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25512 Methadone and Metabolite, S (MDNS)

Methadone and Metabolite, S (MDNS)
Test Code: MDNSSO
Synonyms/Keywords
​Methadone (Dolophine)
Test Components
​Methadone and Metabolite
Useful For

Compliance monitoring of methadone

Assessment of methadone toxicity

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum Red ​Red Top Tube (RTT) ​0.5 mL ​0.2 mL
Collection Processing Instructions
​Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​Refrigerated (preferred) ​14 days
​Ambient ​14 days
​Frozen ​14 days
Rejection Criteria
Specimens collected in serum separator tube.
Interference

Methadone has a wide therapeutic index and dose-dependent toxicity. As a result, routine drug monitoring is not indicated in all patients.

Specimens collected in serum gel tubes are not acceptable because the drug can absorb on the gel and lead to falsely decreased concentrations.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Wednesday, Friday ​2 to 5 days Liquid Chromatography-Tandem Mass Spectrometry (​LC-MS/MS)
Reference Lab
Reference Range Information
​Not established ​
Interpretation

​There is a significant overlap between the reported therapeutic and toxic concentrations of methadone in blood specimens. 

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
80358​ ​1 ​Methadone
Synonyms/Keywords
​Methadone (Dolophine)
Test Components
​Methadone and Metabolite
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum Red ​Red Top Tube (RTT) ​0.5 mL ​0.2 mL
Collection Processing
​Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​Refrigerated (preferred) ​14 days
​Ambient ​14 days
​Frozen ​14 days
Rejection Criteria
Specimens collected in serum separator tube.
Interference

Methadone has a wide therapeutic index and dose-dependent toxicity. As a result, routine drug monitoring is not indicated in all patients.

Specimens collected in serum gel tubes are not acceptable because the drug can absorb on the gel and lead to falsely decreased concentrations.

Useful For

Compliance monitoring of methadone

Assessment of methadone toxicity

Test Components
​Methadone and Metabolite
Reference Range Information
​Not established ​
Interpretation

​There is a significant overlap between the reported therapeutic and toxic concentrations of methadone in blood specimens. 

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Wednesday, Friday ​2 to 5 days Liquid Chromatography-Tandem Mass Spectrometry (​LC-MS/MS)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
80358​ ​1 ​Methadone
For most current information refer to the Marshfield Laboratory online reference manual.