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25006 Oligoclonal Banding, CSF and Serum

Oligoclonal Banding, CSF and Serum
Test Code: OLIGO
Synonyms/Keywords
Oligoclonal Banding in CSF​
Useful For
This test is useful to help determine if increased CSF proteins are due to increased permeability of the blood - CSF barrier, or due to synthesis of immunoglobulins (primarily IgG) within the Central Nervous System.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​
CSF
and
Serum required
Spinal Fluid collection tube for CSF and
Red Top Tube (RTT) for Serum

Plastic tube for CSF​

Serum Separator Tube (SST) for Serum

1.0 mL CSF

1.0 mL Serum​

0.5 mL CSF

0.5 mL Serum​

0.5 mL CSF

0.5 mL Serum​

Collection Processing Instructions
Serum sample must be drawn within 24 hours of spinal fluid collection.
If serum is not collected within 24 hours of CSF collection, please call Customer Service to discuss the case with a Clinical Chemist.
Specimen Stability Information
Specimen Type Temperature Time
CSF/Serum​ ​ Refrigerate​ 7 days​
Frozen 1 month​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Thursday​ 6-8 hours​ Isoelectrofocusing with IgG detection/Sebia Hydrasys​
Test Information
This procedure involves isoelectrofocusing on an agarose gel to fractionate the proteins in the CSF and serum.  This is followed by immunofixation with anti-IgG antiserum.  The use of enzyme-labeled (peroxidase) anti-IgG antiserum permits detection of IgG oligoclonal banding at increased specificity and sensitivity.  The serum is needed to determine if oligoclonal bands are produced only in the Central Nervous System.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
83916​
Synonyms/Keywords
Oligoclonal Banding in CSF​
Ordering Applications
Ordering Application Description
​Centricity ​Oligoclonal Banding
​Cerner ​Oligoclonal Bands CSF and Serum
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​
CSF
and
Serum required
Spinal Fluid collection tube for CSF and
Red Top Tube (RTT) for Serum

Plastic tube for CSF​

Serum Separator Tube (SST) for Serum

1.0 mL CSF

1.0 mL Serum​

0.5 mL CSF

0.5 mL Serum​

0.5 mL CSF

0.5 mL Serum​

Collection Processing
Serum sample must be drawn within 24 hours of spinal fluid collection.
If serum is not collected within 24 hours of CSF collection, please call Customer Service to discuss the case with a Clinical Chemist.
Specimen Stability Information
Specimen Type Temperature Time
CSF/Serum​ ​ Refrigerate​ 7 days​
Frozen 1 month​
Useful For
This test is useful to help determine if increased CSF proteins are due to increased permeability of the blood - CSF barrier, or due to synthesis of immunoglobulins (primarily IgG) within the Central Nervous System.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Thursday​ 6-8 hours​ Isoelectrofocusing with IgG detection/Sebia Hydrasys​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
83916​
For most current information refer to the Marshfield Laboratory online reference manual.