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25385 Amniotic Fluid Culture/Genetic Test (CULAF)

Amniotic Fluid Culture/Genetic Test (CULAF)
Test Code: AFCGTSO
Synonyms/Keywords
Amniotic Fluid Culture for Genetic Testing, Cell culture Amniotic fluid, Culture for Genetic Testing Amniotic Fluid, Genetic Testing Culture Amniotic Fluid, Saving cells for possible genetic testing
Useful For
Producing amniocyte cultures that can be used for genetic analysis
 
Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Amniotic Fluid ​Sterile Amniotic Fluid Container ​10.0 mL ​5.0 mL
Collection Processing Instructions
Provide a reason for referral and gestational age with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
 
Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.
 
1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with other cytogenetic testing such as CHRAF / Chromosome Analysis, Amniotic Fluid or CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, a total of 25 to 30 mL will be needed.
2. Place the tubes in a Styrofoam container.
3. Fill remaining space with packing material.
4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
5. Bloody specimens are undesirable.
 
The additional tests desired must be indicated on the request form that accompanies the specimen. 
Specimen Stability Information
Specimen Type Temperature
​Amniotic Fluid ​ ​Ambient Send as soon as possible, no defined time per Mayo​
​Refrigerated (preferred)
Send as soon as possible, no defined time per Mayo​
Rejection Criteria

​All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Interference

Interfering factors:

-Inadequate amount of specimen may not permit adequate analysis

-Exposure of the specimen to temperature extremes (freezing or > 30 degrees C) may destroy cells and interfere with attempts to culture cells

-Improper packaging may result in broken, leaky, and contaminated specimens during transport

-Transport time should not exceed 2 days

-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday 23 to 24 days
The specimen is split into 8 to 10 separate culture dishes. The cells are grown on coverslips in culture media for 4 to 10 days. The cultures are trypsinized into 2 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks. (In The AGT Cytogenetics Laboratory Manual Fourth edition. Edited by MS Arsham, MJ Barch, HJ Lawce. John Wiley and Sons Inc 2017, 175-178)
Reference Lab
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88235 Tissue culture for amniotic fluid​
​88240 ​Cryopreservation
Synonyms/Keywords
Amniotic Fluid Culture for Genetic Testing, Cell culture Amniotic fluid, Culture for Genetic Testing Amniotic Fluid, Genetic Testing Culture Amniotic Fluid, Saving cells for possible genetic testing
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Amniotic Fluid ​Sterile Amniotic Fluid Container ​10.0 mL ​5.0 mL
Collection Processing
Provide a reason for referral and gestational age with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
 
Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.
 
1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with other cytogenetic testing such as CHRAF / Chromosome Analysis, Amniotic Fluid or CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, a total of 25 to 30 mL will be needed.
2. Place the tubes in a Styrofoam container.
3. Fill remaining space with packing material.
4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
5. Bloody specimens are undesirable.
 
The additional tests desired must be indicated on the request form that accompanies the specimen. 
Specimen Stability Information
Specimen Type Temperature
​Amniotic Fluid ​ ​Ambient Send as soon as possible, no defined time per Mayo​
​Refrigerated (preferred)
Send as soon as possible, no defined time per Mayo​
Rejection Criteria

​All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Interference

Interfering factors:

-Inadequate amount of specimen may not permit adequate analysis

-Exposure of the specimen to temperature extremes (freezing or > 30 degrees C) may destroy cells and interfere with attempts to culture cells

-Improper packaging may result in broken, leaky, and contaminated specimens during transport

-Transport time should not exceed 2 days

-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems

Useful For
Producing amniocyte cultures that can be used for genetic analysis
 
Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday 23 to 24 days
The specimen is split into 8 to 10 separate culture dishes. The cells are grown on coverslips in culture media for 4 to 10 days. The cultures are trypsinized into 2 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks. (In The AGT Cytogenetics Laboratory Manual Fourth edition. Edited by MS Arsham, MJ Barch, HJ Lawce. John Wiley and Sons Inc 2017, 175-178)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88235 Tissue culture for amniotic fluid​
​88240 ​Cryopreservation
For most current information refer to the Marshfield Laboratory online reference manual.