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25592 Hook Effect Prolactin

Hook Effect Prolactin
Test Code: HEPRL
Synonyms/Keywords
​Hook Effect, Prolactin
Test Components
​Sample will be analyzed undiluted (1:1), as well as at 1:10, 1:100 and 1:1000 dilutions. Three results will be reported as follows.
HEPRL:   Hook Effect Prolactin (Presence or Absence of Hook Effect)
PROLAC: Prolactin (Final Prolactin result reported)
PRLDIL:  Hook Effect Final Dilution
Useful For
​Determine if hook effect is contributing to a Prolactin reported result.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Serum ​Serum Separator Tube (SST) ​Red Top Tube (RTT), Lithium-heparin Plasma Separator Tube (PST) ​0.5 mL ​0.4 mL ​0.3 mL
Collection Processing Instructions
​Samples collected in a RTT must be removed from the clot wihin 1 hour for storage or transport. Samples collected in SST or PST must be removed from the primary tube wihin 4 hours for transport.
Specimen Stability Information
Specimen Type Temperature Time
​Serum/Plasma ​ ​Refrigerated ​48 hours
​Frozen ​30 days
Interference
​Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Sunday through Saturday ​4 hours ​Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur
Reference Range Information
Performing Location Reference Range
​Marshfield:  Hook Effect Prolactin
​ABSENT (no hook effect)
​Prolactin
​Female (non-pregnant):      2.8 - 29.2 ng/mL
            (pregnant):             9.7 - 208.5 ng/mL
            (post-menopausal): 1.8 - 20.3 ng/mL
 
Male:     2.1-17.7 ng/mL
Interpretation

​The undiluted, 1:10, and 1:100 diluted Prolactin results are compared.

ABSENT: If after correction for dilution factor all results are similar, no hook effect is suspected.  Undiluted Prolactin result is reported.

PRESENT: If the 1:10 and/or 1:100 dilutions are not similar to the undiluted sample, hook effect is likely.  Sample will continue to be diluted until similar results from one dilution to the next are observed.  Diluted Prolactin result is reported along with the final dilution performed.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​84146 ​ ​1 ​Prolactin ​
Synonyms/Keywords
​Hook Effect, Prolactin
Test Components
​Sample will be analyzed undiluted (1:1), as well as at 1:10, 1:100 and 1:1000 dilutions. Three results will be reported as follows.
HEPRL:   Hook Effect Prolactin (Presence or Absence of Hook Effect)
PROLAC: Prolactin (Final Prolactin result reported)
PRLDIL:  Hook Effect Final Dilution
Ordering Applications
Ordering Application Description
​Cerner ​Hook Effect Prolactin
​Centricity ​Hook Effect Prolactin
​COM ​Hook Effect Prolactin
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Serum ​Serum Separator Tube (SST) ​Red Top Tube (RTT), Lithium-heparin Plasma Separator Tube (PST) ​0.5 mL ​0.4 mL ​0.3 mL
Collection Processing
​Samples collected in a RTT must be removed from the clot wihin 1 hour for storage or transport. Samples collected in SST or PST must be removed from the primary tube wihin 4 hours for transport.
Specimen Stability Information
Specimen Type Temperature Time
​Serum/Plasma ​ ​Refrigerated ​48 hours
​Frozen ​30 days
Interference
​Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies.
Useful For
​Determine if hook effect is contributing to a Prolactin reported result.
Test Components
​Sample will be analyzed undiluted (1:1), as well as at 1:10, 1:100 and 1:1000 dilutions. Three results will be reported as follows.
HEPRL:   Hook Effect Prolactin (Presence or Absence of Hook Effect)
PROLAC: Prolactin (Final Prolactin result reported)
PRLDIL:  Hook Effect Final Dilution
Reference Range Information
Performing Location Reference Range
​Marshfield:  Hook Effect Prolactin
​ABSENT (no hook effect)
​Prolactin
​Female (non-pregnant):      2.8 - 29.2 ng/mL
            (pregnant):             9.7 - 208.5 ng/mL
            (post-menopausal): 1.8 - 20.3 ng/mL
 
Male:     2.1-17.7 ng/mL
Interpretation

​The undiluted, 1:10, and 1:100 diluted Prolactin results are compared.

ABSENT: If after correction for dilution factor all results are similar, no hook effect is suspected.  Undiluted Prolactin result is reported.

PRESENT: If the 1:10 and/or 1:100 dilutions are not similar to the undiluted sample, hook effect is likely.  Sample will continue to be diluted until similar results from one dilution to the next are observed.  Diluted Prolactin result is reported along with the final dilution performed.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Sunday through Saturday ​4 hours ​Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​84146 ​ ​1 ​Prolactin ​
For most current information refer to the Marshfield Laboratory online reference manual.