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23392 Parvovirus B19, DNA Detection by Rapid PCR (PARVO)

Parvovirus B19, DNA Detection by Rapid PCR (PARVO)
Test Code: MISC
Synonyms/Keywords
B19, Erythrovirus B19, Fifth Disease-Parvovirus, Human Parvovirus B19, Parvovirus B19 PCR, Parvovirus PCR, Parvovirus-Fifth Disease
Useful For
Diagnosing parvovirus B19 infection
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Specimen source is required.
Submit only 1 of the following specimens:
Amniotic fluid (preferred)​ ​Amniotic fluid container ​0.5 mL 0.3 mL​
​Spinal fluid (preferred) ​Sterile vial ​0.5 mL ​0.3 mL
​Synovial fluid (preferred) ​Sterile vial or EDTA Lavender Top Tube (LTT) ​0.5 mL ​0.3 mL
Bone marrow (alternate)​ Sterile container or EDTA Lavender Top Tube (LTT)​ ​0.5 mL ​0.3 mL
Collection Processing Instructions

​Label the specimen specifically with the source.

Do not centrifuge amniotic fluid or spinal fluid. 

Specimen Stability Information
Specimen Type Temperature Time
Varies​ ​ ​Refrigerated (preferred) ​7 days
​Frozen ​7 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ 1 day
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
Test Information

Parvovirus B19 is a DNA virus that preferentially replicates in erythroid progenitor cells. Infection with parvovirus B19 can occur at any age, but is most common early in life. Antibody prevalence ranges from 2% to 15% in children 1 to 5 years old to 30% to 60% in adults. The virus is transmitted by respiratory secretions and occasionally by blood products.

Parvovirus B19 infections can be asymptomatic or produce a wide spectrum of disease ranging from erythema infectiosum ("fifth disease" characterized by a classic "slapped cheek" rash) in children to arthropathy, severe anemia, and systemic manifestations involving the central nervous system, heart, and liver depending on the immune competence of the host. Infection with parvovirus B19 in pregnant women may cause hydrops fetalis, congenital anemia, spontaneous abortion, or stillbirth of the fetus.Parvovirus B19 is also the causative agent of transient aplastic crisis and chronic aplasia usually, but not exclusively, in immunocompromised or transplant patients, and those with preexisting hematologic disorders (eg, sickle cell disease).

Most acute infections with parvovirus B19 are diagnosed in the laboratory by serologically detecting IgG- and IgM-class antibodies with enzyme-linked immunosorbent assay testing.

Reference Range Information
Negative
Interpretation

A positive result indicates that parvovirus B19 DNA is present in the clinical sample. However, a positive result does not differentiate between actively replicating virus, transient infection that may be asymptomatic, or the presence of remnant viral nucleic acid.

A negative result suggests the absence of parvovirus B19 infection.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87798
Synonyms/Keywords
B19, Erythrovirus B19, Fifth Disease-Parvovirus, Human Parvovirus B19, Parvovirus B19 PCR, Parvovirus PCR, Parvovirus-Fifth Disease
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Specimen source is required.
Submit only 1 of the following specimens:
Amniotic fluid (preferred)​ ​Amniotic fluid container ​0.5 mL 0.3 mL​
​Spinal fluid (preferred) ​Sterile vial ​0.5 mL ​0.3 mL
​Synovial fluid (preferred) ​Sterile vial or EDTA Lavender Top Tube (LTT) ​0.5 mL ​0.3 mL
Bone marrow (alternate)​ Sterile container or EDTA Lavender Top Tube (LTT)​ ​0.5 mL ​0.3 mL
Collection Processing

​Label the specimen specifically with the source.

Do not centrifuge amniotic fluid or spinal fluid. 

Specimen Stability Information
Specimen Type Temperature Time
Varies​ ​ ​Refrigerated (preferred) ​7 days
​Frozen ​7 days
Useful For
Diagnosing parvovirus B19 infection
Reference Range Information
Negative
Interpretation

A positive result indicates that parvovirus B19 DNA is present in the clinical sample. However, a positive result does not differentiate between actively replicating virus, transient infection that may be asymptomatic, or the presence of remnant viral nucleic acid.

A negative result suggests the absence of parvovirus B19 infection.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ 1 day
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87798
For most current information refer to the Marshfield Laboratory online reference manual.