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24740 Cord Blood Testing

Cord Blood Testing
Test Code: CORD-B
Synonyms/Keywords
​Direct Antiglobulin Test, Direct Coombs, DAT, Coombs Test, ABO Grouping, Blood Typing, ABO-RH, Type and Rh
Test Components
​ABO/RH, DAT
Useful For
​Cord Blood Studies are performed on the cord blood of a newborn, when the mother is known Rh(o)D Negative, had an unknown Rh type, or has known significant antibody(ies). The ABO/RH test is performed to determine if a post partum Rh(o)D Negative mother is an Rh Immune Globulin candidate. The DAT is performed to determine if maternal antibody is coating the newborn red cells.  When the DAT is positive, elution studies will be performed to determine the maternal antibody(ies) that are coating the cord cells.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No Cord blood​ 6 mL EDTA Pink Top Tube (PTT)
3 mL EDTA Lavender Top Tubes (LTT)​
6 mL​ 3 mL​
If Cord blood was not collected,
Two 0.5 mL microcollection tubes
Collection Processing Instructions

​Cord blood specimens for blood bank studies must be labeled with baby's first and last name, medical record number, collection date and time, and collector's initials.

DO NOT label the cord blood sample with mother's name or medical record number.

Specimen Stability Information
Specimen Type Temperature Time
​Cord blood Refrigerate​ <48 hours old​
Rejection Criteria
>48 hrs old
Plasma/Serum must not be separated from the red cells​
Frozen​
Hemolyzed​
Serum separator tube (SST)
Interference
​Wharton’s jelly may contaminate the specimen
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ Less than 2 hours​ Serological​
Test Information

​Cord Blood Studies are performed on the cord blood of a newborn, when the mother is known Rh(o)D Negative, had an unknown Rh type, or has known significant antibody(ies). The ABO/RH test is performed to determine if a post partum Rh(o)D Negative mother is an Rh Immune Globulin candidate. The DAT is performed to determine if maternal antibody is coating the newborn red cells. Cord Blood specimens for Transfusion Services studies must be labeled with Baby's first and last name, Medical Record number, Collection Date and Time and collector's initials. Do not label the Cord Blood Sample with Mother's name or medical record number. Order Cord Blood Study in the Hospital computer system. Fill out a Hospital Blood Bank Test Request for the Cord Blood Study with baby's Name, Medical Record Number, Admit number, requesting physician, and mark ABO/RH and DAT in the serological procedures column. If mother is a possible Rh Immune Globulin candidate, fill out a second Hospital Blood Bank Test Request with Mother's Name, Medical Record Number, Admit number, requesting physician, and mark FBSC (Fetal blood screen) in the serological procedures column. Send the properly labeled specimen, Cord Blood order slip, and Hospital blood bank test request(s) to blood bank.

Cord Blood studies include Blood Group and Type (ABO/RH), and Direct Antiglobulin test (DAT). Specimen is tested with polyspecific antiserum. If positive, monospecific IgG and C3 will be tested and charged. When the DAT is positive, elution studies will be performed to determine the maternal antibody(ies) that are coating the cord cells.

Reference Range Information
Performing Location Reference Range
​Marshfield DAT Negative ​
Interpretation
​See Critical Value List for list of current critical values
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86880 ​ DAT​
86900 ​ ABO​
86901 ​ RH​
Synonyms/Keywords
​Direct Antiglobulin Test, Direct Coombs, DAT, Coombs Test, ABO Grouping, Blood Typing, ABO-RH, Type and Rh
Test Components
​ABO/RH, DAT
Ordering Applications
Ordering Application Description
​Cerner​Cord Blood Type and DAT
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No Cord blood​ 6 mL EDTA Pink Top Tube (PTT)
3 mL EDTA Lavender Top Tubes (LTT)​
6 mL​ 3 mL​
If Cord blood was not collected,
Two 0.5 mL microcollection tubes
Collection Processing

​Cord blood specimens for blood bank studies must be labeled with baby's first and last name, medical record number, collection date and time, and collector's initials.

DO NOT label the cord blood sample with mother's name or medical record number.

Specimen Stability Information
Specimen Type Temperature Time
​Cord blood Refrigerate​ <48 hours old​
Rejection Criteria
>48 hrs old
Plasma/Serum must not be separated from the red cells​
Frozen​
Hemolyzed​
Serum separator tube (SST)
Interference
​Wharton’s jelly may contaminate the specimen
Useful For
​Cord Blood Studies are performed on the cord blood of a newborn, when the mother is known Rh(o)D Negative, had an unknown Rh type, or has known significant antibody(ies). The ABO/RH test is performed to determine if a post partum Rh(o)D Negative mother is an Rh Immune Globulin candidate. The DAT is performed to determine if maternal antibody is coating the newborn red cells.  When the DAT is positive, elution studies will be performed to determine the maternal antibody(ies) that are coating the cord cells.
Test Components
​ABO/RH, DAT
Reference Range Information
Performing Location Reference Range
​Marshfield DAT Negative ​
Interpretation
​See Critical Value List for list of current critical values
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ Less than 2 hours​ Serological​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86880 ​ DAT​
86900 ​ ABO​
86901 ​ RH​
For most current information refer to the Marshfield Laboratory online reference manual.