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25202 Allergen, Trout, IgE (TROT)

Allergen, Trout, IgE (TROT)
Test Code: ALTROSO
Synonyms/Keywords
​Chub Salmon, Pacific Salmon, Rainbow Trout, Coho Salmon, King Salmon, Pink Salmon, Oncorhynchus mykiss, Salmo Gairdnier
Useful For

Establishing a diagnosis of an allergy to trout

Defining the allergen responsible for eliciting signs and symptoms

Identifying allergens:

-Responsible for allergic disease and/or anaphylactic episode

-To confirm sensitization prior to beginning immunotherapy

-To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Serum Separator Tube (SST) Red Top Tube (RTT) ​0.5 mL ​0.3 mL
Collection Processing Instructions

​For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information
Specimen Type Temperature​Time
​Serum ​ ​Refrigerate (preferred)​14 days
​Frozen​90 days
Interference

Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.

Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum, and results must be interpreted in the clinical context.

False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases.​

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday 1 day ​Fluorescence Enzyme Immunoassay (FEIA)
Reference Lab
Reference Range Information

Performing LocationReference Range












​Mayo Clinic Laboratories
ClassIgE kU/LInterpretation
0<0.10Negative
0/1 0.10-0.34Borderline/equivocal
10.35-0.69Equivocal
20.70-3.49Positive
33.50-17.4Positive
417.5-49.9Strongly positive
550.0-99.9Strongly positive
6> or =100Strongly positive

Reference values apply to all ages.


Interpretation

​Detection of IgE antibodies in serum (Class 1 or greater) indicates an increased likelihood of allergic disease as opposed to other etiologies and defines the allergens that may be responsible for eliciting signs and symptoms.

The level of IgE antibodies in serum varies directly with the concentration of IgE antibodies expressed as a class score or kU/L.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86003
Synonyms/Keywords
​Chub Salmon, Pacific Salmon, Rainbow Trout, Coho Salmon, King Salmon, Pink Salmon, Oncorhynchus mykiss, Salmo Gairdnier
Ordering Applications
Ordering Application Description
​Centricity ​Allergen, Trout, IgE
​Cerner ​None
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Serum Separator Tube (SST) Red Top Tube (RTT) ​0.5 mL ​0.3 mL
Collection Processing

​For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information
Specimen Type Temperature​Time
​Serum ​ ​Refrigerate (preferred)​14 days
​Frozen​90 days
Interference

Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.

Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum, and results must be interpreted in the clinical context.

False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases.​

Useful For

Establishing a diagnosis of an allergy to trout

Defining the allergen responsible for eliciting signs and symptoms

Identifying allergens:

-Responsible for allergic disease and/or anaphylactic episode

-To confirm sensitization prior to beginning immunotherapy

-To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens 

Reference Range Information

Performing LocationReference Range












​Mayo Clinic Laboratories
ClassIgE kU/LInterpretation
0<0.10Negative
0/1 0.10-0.34Borderline/equivocal
10.35-0.69Equivocal
20.70-3.49Positive
33.50-17.4Positive
417.5-49.9Strongly positive
550.0-99.9Strongly positive
6> or =100Strongly positive

Reference values apply to all ages.


Interpretation

​Detection of IgE antibodies in serum (Class 1 or greater) indicates an increased likelihood of allergic disease as opposed to other etiologies and defines the allergens that may be responsible for eliciting signs and symptoms.

The level of IgE antibodies in serum varies directly with the concentration of IgE antibodies expressed as a class score or kU/L.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday 1 day ​Fluorescence Enzyme Immunoassay (FEIA)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86003
For most current information refer to the Marshfield Laboratory online reference manual.