Skip Ribbon Commands
Skip to main content
Navigate Up
Search
Test Code
Search
Laboratory Section
Test Category
Browse By Test Name
# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
HIV-1,2 Antibody EIA with Confirm Eval
Test Code: HIVR
Synonyms/Keywords
 HIV-1,2 Ab EIA with Confirmation
Test Components

HIV 1 and 2 Antibody, HIV Index

Useful For
Used for the detection of antibodies to the human Immunodeficiency virus type 1, including Group O, and/or type 2 in serum.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​  Serum​ Red Top Tube (RTT) Serum Separator (SST)​ 1 mL​ 0.5 mL​
Collection Processing Instructions
Separate serum from the blood within 60 minutes of venipuncture. Recommended guidelines for optimal sample processing should be followed. Specimen must be free of particulate matter including fibrin. Specimens transported to Marshfield must be removed from the primary collection tube and sent in a labeled daughter tube. Store samples at 2 to 8°C if not tested within 24 hours of collection. The frozen specimens should be completely thawed thoroughly mixed and centrifuged before analysis. 6 freeze/thaw cycles has shown to have no qualitative difference in results.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​ Ambient​ 24 hours​
Refrigerate​ 14 days​
Frozen​ (-20C) 168 days​
Rejection Criteria
Gross hemolysis & icterus specimens. No interference with gross lipemia has been recognized. Specimen will be rejected if contaminated with microbes, or containing precipitate. Cadaver specimens, heat-inactivated specimens, cord blood, neonatal specimens or body fluids other than serum such as saliva, urine, amniotic, or pleural fluids.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday​ 1 day​ Sandwich Chemiluminometric Immunoassay/Centaur, Siemens
Test Information

The consenting process changed in July 2010; therefore consent form is not required.

This test uses a 3-tier algorithm: HIV Algorithm

All three components are performed at the Marshfield center, Marshfield Clinic.

Reference Range Information
Performing Location Reference Range
Marshfield​ ​ Expected Value: Non-Reactive
​Results are reported as "NON-REACT" or "REACTIVE"
Interpretation
Western blot analysis is no longer recommended nor used to confirm HIV EIA antibody-positive results. The 3-tier algorithm described above will yield unambiguous results for HIV status in virtually all cases. Tier 1, and tier 2 when indicated, will be performed automatically; tier 3, the HIV-1 Viral Load assay (test code: HIVPCR) will be recommended in consultation with the ordering provider. Note that the vast majority of samples will yield a definitive result of HIV status by the end of tier 2.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86703 ​ ​1 HIV-1,2 Antibody EIA
86701 ​1 HIV-1 Antibody (confirmation) ​If needed
86702  ​1 HIV-2 Antibody (confirmation) ​If needed
Synonyms/Keywords
 HIV-1,2 Ab EIA with Confirmation
Test Components

HIV 1 and 2 Antibody, HIV Index

Ordering Applications
Ordering Application Description
​Centricity ​HIV-1,2 Ab EIA w/Confirm Eval
​Cerner ​HIV 1,2 Antibody With Confirmation (Common)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​  Serum​ Red Top Tube (RTT) Serum Separator (SST)​ 1 mL​ 0.5 mL​
Collection Processing Instructions
Separate serum from the blood within 60 minutes of venipuncture. Recommended guidelines for optimal sample processing should be followed. Specimen must be free of particulate matter including fibrin. Specimens transported to Marshfield must be removed from the primary collection tube and sent in a labeled daughter tube. Store samples at 2 to 8°C if not tested within 24 hours of collection. The frozen specimens should be completely thawed thoroughly mixed and centrifuged before analysis. 6 freeze/thaw cycles has shown to have no qualitative difference in results.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​ Ambient​ 24 hours​
Refrigerate​ 14 days​
Frozen​ (-20C) 168 days​
Rejection Criteria
Gross hemolysis & icterus specimens. No interference with gross lipemia has been recognized. Specimen will be rejected if contaminated with microbes, or containing precipitate. Cadaver specimens, heat-inactivated specimens, cord blood, neonatal specimens or body fluids other than serum such as saliva, urine, amniotic, or pleural fluids.
Useful For
Used for the detection of antibodies to the human Immunodeficiency virus type 1, including Group O, and/or type 2 in serum.
Test Information

The consenting process changed in July 2010; therefore consent form is not required.

This test uses a 3-tier algorithm: HIV Algorithm

All three components are performed at the Marshfield center, Marshfield Clinic.

Reference Range Information
Performing Location Reference Range
Marshfield​ ​ Expected Value: Non-Reactive
​Results are reported as "NON-REACT" or "REACTIVE"
Interpretation
Western blot analysis is no longer recommended nor used to confirm HIV EIA antibody-positive results. The 3-tier algorithm described above will yield unambiguous results for HIV status in virtually all cases. Tier 1, and tier 2 when indicated, will be performed automatically; tier 3, the HIV-1 Viral Load assay (test code: HIVPCR) will be recommended in consultation with the ordering provider. Note that the vast majority of samples will yield a definitive result of HIV status by the end of tier 2.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday​ 1 day​ Sandwich Chemiluminometric Immunoassay/Centaur, Siemens
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86703 ​ ​1 HIV-1,2 Antibody EIA
86701 ​1 HIV-1 Antibody (confirmation) ​If needed
86702  ​1 HIV-2 Antibody (confirmation) ​If needed
For most current information refer to the Marshfield Laboratory online reference manual.