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23120 HIV-1,2 Ab/Ag by EIA with Confirmation

HIV-1,2 Ab/Ag by EIA with Confirmation
Test Code: HIVR
Synonyms/Keywords
HIV-1 Antibody, HIV-2 Antibody, HIV-1 p24 Antigen, HIV-1, HIV-2
Test Components
Single qualitative result for reactivity of HIV-1,2 Antibody/Antigen, with automatic reflex to confirmatory testing.
Useful For
Used for the detection of antibodies to the human Immunodeficiency virus type 1, including Group O, and/or type 2 in serum.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ EDTA Plasma EDTA Pink Top Tube (PTT) EDTA Lavender Top Tube (LTT)
 1 mL​ 0.5 mL​ ​

​

​​Serum (Screen only, see Note below)
​​Red Top Tube (RTT) or Serum Separator Tube (SST)
​1 mL
​0.5 mL
​
Collection Processing Instructions
·       Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either sample type.
·       EDTA plasma samples may be centrifuged immediately after collection.
·       If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.
·       After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.
·       Specimens must be free of particulate matter including fibrin.
 
            Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation  
                     testing of REACTIVE samples.
 
            If REACTIVE, Mayo Medical Labs will require an additional 1 mL of EDTA plasma.
Specimen Stability Information
Specimen Type Temperature Time
Plasma / Serum
 Ambient​
3 days
Refrigerate​ 7 days​
30 days
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Sunday​ 2 hours Immunoenzymatic assay/Beckman Coulter DXI 9000​
Test Information

The consenting process changed in July 2010; therefore consent form is not required.

This test uses a 3-tier algorithm: HIV Algorithm 

Western blot analysis is no longer recommended nor used to confirm HIV EIA antibody-positive results. The 3-tier algorithm described above will yield unambiguous results for HIV status in virtually all cases. Tier 1 (HIV-1/2 Ag/Ab combination immunoassay) will be performed at Marshfield; tier 2 (HIV-1/HIV-2 Ab differentiation immunoassay) will be automatically reflexed and sent to an appropriate testing laboratory; tier 3, the HIV-1 Viral Load assay (test code: HIVPCR) will be recommended in consultation with the ordering provider. Note that the vast majority of samples will yield a definitive result of HIV status by the end of tier 2.
Reference Range Information
Performing Location Reference Range
Marshfield​ ​ Expected Value: Non-Reactive
​Results are reported as "Non-reactive" or "Reactive​"
Interpretation

NON-REACTIVE: This screening test found no evidence of HIV-1 or HIV-2 antibodies or HIV-1 p24 Antigen.

REACTIVE: This screening test is REACTIVE for HIV-1 and/or HIV-2 antibodies or HIV-1 p24 Antigen. An HIV-1 and HIV-2 Antibody Confirmation and Differentiation assay is automatically ordered.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87389 ​ ​ ​1 HIV-1 Ag(s), with HIV-1 and HIV-2 Abs, single result ​
Synonyms/Keywords
HIV-1 Antibody, HIV-2 Antibody, HIV-1 p24 Antigen, HIV-1, HIV-2
Test Components
Single qualitative result for reactivity of HIV-1,2 Antibody/Antigen, with automatic reflex to confirmatory testing.
Ordering Applications
Ordering Application Description
​Centricity ​HIV-1,2 Ab/Ag EIA w/Confirm
​Cerner ​HIV 1,2 Antibody/Antigen w/Confirmation (Common)
​Clinical Order Manager ​​HIV-1,2 Ab/Ag EIA w/Confirm
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ EDTA Plasma EDTA Pink Top Tube (PTT) EDTA Lavender Top Tube (LTT)
 1 mL​ 0.5 mL​ ​

​

​​Serum (Screen only, see Note below)
​​Red Top Tube (RTT) or Serum Separator Tube (SST)
​1 mL
​0.5 mL
​
Collection Processing
·       Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either sample type.
·       EDTA plasma samples may be centrifuged immediately after collection.
·       If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.
·       After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.
·       Specimens must be free of particulate matter including fibrin.
 
            Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation  
                     testing of REACTIVE samples.
 
            If REACTIVE, Mayo Medical Labs will require an additional 1 mL of EDTA plasma.
Specimen Stability Information
Specimen Type Temperature Time
Plasma / Serum
 Ambient​
3 days
Refrigerate​ 7 days​
30 days
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
Useful For
Used for the detection of antibodies to the human Immunodeficiency virus type 1, including Group O, and/or type 2 in serum.
Test Components
Single qualitative result for reactivity of HIV-1,2 Antibody/Antigen, with automatic reflex to confirmatory testing.
Reference Range Information
Performing Location Reference Range
Marshfield​ ​ Expected Value: Non-Reactive
​Results are reported as "Non-reactive" or "Reactive​"
Interpretation

NON-REACTIVE: This screening test found no evidence of HIV-1 or HIV-2 antibodies or HIV-1 p24 Antigen.

REACTIVE: This screening test is REACTIVE for HIV-1 and/or HIV-2 antibodies or HIV-1 p24 Antigen. An HIV-1 and HIV-2 Antibody Confirmation and Differentiation assay is automatically ordered.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Sunday​ 2 hours Immunoenzymatic assay/Beckman Coulter DXI 9000​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87389 ​ ​ ​1 HIV-1 Ag(s), with HIV-1 and HIV-2 Abs, single result ​
For most current information refer to the Marshfield Laboratory online reference manual.