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25016 Blastomyces Quantitative Antigen, Serum (316)

Blastomyces Quantitative Antigen, Serum (316)
Test Code: BLSAGSO
Synonyms/Keywords
​​Blastomyces Dermatitidis QNT AG
Useful For

Aid in the diagnosis of blastomycosis

Monitor the response to therapy and to determine when treatment can be modified or stopped.

Monitor Blastomyces antigen levels to help determine relapse of disease.

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Minimum Volume
(allows for 1 repeat)
​Serum

​Red Top Tube (RTT)  

Serum Separator Tube (SST) ​1.2 mL
​Plasma​EDTA Lavender Top Tube (LTT)​

​Sodium-heparin OR Lithium-heparin Green Top Tube (GTT)

OR

Sodium citrate tube (BTT)

​1.2 mL
Collection Processing Instructions

Serum:
Collect serum specimens in serum separator or red top tube.
Allow blood to clot for 30 minutes, then centrifuge.
Pipette serum into a plastic screw cap vial.

Plasma:
Collect plasma specimens in an EDTA, heparin or sodium citrate tube.
Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.

​​

​Ship to arrive Monday through Saturday using a next day delivery service.  

Samples may be shipped on dry ice, frozen ice packs, or ambient.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma ​ ​ Room temperature​  14 days​
​Refrigerate (preferred)  14 days​
​Frozen 2 months
Rejection Criteria

Received cold or room temperature >14 days old 

Non-pipettable specimens, tissue, biopsy, sputum, bronchial brush, tracheal aspirate, FNA, bone marrow, aspirate or stool

Samples in transport media, fixative or Isolator tubes
Interference

It is possible that specimens positive for Candida tropicalis, Coccidioides immitis, Coccidioides posadasii, Histoplasma capsulatum, Histoplasma duboisii, Paracoccidioides brasiliensis, Talaromyces marneffei and Aspergillus nidulans may cross-react with the Blastomyces Antigen EIA and test positive.


A variety of interfering substances, as follows, were investigated for each specimen type and none affected the Blastomyces Antigen EIA sensitivity or specificity.

  • Serum: protein, bilirubin, cholesterol, triglycerides, hemoglobin
  • Plasma: protein, bilirubin, cholesterol, triglycerides, hemoglobin, lithium heparin, sodium citrate
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Miravista Diagnostics Monday through Saturday​ 1 day​

Quantitative sandwich enzyme immunoassay (EIA)​

Serum is pre-treated to improve accuracy

Reference Lab
Miravista Diagnostics
Test Information

The MVista® Blastomyces Antigen Quantitative EIA can detect and quantify the amount of Blastomyces antigen in patient samples.

During validation testing, the sensitivity was found to be 95.00% and specificity 100% with an assay cutoff of 0.31 ng/mL.

​Negative results do not exclude blastomycosis. Testing of both urine and serum offers the highest sensitivity. Failure of the antigen to rise does not exclude relapse.

​​Positive samples may require confirmation which could extend TAT.

Reference Range Information
Reference Interval:  None Detected
Reportable Range:  0.31 ng/mL – 20.00 ng/mL​
Results above 20.00 ng/mL are reported as 'Positive, Above the Limit of Quantification' 

When tested in cultures of 10⁵ – 10⁶ organisms/mL, cross-reactions occurred with Histoplasma spp., Coccidioides spp., Paracoccidioides brasiliensis, Talaromyces marneffei, Aspergillus nidulans, and Candida tropicalis.
Interpretation

None Detected: <0.31 ng/mL
Positive: ≥0.31 ng/mL – 20.00 ng/mL
Positive Above the Limit of Quantifications
Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

Results should be correlated with clinical presentation and history

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87449 ​ ​1
 ​ ​
Synonyms/Keywords
​​Blastomyces Dermatitidis QNT AG
Ordering Applications
Ordering Application Description
​Cerner
 ​Blastomyces Quantitative Antigen, Serum (316)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Minimum Volume
(allows for 1 repeat)
​Serum

​Red Top Tube (RTT)  

Serum Separator Tube (SST) ​1.2 mL
​Plasma​EDTA Lavender Top Tube (LTT)​

​Sodium-heparin OR Lithium-heparin Green Top Tube (GTT)

OR

Sodium citrate tube (BTT)

​1.2 mL
Collection Processing

Serum:
Collect serum specimens in serum separator or red top tube.
Allow blood to clot for 30 minutes, then centrifuge.
Pipette serum into a plastic screw cap vial.

Plasma:
Collect plasma specimens in an EDTA, heparin or sodium citrate tube.
Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.

​​

​Ship to arrive Monday through Saturday using a next day delivery service.  

Samples may be shipped on dry ice, frozen ice packs, or ambient.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma ​ ​ Room temperature​  14 days​
​Refrigerate (preferred)  14 days​
​Frozen 2 months
Rejection Criteria

Received cold or room temperature >14 days old 

Non-pipettable specimens, tissue, biopsy, sputum, bronchial brush, tracheal aspirate, FNA, bone marrow, aspirate or stool

Samples in transport media, fixative or Isolator tubes
Interference

It is possible that specimens positive for Candida tropicalis, Coccidioides immitis, Coccidioides posadasii, Histoplasma capsulatum, Histoplasma duboisii, Paracoccidioides brasiliensis, Talaromyces marneffei and Aspergillus nidulans may cross-react with the Blastomyces Antigen EIA and test positive.


A variety of interfering substances, as follows, were investigated for each specimen type and none affected the Blastomyces Antigen EIA sensitivity or specificity.

  • Serum: protein, bilirubin, cholesterol, triglycerides, hemoglobin
  • Plasma: protein, bilirubin, cholesterol, triglycerides, hemoglobin, lithium heparin, sodium citrate
Useful For

Aid in the diagnosis of blastomycosis

Monitor the response to therapy and to determine when treatment can be modified or stopped.

Monitor Blastomyces antigen levels to help determine relapse of disease.

Reference Range Information
Reference Interval:  None Detected
Reportable Range:  0.31 ng/mL – 20.00 ng/mL​
Results above 20.00 ng/mL are reported as 'Positive, Above the Limit of Quantification' 

When tested in cultures of 10⁵ – 10⁶ organisms/mL, cross-reactions occurred with Histoplasma spp., Coccidioides spp., Paracoccidioides brasiliensis, Talaromyces marneffei, Aspergillus nidulans, and Candida tropicalis.
Interpretation

None Detected: <0.31 ng/mL
Positive: ≥0.31 ng/mL – 20.00 ng/mL
Positive Above the Limit of Quantifications
Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

Results should be correlated with clinical presentation and history

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Miravista Diagnostics Monday through Saturday​ 1 day​

Quantitative sandwich enzyme immunoassay (EIA)​

Serum is pre-treated to improve accuracy

Reference Lab
Miravista Diagnostics
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87449 ​ ​1
 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.