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23148 Culture, Organism Susceptibility Only, Aerobic

Culture, Organism Susceptibility Only, Aerobic
Test Code: SENS
Synonyms/Keywords
​Culture Sensi, Aerobic
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Pure culture of aerobic bacterium Sealed agar plates

Agar slant (e.g. chocolate, tryptic soy)

 

 
Broth (e.g. tryptic soy, thioglycollate)

 

Collection Processing Instructions

Submit pure culture of organism on agar plates to keep turn-around-time of susceptibility to a minimum.

Clearly state the name (ID) of the organism submitted for susceptibility testing. If the identification is not indicated, the organism will be identified at an additional charge. If the organism identification is unknown, order the IDEN test and indicate that susceptibility testing is desired. 

Do not send primary plates or plates with mixed cultures.  Multiple organisms submitted will be tested and charged separately. Delay in results will occur.
 
Sensitivity CPT and fee depend upon methodology.
 
Ensure that plates are properly sealed (e.g. tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
Specimen Type Temperature Time
Agar plate or slant​ After incubation, store at room temperature​ 5 days​
Broth​ Room temperature​ 7 days​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 3 days​ Manual or automated standardized susceptibility testing​
Test Information
​If testing of specific antimicrobial agents is desired, please indicate that on the test request form. If not indicated, standard panels will be tested.  
Antimicrobial Susceptibility Testing (AST) will be performed if standardized methods are available based upon Clinical Laboratory Standards Institute guidelines. Methods used include disk diffusion, minimum inhibitory concentration (MIC) by broth microdilution, or MIC by Etest®.
A listing of the tested and reported antimicrobial agents can be seen at Antimicrobial Susceptibility Panels. If testing against a specific antimicrobial agent is desired, contact the microbiology laboratory.
 
The microbiology laboratory uses selective reporting of susceptibility results in order to promote rational antimicrobial usage. For more information on selective reporting, please contact the microbiology technical manager. 
 
Standardized AST is not available for all bacterial species, in which case an MIC determined by a non-standardized method and without interpretation may be available upon request. 
 
The microbiology laboratory publishes an annual cumulative antibiogram for commonly isolated bacteria, available at  Marshfield Medical Center (Marshfield, WI), Cumulative Antibiogram Reports. Printed copies are also available from the microbiology laboratory.
 
‘E-test’ is a registered trademark of bioMerieux Inc.
Reference Range Information
Performing Location Reference Range
​Marshfield Interpretive report​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87186​ ​1 MIC susceptibility, micro-broth dilution ​As needed
87184​ ​1 ​KB (disk) susceptbility ​As needed
​87181 ​1 ​MIC susceptibility, Etest® strip gradient ​As needed
​87147 ​1 ​Typing, Agglutanation ​As needed
Synonyms/Keywords
​Culture Sensi, Aerobic
Ordering Applications
Ordering Application Description
​Centricity ​CSF Culture Sensitivity
​Cerner None​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Pure culture of aerobic bacterium Sealed agar plates

Agar slant (e.g. chocolate, tryptic soy)

 

 
Broth (e.g. tryptic soy, thioglycollate)

 

Collection Processing

Submit pure culture of organism on agar plates to keep turn-around-time of susceptibility to a minimum.

Clearly state the name (ID) of the organism submitted for susceptibility testing. If the identification is not indicated, the organism will be identified at an additional charge. If the organism identification is unknown, order the IDEN test and indicate that susceptibility testing is desired. 

Do not send primary plates or plates with mixed cultures.  Multiple organisms submitted will be tested and charged separately. Delay in results will occur.
 
Sensitivity CPT and fee depend upon methodology.
 
Ensure that plates are properly sealed (e.g. tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
Specimen Type Temperature Time
Agar plate or slant​ After incubation, store at room temperature​ 5 days​
Broth​ Room temperature​ 7 days​
Reference Range Information
Performing Location Reference Range
​Marshfield Interpretive report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 3 days​ Manual or automated standardized susceptibility testing​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87186​ ​1 MIC susceptibility, micro-broth dilution ​As needed
87184​ ​1 ​KB (disk) susceptbility ​As needed
​87181 ​1 ​MIC susceptibility, Etest® strip gradient ​As needed
​87147 ​1 ​Typing, Agglutanation ​As needed
For most current information refer to the Marshfield Laboratory online reference manual.