Skip Ribbon Commands
Skip to main content
Sign In
Navigate Up

23243 Maternal Serum Screen Quadruple Panel

Maternal Serum Screen Quadruple Panel
Test Code: AFP-4
Synonyms/Keywords
AFP4, Alpha Fetoprotein-4, AFP-4, Quad Screen​
Test Components
Alpha Fetoprotein, Inhibin A, Estriol, Human Chorionic Gonadotropin​
Useful For
Useful for prenatal screening of open neural tube defect (ONTD), Down syndrome and trisomy 18.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)​ Red Top Tube (RTT)​2.0 mL​0.5 mL​0.275 mL​
Collection Processing Instructions
Sample must be drawn between 15 weeks/0 day and 21 weeks/6 days gestation. Samples collected in a RTT must be removed from the cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube. ​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated​ 5 days​
Frozen​ >5 days​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​1 day​ Two-site Immunoenzymatic Assay (Beckman Coulter DXI) ​
Test Information
A completed Marshfield Labs Maternal Serum Request Requisition Form must accompany the specimen or the information must be entered during order entry in Clinical Order Manager. These forms can be obtained calling 800-222-5835. The abnormal reports are faxed to the Provider by Marshfield Clinic Medical Genetics staff.
 
 
It is recommended that women who have the first trimester screening for aneuploidy should not undergo second trimester quadruple screening. If first and second trimester screens are performed for the same disorder, the false positive rates are additive and therefore, can lead to unnecessary invasive procedures. However, if women who have first trimester screening performed for aneuploidy and wish to assess their risk for an open neural tube defect, the AFP-1 test should be ordered.
 
Reference Range Information
Performing Location Reference Range
Marshfield​ See interpretive report​
Interpretation

NEURAL TUBE DEFECTS
An AFP MoM < 2.5 is reported as Screen-negative.
An AFP MoM of >= 2.5 (singleton pregnancy) is reported as Screen-positive.

DOWN SYNDROME
An estimated screen risk < 1/300 is reported as Screen-negative.
An estimated screen risk >= 1/300 is reported as Screen-positive.

TRISOMY 18
An estimated screen risk < 1/100 is reported as Screen-negative.
An estimated screen risk >= 1/100 is reported as Screen-positive.

A Screen-negative result indicates that the calculated screen risk falls below the established cut-off for the open NTDs, Down syndrome and trisomy 18. A negative screen does not guarantee the absence of fetal defect. A Screen-positive result indicates an increased risk for open NTDs, Down syndrome and trisomy 18. It does not infer a definitive diagnosis, but indicates that further evaluation should be considered. ​

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82105​ ​ ​ AFP​ ​
82677​ ​ ​ Estriol​ ​
84702​ ​ ​ hCG​ ​
86336 ​ ​ ​ Inhibin A​ ​
Synonyms/Keywords
AFP4, Alpha Fetoprotein-4, AFP-4, Quad Screen​
Test Components
Alpha Fetoprotein, Inhibin A, Estriol, Human Chorionic Gonadotropin​
Ordering Applications
Ordering Application Description
​Centricity ​AFP-4 (Quadruple MSS)
​Cerner ​AFP-4 Test Panel (Quad Screen)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)​ Red Top Tube (RTT)​2.0 mL​0.5 mL​0.275 mL​
Collection Processing
Sample must be drawn between 15 weeks/0 day and 21 weeks/6 days gestation. Samples collected in a RTT must be removed from the cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube. ​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated​ 5 days​
Frozen​ >5 days​
Useful For
Useful for prenatal screening of open neural tube defect (ONTD), Down syndrome and trisomy 18.​
Test Components
Alpha Fetoprotein, Inhibin A, Estriol, Human Chorionic Gonadotropin​
Reference Range Information
Performing Location Reference Range
Marshfield​ See interpretive report​
Interpretation

NEURAL TUBE DEFECTS
An AFP MoM < 2.5 is reported as Screen-negative.
An AFP MoM of >= 2.5 (singleton pregnancy) is reported as Screen-positive.

DOWN SYNDROME
An estimated screen risk < 1/300 is reported as Screen-negative.
An estimated screen risk >= 1/300 is reported as Screen-positive.

TRISOMY 18
An estimated screen risk < 1/100 is reported as Screen-negative.
An estimated screen risk >= 1/100 is reported as Screen-positive.

A Screen-negative result indicates that the calculated screen risk falls below the established cut-off for the open NTDs, Down syndrome and trisomy 18. A negative screen does not guarantee the absence of fetal defect. A Screen-positive result indicates an increased risk for open NTDs, Down syndrome and trisomy 18. It does not infer a definitive diagnosis, but indicates that further evaluation should be considered. ​

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​1 day​ Two-site Immunoenzymatic Assay (Beckman Coulter DXI) ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82105​ ​ ​ AFP​ ​
82677​ ​ ​ Estriol​ ​
84702​ ​ ​ hCG​ ​
86336 ​ ​ ​ Inhibin A​ ​
For most current information refer to the Marshfield Laboratory online reference manual.