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25298 Pregnenolone, Serum (PREGN)

Pregnenolone, Serum (PREGN)
Test Code: PREGNSO
Useful For

​- An ancillary test for congenital adrenal hyperplasia, particularly in situations in which a diagnosis of 21-hydrolase and 11-hydrolase deficiency have been ruled out. 

- Confirming a diagnosis of 3-beta-hydroxy dehydrogenase deficiency.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separatory Tube (SST) ​1.0 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ Frozen ​28 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Tuesday, Thursday ​2 days Liquid Chromatography-Tandem Mass Spectrometry (​LC-MS/MS)
Reference Lab
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratory

CHILDREN*

Males

0-6 years: not established

7-9 years: <206 ng/dL

10-12 years: <152 ng/dL

13-15 years: 18-197 ng/dL

16-17 years: 17-228 ng/dL

Tanner Stages

Stage I: <157 ng/dL

Stage II: <144 ng/dL

Stage III: <215 ng/dL

Stage IV-V: 19-201 ng/dL

 

Females

0-6 years: not established

7-9 years: <151 ng/dL

10-12 years: 19-220 ng/dL

13-15 years: 22-210 ng/dL

16-17 years: 22-229 ng/dL

Tanner Stages

Stage I: <172 ng/dL

Stage II: 22-229 ng/dL

Stage III: 34-215 ng/dL

Stage IV-V: 26-235 ng/dL

 

ADULTS

> or =18 years: 33-248 ng/dL

*Kushnir MM, Rockwood AL, Roberts WL, et al: Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids. Clin Chem 2006;52(8):1559-1567

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84140 ​1
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separatory Tube (SST) ​1.0 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ Frozen ​28 days
Useful For

​- An ancillary test for congenital adrenal hyperplasia, particularly in situations in which a diagnosis of 21-hydrolase and 11-hydrolase deficiency have been ruled out. 

- Confirming a diagnosis of 3-beta-hydroxy dehydrogenase deficiency.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratory

CHILDREN*

Males

0-6 years: not established

7-9 years: <206 ng/dL

10-12 years: <152 ng/dL

13-15 years: 18-197 ng/dL

16-17 years: 17-228 ng/dL

Tanner Stages

Stage I: <157 ng/dL

Stage II: <144 ng/dL

Stage III: <215 ng/dL

Stage IV-V: 19-201 ng/dL

 

Females

0-6 years: not established

7-9 years: <151 ng/dL

10-12 years: 19-220 ng/dL

13-15 years: 22-210 ng/dL

16-17 years: 22-229 ng/dL

Tanner Stages

Stage I: <172 ng/dL

Stage II: 22-229 ng/dL

Stage III: 34-215 ng/dL

Stage IV-V: 26-235 ng/dL

 

ADULTS

> or =18 years: 33-248 ng/dL

*Kushnir MM, Rockwood AL, Roberts WL, et al: Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids. Clin Chem 2006;52(8):1559-1567

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Tuesday, Thursday ​2 days Liquid Chromatography-Tandem Mass Spectrometry (​LC-MS/MS)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84140 ​1
For most current information refer to the Marshfield Laboratory online reference manual.