Parental Prenatal Array Prep Test (PPAP)

Test Code: PPAPSO

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Useful For

Preparing parental blood samples for possible confirmation testing if an abnormality is detected on the prenatal array sample.

DNA extraction of the maternal blood sample used for maternal cell contamination testing.

This test can only be ordered on the parents when a prenatal microarray test has been ordered on the fetal sample.

This test code contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.

When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.

Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.

Specimen Requirements

*This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA
Fasting Required	Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
No	Whole Blood	Green Top Tube Sodium Heparin (GTT) AND EDTA Lavendar Top Tube (LTT)		GTT-4 mL LTT-3 mL	GTT- 2 mL LTT-2 mL

*This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA

Fasting Required

Specimen Type

Preferred Container/Tube

Acceptable Container/Tube

Specimen Volume

Specimen Minimum Volume
(allows for 1 repeat)

Pediatric Minimum Volume
(no repeat)

Whole Blood

Green Top Tube Sodium Heparin (GTT) AND
EDTA Lavendar Top Tube (LTT)

GTT-4 mL

LTT-3 mL

GTT- 2 mL

LTT-2 mL

Collection Processing Instructions

This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA.

1. Invert several times to mix blood.
2. Send specimens in original tubes. Do Not aliquot.

Necessary Information:

1. The reason for testing is required.

2. Include the name listed on the prenatal sample that was submitted for CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue.

Specimen Stability Information

Specimen Type	Temperature
Whole Blood	Ambient (preferred)
Whole Blood	Refrigerate

Rejection Criteria

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Report Available	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Friday	Varies	DNA Extraction/ Cell Culture

Reference Lab

Mayo Clinic Laboratories

Test Information

In order to interpret equivocal array results on a prenatal sample (amniotic fluid or chorionic villus), parental studies are performed to determine if the abnormality detected on the prenatal array is inherited or de novo.

Maternal cell contamination testing is performed on the maternal blood and prenatal specimen to detect the presence of maternal cells in the fetal sample.

Reference Range Information

An interpretaive report will be provided

Interpretation

No interpretation will be provided. This test is for specimen processing only at Mayo Labs.

Ordering Applications

Ordering Application	Description
Cerner	Parental Sample Prep for Prenatal Microarray Testing (PPAP)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.