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25625 T-Cell Receptor Gene Rearrange, PCR, Bld (TCGR)

T-Cell Receptor Gene Rearrange, PCR, Bld (TCGR)
Test Code: TCGRSO
Synonyms/Keywords
​Lymphoma vs Benign Process, Reactive Lymphocytic Process, T-Cell Gene Rearrangement, T Cell Gene Rearrangement
Useful For
​Determining whether a T-cell population is polyclonal or monoclonal
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​4 mL ​1 mL
Collection Processing Instructions

​Specimen must arrive within 7 days (168 hours) of draw.

Include relevant clinical information and cytogenetics results, if available.

Include Hematopathology Patient Information Sheet.
(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​ Ambient (preferred) ​7 days
​Refrigerate ​7 days
Rejection Criteria
Gross Hemolysis
​Moderately to Severely Clotted
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​5 days ​Polymerase Chain Reaction (PCR)
Reference Lab
Test Information

The T-cell receptor (TCR) genes (alpha, beta, delta, and gamma) are comprised of numerous, discontinuous coding segments that somatically rearrange to produce heterodimeric cell surface T-cell receptors, either alpha/beta (90%-95% of T cells) or gamma/delta (5%-10% of T cells). With rare exceptions (eg, some neoplastic B-lymphoid proliferations), other cell types retain the germline configuration of the TCR genes without rearrangement.

The marked diversity of somatic TCR-gene rearrangements is important for normal immune functions but also serves as a valuable marker to distinguish abnormal T-cell proliferations from reactive processes. A monoclonal expansion of a T-cell population will result in the predominance of a single TCR-gene rearrangement pattern. In contrast, reactive T-cell expansions are polyclonal (or multiclonal), with no single clonotypic population predominating in the population of T cells. These distributive differences in both TCR sequence and genomic rearrangement fragment sizes can be detected by molecular techniques (ie, polymerase chain reaction) and used to determine if a population of T cells shows monoclonal or polyclonal features.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​​Positive, negative, or indeterminate for a clonal T-cell population
Interpretation
​An interpretive report will be provided. Results will be characterized as positive, negative, or indeterminate for a clonal T-cell population. In the appropriate clinicopathologic setting, a monoclonal result is associated with a neoplastic proliferation of T cells.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81340 ​TCB gene rearrangement, PCR
​81342 ​TCG at gene rearrangement analysis
Classification
Synonyms/Keywords
​Lymphoma vs Benign Process, Reactive Lymphocytic Process, T-Cell Gene Rearrangement, T Cell Gene Rearrangement
Ordering Applications
Ordering Application Description
​COM ​T-Cell Receptor Rearrange PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​4 mL ​1 mL
Collection Processing

​Specimen must arrive within 7 days (168 hours) of draw.

Include relevant clinical information and cytogenetics results, if available.

Include Hematopathology Patient Information Sheet.
(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​ Ambient (preferred) ​7 days
​Refrigerate ​7 days
Rejection Criteria
Gross Hemolysis
​Moderately to Severely Clotted
Useful For
​Determining whether a T-cell population is polyclonal or monoclonal
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​​Positive, negative, or indeterminate for a clonal T-cell population
Interpretation
​An interpretive report will be provided. Results will be characterized as positive, negative, or indeterminate for a clonal T-cell population. In the appropriate clinicopathologic setting, a monoclonal result is associated with a neoplastic proliferation of T cells.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​5 days ​Polymerase Chain Reaction (PCR)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81340 ​TCB gene rearrangement, PCR
​81342 ​TCG at gene rearrangement analysis
Classification
For most current information refer to the Marshfield Laboratory online reference manual.