fever following abortion, usually as a complication of endometritis.
 
M hominis has rarely been associated with septic arthritis (including prosthetic joint infection), pyelonephritis, intraabdominal infection, wound infection, endocarditis, central nervous system infection (including meningoencephalitis, brain abscess, central nervous system shunt infection and subdural empyema), pneumonia, and infected pleural and pericardial effusions.
 
PCR detection of M hominis is sensitive, specific, and provides same-day results. Although this organism can occasionally be detected in routine plate cultures, this is neither a rapid nor a sensitive approach to detection. Specialized cultures are more time consuming than the described PCR assay. The described PCR assay has replaced conventional culture for M hominis at Mayo Medical Laboratories due to its speed and equivalent performance to culture.
 
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​Negative
Interpretation
A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.
 
A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as falsely negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​3 days Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87798 ​1
For most current information refer to the Marshfield Laboratory online reference manual.