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25299 CMV DNA Detection and Quantification by RT-PCR, Plasma (CMVQN)

CMV DNA Detection and Quantification by RT-PCR, Plasma (CMVQN)
Test Code: CMVQUSO
Synonyms/Keywords
​Cytomegalovirus detection by PCR
CMV by PCR
CMV DNA quant
CMV viral load
Useful For

 Detection and quantification of CMV viremia

 Monitoring CMV disease progression and response to antiviral therapy

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma ​Lavender Top Tube (LTT) ​ ​1.5 mL ​0.6 mL ​
Collection Processing Instructions

Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes)​.

Aliquot plasma into plastic vial.  ​

Shipping Instructions:

Ship specimen frozen on dry ice only.

If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice.

Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​Frozen (preferred)

​84 days

​Refrigerated​6 days
Rejection Criteria
Samples not collected in EDTA
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Saturday ​1 to 3 days ​Real-Time Polymerase Chain Reaction
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Undetected
Interpretation

The quantification range of this assay is 35 to 10,000,000 IU/mL (1.54 log to 7.00 log IU/mL), with a 95% or higher limit of detection at 35 IU/mL.

A result of "Undetected" indicates the absence of cytomegalovirus (CMV) DNA in the plasma (see Cautions below).

A result of "<35 IU/mL (<1.54 log IU/mL)" indicates that CMV DNA is detected in the plasma, but the assay cannot accurately quantify the CMV DNA present below this level.

A quantitative value (reported in IU/mL and log IU/mL) indicates the level of CMV DNA (ie, viral load) present in the plasma.

A result of ">10,000,000 IU/mL (>7.00 log IU/mL)" indicates that CMV DNA level present in plasma is above 10,000,000 IU/mL (7.00 log IU/mL), and the assay cannot accurately quantify CMV DNA present above this level.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87497 ​ ​1 ​ ​
Synonyms/Keywords
​Cytomegalovirus detection by PCR
CMV by PCR
CMV DNA quant
CMV viral load
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma ​Lavender Top Tube (LTT) ​ ​1.5 mL ​0.6 mL ​
Collection Processing

Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes)​.

Aliquot plasma into plastic vial.  ​

Shipping Instructions:

Ship specimen frozen on dry ice only.

If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice.

Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​Frozen (preferred)

​84 days

​Refrigerated​6 days
Rejection Criteria
Samples not collected in EDTA
Useful For

 Detection and quantification of CMV viremia

 Monitoring CMV disease progression and response to antiviral therapy

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Undetected
Interpretation

The quantification range of this assay is 35 to 10,000,000 IU/mL (1.54 log to 7.00 log IU/mL), with a 95% or higher limit of detection at 35 IU/mL.

A result of "Undetected" indicates the absence of cytomegalovirus (CMV) DNA in the plasma (see Cautions below).

A result of "<35 IU/mL (<1.54 log IU/mL)" indicates that CMV DNA is detected in the plasma, but the assay cannot accurately quantify the CMV DNA present below this level.

A quantitative value (reported in IU/mL and log IU/mL) indicates the level of CMV DNA (ie, viral load) present in the plasma.

A result of ">10,000,000 IU/mL (>7.00 log IU/mL)" indicates that CMV DNA level present in plasma is above 10,000,000 IU/mL (7.00 log IU/mL), and the assay cannot accurately quantify CMV DNA present above this level.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Saturday ​1 to 3 days ​Real-Time Polymerase Chain Reaction
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87497 ​ ​1 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.