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23058 Surgical Pathology, HPV DNA ISH (HPVHL)

Surgical Pathology, HPV DNA ISH (HPVHL)
Test Code: HPVDNSO
Synonyms/Keywords

HPV (Human Papillomavirus)

HPVHR

Papillomavirus

GPHPVHLISH
 
ISHPV611ISH
 
ISHPVAM16ISH
 
ISNEGHPVISH
 
ISDNAHPVISH
 
Test Components

Human papillomavirus (HPV) High-Risk E6/E7 RNA will be performed at the discretion of the Mayo pathologist.

Useful For
Detection of both low-risk (6, 11) and high-risk genotypes (16, 18, 31, 33, and 51) of human papillomavirus DNA in paraffin-embedded human tissue.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Formalin-fixed, paraffin-embedded tissue block ​Entire block
​6 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue ​6 slides
Collection Processing Instructions
A pathology/diagnostic report and a brief history are required.
Acceptable Specimen Types
​10% neutral buffered formalin fixed tissue specimen/ paraffin embedded tissue block
Specimen Stability Information
Specimen Type Temperature Time
​Tissue Slides Ambient ​ Indefinite
​10% Neutral Buffered Formalin Fixed Tissue Specimen ​ Ambient (preferred) ​ 6 months
​Refrigerate ​1 month
Rejection Criteria
Wet/Frozen Tissue
​Cytology Smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday - Friday ​5 days In Situ Hybridization (ISH)
Reference Lab
Test Information

​Human papillomavirus (HPV) infections with low-risk genotypes (6, 11) can cause benign hyperplasia such as condylomas and papillomas. Persistent infections with high-risk genotypes (16, 18, 31, 33, and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Interpretive report.
Results are reported as positive or negative for low-risk genotypes 6, 11, and/or high-risk genotypes 16, 18, 31, 33, and 51. If additional interpretation or analysis is needed, request PATCH / Pathology Consultation along with this test.
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATCH / Pathology Consultation along with this test.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88365 ​1 ​Human Papillomavirus Typing In Situ
​88364 ​1 ​Insitu Hybridization (FISH)
Synonyms/Keywords

HPV (Human Papillomavirus)

HPVHR

Papillomavirus

GPHPVHLISH
 
ISHPV611ISH
 
ISHPVAM16ISH
 
ISNEGHPVISH
 
ISDNAHPVISH
 
Test Components

Human papillomavirus (HPV) High-Risk E6/E7 RNA will be performed at the discretion of the Mayo pathologist.

Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Formalin-fixed, paraffin-embedded tissue block ​Entire block
​6 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue ​6 slides
Collection Processing
A pathology/diagnostic report and a brief history are required.
Acceptable Specimen Types
​10% neutral buffered formalin fixed tissue specimen/ paraffin embedded tissue block
Specimen Stability Information
Specimen Type Temperature Time
​Tissue Slides Ambient ​ Indefinite
​10% Neutral Buffered Formalin Fixed Tissue Specimen ​ Ambient (preferred) ​ 6 months
​Refrigerate ​1 month
Rejection Criteria
Wet/Frozen Tissue
​Cytology Smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Useful For
Detection of both low-risk (6, 11) and high-risk genotypes (16, 18, 31, 33, and 51) of human papillomavirus DNA in paraffin-embedded human tissue.
Test Components

Human papillomavirus (HPV) High-Risk E6/E7 RNA will be performed at the discretion of the Mayo pathologist.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Interpretive report.
Results are reported as positive or negative for low-risk genotypes 6, 11, and/or high-risk genotypes 16, 18, 31, 33, and 51. If additional interpretation or analysis is needed, request PATCH / Pathology Consultation along with this test.
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATCH / Pathology Consultation along with this test.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday - Friday ​5 days In Situ Hybridization (ISH)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88365 ​1 ​Human Papillomavirus Typing In Situ
​88364 ​1 ​Insitu Hybridization (FISH)
For most current information refer to the Marshfield Laboratory online reference manual.