Red Top Tube (RTT)
Sodium-heparin OR Lithium-heparin Green Top Tube (GTT)
Sodium citrate tube (BTT)
Collect serum or plasma in a preferred or acceptable tube listed above. Allow blood serum samples to clot for 30 minutes, then centrifuge. Pipette serum into a plastic leak-proof container. Centrifuge blood plasma samples for 15 minutes and pipette plasma into a plastic leak-proof container.
Ship to arrive Monday through Friday using a next day delivery service. May be shipped ambient.
Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
Quantitative sandwich enzyme immunoassay (EIA)
Serum is pre-treated to improve accuracy
Cross-reactions are seen with histoplasmosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidiooidomycosis, rarely in aspergillosis and possibly sporotrichosis. Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.
Negative results do not exclude blastomycosis. Testing of both urine and serum offers the highest sensitivity. Failure of the antigen to rise does not exclude relapse.
The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.
*New positives may require confirmation
Positive Below the Limit of Quantification: Results above the cutoff for positivity, but below 0.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable. Results will be confirmed and reported the next business day.
Positive: 0.2-14.7 ng/mL
Positive Above the Limit of Quantification: Results greater than 14.7 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.