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25016 Blastomyces Quantitative Antigen, Serum (316)

Blastomyces Quantitative Antigen, Serum (316)
Test Code: BLSAGSO
Synonyms/Keywords
​Blastomyces Dermatitidis QNT AG
Useful For
​Aid in diagnosis of blastomycosis
Aid in monitoring to determine when treatment can be discontinued
Aid in diagnosis of relapse
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Minimum Volume
(allows for 1 repeat)
​Serum

​Red Top Tube (RTT)  

Serum Separator Tube (SST) ​1.2 mL
​Plasma​EDTA Lavender Top Tube (LTT)

​Sodium-heparin OR Lithium-heparin Green Top Tube (GTT)

OR

Sodium citrate tube (BTT)

​1.2 mL
Collection Processing Instructions

​Collect serum or plasma in a preferred or acceptable tube listed above.   Allow blood serum samples to clot for 30 minutes, then centrifuge. Pipette serum into a plastic leak-proof container.  Centrifuge blood plasma samples for 15 minutes and pipette plasma into a plastic leak-proof container.

​Ship to arrive Monday through Friday using a next day delivery service.  May be shipped ambient.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma ​ ​ Room temperature​  14 days​
​Refrigerate (preferred)  14 days​
​Frozen Indefinite
Rejection Criteria
If specimen is too viscous to pipette​​

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate

Stored in Transport Media, Fixative or Isolator Tubes
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Miravista Diagnostics Monday through Friday ​ 1 day​

Quantitative sandwich enzyme immunoassay (EIA)​

Serum is pre-treated to improve accuracy

Reference Lab
Test Information

Cross-reactions are seen with histoplasmosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidiooidomycosis, rarely in aspergillosis and possibly sporotrichosis.   Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay. 

Negative results do not exclude blastomycosis. Testing of both urine and serum offers the highest sensitivity. Failure of the antigen to rise does not exclude relapse.

The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

*New positives may require confirmation

Reference Range Information
​None Detected
Interpretation

Positive Below the Limit of Quantification: Results above the cutoff for positivity, but below 0.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable. Results will be confirmed and reported the next business day.

Positive: 0.2-14.7 ng/mL

Positive Above the Limit of Quantification: Results greater than 14.7 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87449
Synonyms/Keywords
​Blastomyces Dermatitidis QNT AG
Ordering Applications
Ordering Application Description
​Centricity ​Blastomyces Quant Ag (316)
​Cerner ​Blastomyces Quantitative Antigen, Serum (316)
​Portal​Blasto Quant Ag, Serum (316)
​COM​Blastomyces Quant Ag, Serum
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Minimum Volume
(allows for 1 repeat)
​Serum

​Red Top Tube (RTT)  

Serum Separator Tube (SST) ​1.2 mL
​Plasma​EDTA Lavender Top Tube (LTT)

​Sodium-heparin OR Lithium-heparin Green Top Tube (GTT)

OR

Sodium citrate tube (BTT)

​1.2 mL
Collection Processing

​Collect serum or plasma in a preferred or acceptable tube listed above.   Allow blood serum samples to clot for 30 minutes, then centrifuge. Pipette serum into a plastic leak-proof container.  Centrifuge blood plasma samples for 15 minutes and pipette plasma into a plastic leak-proof container.

​Ship to arrive Monday through Friday using a next day delivery service.  May be shipped ambient.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma ​ ​ Room temperature​  14 days​
​Refrigerate (preferred)  14 days​
​Frozen Indefinite
Rejection Criteria
If specimen is too viscous to pipette​​

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate

Stored in Transport Media, Fixative or Isolator Tubes
Useful For
​Aid in diagnosis of blastomycosis
Aid in monitoring to determine when treatment can be discontinued
Aid in diagnosis of relapse
Reference Range Information
​None Detected
Interpretation

Positive Below the Limit of Quantification: Results above the cutoff for positivity, but below 0.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable. Results will be confirmed and reported the next business day.

Positive: 0.2-14.7 ng/mL

Positive Above the Limit of Quantification: Results greater than 14.7 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Miravista Diagnostics Monday through Friday ​ 1 day​

Quantitative sandwich enzyme immunoassay (EIA)​

Serum is pre-treated to improve accuracy

Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87449
For most current information refer to the Marshfield Laboratory online reference manual.