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25014 Chronic Lymphocytic Leukemia (CLL), Diagnostic FISH (CLLDF)

Chronic Lymphocytic Leukemia (CLL), Diagnostic FISH (CLLDF)
Test Code: MISC
Synonyms/Keywords

​+12
11q- (11q deletion) or ATM
13q- (13q deletion) or LAMP1
17p- (17p deletion) or TP53
6q- (6q deletion) or MYB
t(11;14)(q13;q32) - CCND1/IGH

CLLFSO

Test Components

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

When an additional IGH signal is identified in the absence of a CCND1/IGH fusion, reflex testing using the IGHIBCL3 probe set will be performed to identify a potential IGH/BCL3 fusion [t(14;19)(q32;q13)]

Useful For
  • Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with chronic lymphocytic leukemia (CLL)
  • Identifying and tracking known chromosome abnormalities in patients with CLL and tracking response to therapy
  • Distinguishing patients with 11;14 translocations who have leukemic phase of mantle cell lymphoma from patients who have CLL
  • Detecting patients with atypical CLL or other forms of lymphoma associated with translocations between IGH and BCL3
Specimen Requirements

Specimen Type

Varies (submit only one)

 Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​ ​ Submit only 1 the following specimens:​
Whole Blood​ Sodium-Heparin Green Top Tube (GTT)  EDTA Lavender Top Tube (LTT)​ or ACD Yellow Top Tube 6 mL 2 mL​ ​
​Bone Marrow Sodium-Heparin Green Top Tube (GTT)  EDTA Lavender Top Tube (LTT)​ or ACD Yellow Top Tube 2 mL ​1 mL ​
Collection Processing Instructions

Necessary Information:

1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. A pathology and/or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.

Specimen Stability Information
Specimen Type Temperature
Varies​ ​ Ambient (preferred)​
​Refrigerated
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories​​
Monday through Friday
 7 to 10 days​
Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
Test Information
Additional probes may be added at an additional charge.​
Reference Range Information
Performing Location Reference Range
Mayo Clinic Laboratories​​ Interpretive report​
Interpretation

A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe set.

The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ 1 ​Interpretation and report ​
​​88271 ​ 12 DNA Probes
​​88275 ​ ​6 ​Interphases, 100-300
​88271​​2​DNA Probes​if needed
​88275​​1​Interphases, 100-300​if needed
Synonyms/Keywords

​+12
11q- (11q deletion) or ATM
13q- (13q deletion) or LAMP1
17p- (17p deletion) or TP53
6q- (6q deletion) or MYB
t(11;14)(q13;q32) - CCND1/IGH

CLLFSO

Test Components

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

When an additional IGH signal is identified in the absence of a CCND1/IGH fusion, reflex testing using the IGHIBCL3 probe set will be performed to identify a potential IGH/BCL3 fusion [t(14;19)(q32;q13)]

Ordering Applications
Ordering Application Description
​Cerner Chronic Lym Leuk,FISH (CLLDF)
​COM​Chronic Lymphocytic Leukemia (CLL), FISH (CLLDF)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements

Specimen Type

Varies (submit only one)

 Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​ ​ Submit only 1 the following specimens:​
Whole Blood​ Sodium-Heparin Green Top Tube (GTT)  EDTA Lavender Top Tube (LTT)​ or ACD Yellow Top Tube 6 mL 2 mL​ ​
​Bone Marrow Sodium-Heparin Green Top Tube (GTT)  EDTA Lavender Top Tube (LTT)​ or ACD Yellow Top Tube 2 mL ​1 mL ​
Collection Processing

Necessary Information:

1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. A pathology and/or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.

Specimen Stability Information
Specimen Type Temperature
Varies​ ​ Ambient (preferred)​
​Refrigerated
Useful For
  • Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with chronic lymphocytic leukemia (CLL)
  • Identifying and tracking known chromosome abnormalities in patients with CLL and tracking response to therapy
  • Distinguishing patients with 11;14 translocations who have leukemic phase of mantle cell lymphoma from patients who have CLL
  • Detecting patients with atypical CLL or other forms of lymphoma associated with translocations between IGH and BCL3
Test Components

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

When an additional IGH signal is identified in the absence of a CCND1/IGH fusion, reflex testing using the IGHIBCL3 probe set will be performed to identify a potential IGH/BCL3 fusion [t(14;19)(q32;q13)]

Reference Range Information
Performing Location Reference Range
Mayo Clinic Laboratories​​ Interpretive report​
Interpretation

A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe set.

The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories​​
Monday through Friday
 7 to 10 days​
Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88291 ​ 1 ​Interpretation and report ​
​​88271 ​ 12 DNA Probes
​​88275 ​ ​6 ​Interphases, 100-300
​88271​​2​DNA Probes​if needed
​88275​​1​Interphases, 100-300​if needed
For most current information refer to the Marshfield Laboratory online reference manual.