These testing algorithms are of utility in evaluating patients for lynch syndrome, when suspected by either clinical or histopathologic parameters; see the Lynch Syndrome Testing Algorithms below.
Lynch Syndrome Algorithm for Colorectal Carcinoma
Lynch Syndrome Algorithm for Endometrial Carcinoma
1) Complete a histology requisition. Form must be compete and all slides/blocks labeled or testing will be delayed.
2) If applicable, include Surgical Pathology report containing pertinent patient history, tissue source, and diagnosis.
Reason for Referral: Possible diagnosis of Lynch syndrome. Evaluate specimen for evidence of defective DNA mismatch repair protein expression.IHC using monoclonal antibodies and a polymer detection system to determine the expression or absence thereof, for the following: hMLH-1, hMSH-2, hMSH-6, and PMS2. Lymphocytes and non-neoplastic epithelium exhibit strong nuclear staining to serve as a positive internal control.
Lynch Syndrome Testing Algorithm for Colorectal Carcinoma
Lynch Syndrome Testing Algorithm for Endometrial Carcinoma
Interpretive Surgical Pathology Report
This test is only available with interpretation.The presence or absence of each marker serves as prognostic and predictive markers for the management of colonic or endometrial cancer.Normal tissue is requested, as lymphocytes and non-neoplastic epithelium exhibit strong nuclear staining, which serves as a positive internal control for each protein.Note: This DNA Mismatch Repair Protein panel is not a genetic test, but rather a screening tool for stratifying the risk of having an inherited cancer predisposition syndrome and identifies patients who may benefit from subsequent genetic testing.