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23227 C3a Level By RIA (FC3AR)

C3a Level By RIA (FC3AR)
Test Code: C3ASO
Synonyms/Keywords

​​C3 anaphylatoxin, C3a des Arg, C3a Level, Complement C3a level

C3a Level (FC3AR)

Specimen Requirements
Specimen Type Preferred Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Plasma 
 ​EDTA Lavender Top Tube (LTT) ​1.5 mL ​0.5 mL
Collection Processing Instructions
Draw blood in a lavender-top (EDTA) tube(s). No alternate specimen is acceptable.  Plasma gel tube(s) is not acceptable   
Mix well. 
Within 30 minutes of collection, centrifuge at 1600 (+/-200) x g(rcf) at 4 degrees C for 15 minutes.
Freeze immediately at -20 degrees C for up to 4 weeks or, ideally, at -60 degrees C or below for up to 1 year.
Send frozen

​Complete and submit with specimen:  National Jewish Complement request form.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma EDTA ​Frozen ​365 days
Rejection Criteria

Thawed specimens

Specimens collected in Plasma Gel Tube/PST

Gross hemolysis

Interference

​Elevated C3a levels are indicative of classical, alternative and/or lectin pathway activation.​

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available
 Methodology/Instrumentation
​Mayo Medical Laboratories
 forwards to National Jewish Health		 Monday through Friday
 ​21 to 44 days
Radioimmunoassay (RIA)​
Reference Lab
Mayo Clinic Laboratories
Test Information

This test should be regarded as 'Research Use Only'. This test has not been cleared or approved by the U.S. Food and Drug Administration. It may not be covered by insurance and providers need to inform all patients of this prior to ordering. 

For Medicare patients, an Advanced Beneficiary notice (ABN) is required; for Medicaid patients, a Noncovered Services Waiver is required, and for commercial payers, prior authorization should be obtained.   ​

Test is forwarded from Mayo to National Jewish Reference Laboratories. Performance characteristics have been validated by National Jewish Health.

The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.​

Reference Range Information
0-780 ng/mL
Outreach CPTs
 
CPT Modifier
(if needed)		 Quantity Description Comments
​86160 ​​1 ​ ​
Classification

This test should be regarded as 'Research Use Only'. This test has not been cleared or approved by the U.S. Food and Drug Administration. It may not be covered by insurance and providers need to inform all patients of this prior to ordering. 

For Medicare patients, an Advanced Beneficiary notice (ABN) is required; for Medicaid patients, a Noncovered Services Waiver is required, and for commercial payers, prior authorization should be obtained.    

Synonyms/Keywords

​​C3 anaphylatoxin, C3a des Arg, C3a Level, Complement C3a level

C3a Level (FC3AR)

Ordering Applications
Ordering Application Description
​​Cerner
 C3a Level (FC3AR)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Plasma 
 ​EDTA Lavender Top Tube (LTT) ​1.5 mL ​0.5 mL
Collection Processing
Draw blood in a lavender-top (EDTA) tube(s). No alternate specimen is acceptable.  Plasma gel tube(s) is not acceptable   
Mix well. 
Within 30 minutes of collection, centrifuge at 1600 (+/-200) x g(rcf) at 4 degrees C for 15 minutes.
Freeze immediately at -20 degrees C for up to 4 weeks or, ideally, at -60 degrees C or below for up to 1 year.
Send frozen

​Complete and submit with specimen:  National Jewish Complement request form.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma EDTA ​Frozen ​365 days
Rejection Criteria

Thawed specimens

Specimens collected in Plasma Gel Tube/PST

Gross hemolysis

Interference

​Elevated C3a levels are indicative of classical, alternative and/or lectin pathway activation.​

Reference Range Information
0-780 ng/mL
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available
 Methodology/Instrumentation
​Mayo Medical Laboratories
 forwards to National Jewish Health		 Monday through Friday
 ​21 to 44 days
Radioimmunoassay (RIA)​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
 
CPT Modifier
(if needed)		 Quantity Description Comments
​86160 ​​1 ​ ​
Classification

This test should be regarded as 'Research Use Only'. This test has not been cleared or approved by the U.S. Food and Drug Administration. It may not be covered by insurance and providers need to inform all patients of this prior to ordering. 

For Medicare patients, an Advanced Beneficiary notice (ABN) is required; for Medicaid patients, a Noncovered Services Waiver is required, and for commercial payers, prior authorization should be obtained.    

For most current information refer to the Marshfield Laboratory online reference manual.