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23096 Tryptase, Serum (TRYPT)

Tryptase, Serum (TRYPT)
Test Code: TRYPT
Synonyms/Keywords
Mast Cell Tryptase
Useful For
Assessing mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
 
Assessing patients with systemic mastocytosis or mast cell activation syndrome
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​0.5 mL ​0.2 mL
Collection Processing Instructions
​Send specimen in a plastic vial
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Frozen (preferred) ​14 days
​Refrigerated 7 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​1 day
Fluorescence Enzyme Immunoassay (FEIA)​
Reference Lab
Test Information
Tryptase, a neutral protease, is a dominant protein component of the secretory granules of human mast cells. There are 2 forms of tryptase, designated as alpha and beta, which are encoded by 2 separate genes. Both are expressed as inactive proenzymes. Alpha-protryptase and beta-protryptase are spontaneously released from resting mast cells. The levels of the protrypases reflect the total number of mast cells within the body, but are not an indication of mast cell activation. Beta-protryptase is processed to a mature form, which is stored in granules and released as an active tetramer that is bound to heparin or chondroitin sulfate proteoglycans. In contrast, an amino acid change in alpha-protryptase prevents processing to a mature form. Upon mast cell activation, degranulation releases mature tryptase, which is almost exclusively in the form of beta-tryptase.
 
After anaphylaxis, mast cell granules release tryptase; measurable amounts are found in blood, generally within 30 to 60 minutes. The levels decline under first-order kinetics with half-life of approximately 2 hours. By comparison, histamine (another immunologic mediator released by activated mast cells) is cleared from blood within minutes. Increased serum levels may also occur after allergen challenge or in patients with systemic mastocytosis or mast cell activation syndrome.
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories
<11.5 ng/mL
Interpretation
Levels of total tryptase in serum > or =11.5 ng/mL may indicate mast cell activation occurring as a result of anaphylaxis or allergen challenge, or it may indicate increased number of mast cells as seen in patients with mastocytosis.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83520
Synonyms/Keywords
Mast Cell Tryptase
Ordering Applications
Ordering Application Description
​Centricity ​Tryptase
​Cerner ​Tryptase (81608)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​0.5 mL ​0.2 mL
Collection Processing
​Send specimen in a plastic vial
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Frozen (preferred) ​14 days
​Refrigerated 7 days
Useful For
Assessing mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
 
Assessing patients with systemic mastocytosis or mast cell activation syndrome
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories
<11.5 ng/mL
Interpretation
Levels of total tryptase in serum > or =11.5 ng/mL may indicate mast cell activation occurring as a result of anaphylaxis or allergen challenge, or it may indicate increased number of mast cells as seen in patients with mastocytosis.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​1 day
Fluorescence Enzyme Immunoassay (FEIA)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83520
For most current information refer to the Marshfield Laboratory online reference manual.