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25624 T-Cell Receptor Gene Rearr, PCR, Varies (TCGRV)

T-Cell Receptor Gene Rearr, PCR, Varies (TCGRV)
Test Code: TCGRVSO
Synonyms/Keywords
​Lymphoma vs Benign Process, Reactive Lymphocytic Process, T-Cell Gene Rearrangement, TCGRV, T Cell Gene Rearrangement, T Cell Clonality
Useful For
Determining whether a T-cell population is polyclonal or monoclonal
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Body Fluid ​Sterile Container ​5 mL ​1 mL
​Paraffin-embedded bone marrow aspirate clot ​Paraffin block
​Frozen Tissue ​Plastic Container ​100 mg ​50 mg
​Paraffin-embedded tissue ​Paraffin block
​Spinal fluid ​Sterile Vial ​10 mL ​1 mL
​Extracted DNA from blood or bone marrow ​1.5 to 2 mL tube with indication of volume and concentration of DNA ​Entire Specimen ​50 microliter at
20 ng/mcL
Collection Processing Instructions
Body fluid or spinal fluid specimens must arrive within 4 days (96 hours) of collection.

If sending body fluid:
1. If the volume is large, pellet cells prior to sending.
2. Send less volume at ambient temperature or as a frozen cell pellet.

Include Hematopathology Patient Information Sheet.
(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Specimen Stability Information
Specimen Type Temperature Time
​Body Fluid ​ ​ ​Ambient ​96 hours
​Refrigerate ​96 hours
​Frozen ​96 hours
​Cell Pellet ​Frozen ​96 hours
​Paraffin-embedded bone marrow aspirate clot ​ ​Ambient ​Indefinitely
​Refrigerate ​​Indefinitely
​Frozen Tissue ​Frozen ​​Indefinitely
​Parrafin-embedded tissue ​ ​ Ambient ​​Indefinitely
​Refrigerate ​​Indefinitely
​Frozen ​​Indefinitely
​Spinal Fluid ​ Ambient ​96 hours
​Refrigerate ​96 hours
​Extracted DNA from blood or bone marrow ​ Ambient ​​Indefinitely
​Refrigerate ​​Indefinitely
Rejection Criteria
Bone marrow biopsies or paraffin shavings
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​7 days Polymerase Chain Reaction (PCR)
Test Information

The T-cell receptor (TCR) genes (alpha, beta, delta, and gamma) are comprised of numerous, discontinuous coding segments that somatically rearrange to produce heterodimeric cell surface TCR, either alpha/beta (90%-95% of T cells) or gamma/delta (5%-10% of T cells). With rare exceptions (eg, some neoplastic B-lymphoid proliferations), other cell types retain the germline configuration of the TCR genes without rearrangement.

The marked diversity of somatic TCR-gene rearrangements is important for normal immune functions but also serves as a valuable marker to distinguish abnormal T-cell proliferations from reactive processes. A monoclonal expansion of a T-cell population will result in the predominance of a single TCR-gene rearrangement pattern. In contrast, reactive T-cell expansions are polyclonal (or multiclonal), with no single clonotypic population predominating in the population of T cells. These distributive differences in both TCR sequence and genomic rearrangement fragment sizes can be detected by molecular techniques (ie, polymerase chain reaction) and used to determine if a population of T cells shows monoclonal or polyclonal features.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Positive, negative, or indeterminate for a clonal T-cell population
Interpretation
An interpretive report will be provided. Results will be characterized as positive, negative, or indeterminate for a clonal T-cell population. In the appropriate clinicopathologic setting, a monoclonal result is associated with a neoplastic proliferation of T cells.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81340 ​TCB gene rearrangement, PCR
​81342 ​​TCG at gene rearrangement analysis
Classification
Synonyms/Keywords
​Lymphoma vs Benign Process, Reactive Lymphocytic Process, T-Cell Gene Rearrangement, TCGRV, T Cell Gene Rearrangement, T Cell Clonality
Ordering Applications
Ordering Application Description
​COM ​T-Cell Receptor Rearr, Varies
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Body Fluid ​Sterile Container ​5 mL ​1 mL
​Paraffin-embedded bone marrow aspirate clot ​Paraffin block
​Frozen Tissue ​Plastic Container ​100 mg ​50 mg
​Paraffin-embedded tissue ​Paraffin block
​Spinal fluid ​Sterile Vial ​10 mL ​1 mL
​Extracted DNA from blood or bone marrow ​1.5 to 2 mL tube with indication of volume and concentration of DNA ​Entire Specimen ​50 microliter at
20 ng/mcL
Collection Processing
Body fluid or spinal fluid specimens must arrive within 4 days (96 hours) of collection.

If sending body fluid:
1. If the volume is large, pellet cells prior to sending.
2. Send less volume at ambient temperature or as a frozen cell pellet.

Include Hematopathology Patient Information Sheet.
(http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Specimen Stability Information
Specimen Type Temperature Time
​Body Fluid ​ ​ ​Ambient ​96 hours
​Refrigerate ​96 hours
​Frozen ​96 hours
​Cell Pellet ​Frozen ​96 hours
​Paraffin-embedded bone marrow aspirate clot ​ ​Ambient ​Indefinitely
​Refrigerate ​​Indefinitely
​Frozen Tissue ​Frozen ​​Indefinitely
​Parrafin-embedded tissue ​ ​ Ambient ​​Indefinitely
​Refrigerate ​​Indefinitely
​Frozen ​​Indefinitely
​Spinal Fluid ​ Ambient ​96 hours
​Refrigerate ​96 hours
​Extracted DNA from blood or bone marrow ​ Ambient ​​Indefinitely
​Refrigerate ​​Indefinitely
Rejection Criteria
Bone marrow biopsies or paraffin shavings
Useful For
Determining whether a T-cell population is polyclonal or monoclonal
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Positive, negative, or indeterminate for a clonal T-cell population
Interpretation
An interpretive report will be provided. Results will be characterized as positive, negative, or indeterminate for a clonal T-cell population. In the appropriate clinicopathologic setting, a monoclonal result is associated with a neoplastic proliferation of T cells.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​7 days Polymerase Chain Reaction (PCR)
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81340 ​TCB gene rearrangement, PCR
​81342 ​​TCG at gene rearrangement analysis
Classification
For most current information refer to the Marshfield Laboratory online reference manual.