PREFERRED: Obtain an adequate sampling from the anal-rectal canal using a cytobrush or Dacron swab. Lubricants should not be used prior to obtaining cytology sample, which may interfere with the processing and interpretation of the specimen. Insert the cytobrush or moistened Dacron swab into the anal canal until resistance is not met. Rotate the cytobrush or swab 360 degrees while gentle pressure is applied to the walls of the anal canal as the brush or swab is gradually withdrawn. Immediately rinse the cytobrush or swab in the ThinPrep PreServCyt Solution by rotating the collection device in the solution 10 times while pushing against the PreServCyt vial wall. Swirl the brush vigorously to further release material. Discard brush or swab. Tighten the cap so that the torque line on the cap passes the torque line on the vial. Record the patient name and medical record number on the vial and requisition form.