The undiluted, 1:10, and 1:100 diluted Prolactin results are compared.
ABSENT: If after correction for dilution factor all results are similar, no hook effect is suspected. Undiluted Prolactin result is reported.
PRESENT: If the 1:10 and/or 1:100 dilutions are not similar to the undiluted sample, hook effect is likely. Sample will continue to be diluted until similar results from one dilution to the next are observed. Diluted Prolactin result is reported along with the final dilution performed.