Skip Ribbon Commands
Skip to main content
Sign In

23022 Vitamin D, 25-Hydroxy D2 and D3, Serum

Vitamin D, 25-Hydroxy D2 and D3, Serum
Test Code: VITD
Synonyms/Keywords
Calcidiol, 25-hydroxy  Vitamin D, 25-hydroxycholecalciferol, Vitamin D,  Vitamin D2 + D3, Total Vitamin D, 25-OH Vitamin D
Test Components
25-Hydroxyvitamin D2, 25-Hydroxyvitamin D3 and Total 25-Hydroxyvitamin D​
Useful For
​This is the first-line test for assessing Vitamin D status. Vitamin D is a fat-soluble vitamin that is involved in mineral homeostasis and plays an important role in calcium metabolism, parathyroid hormone release (PTH) and osteoblast formation. 25-hydroxyvitamin D levels are used to diagnose intestinal malabsorption, and vitamin D deficiency or intoxication. Additionally, 25-hydroxyvitamin D levels may be used to monitor therapeutic response in patients being treated for vitamin D-related disorders.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​
Green Top Tube (GTT) or (PST)
 
EDTA Lavender Top (LTT)
 
Serum Separator Tube (SST)​
 
Specimens in polypropylene aliquot tubes from Marshfield automation line are unacceptable and will be rejected from testing. 
0.5 mL Serum​ 0.5 mL Serum​ 0.3 mL Serum​
Collection Processing Instructions
Collect specimen in preferred/acceptable containers as listed above in "Specimen Requirements".  Separate serum/plasma, then aliquot into "Serum Transfer Tube" (available through Marshfield Labs) or a clean plastic tube.  Refrigerate sample.  However, serum/plasma left on cells is acceptable for 2 weeks refrigerated.
 
Further instructions for Marshfield specimen receiving/processing: Specimens aliquotted into polypropylene aliquot tubes from automation line are unacceptable and will be rejected from testing.  Do not aliquot into automation line aliquot tubes!
Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma​ Refrigerate​ 2 weeks​
Frozen 1 month​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 2-4 days​ UPLC MS/MS - Waters Acquity UPLC, XEVO TQ-S Tandem Mass Spectrometry System​
​Samples are run/analyzed overnight; results are available next business day. ​ ​ ​
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Reference Range Information
Performing Location Reference Range
Marshfield​

Specific reference ranges for 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 are not available

Reference range information is available for the total 25-hydroxyvitamin D and is as follows:

<12 ng/mL indicates severe deficiency with risk of rickets or osteomalacia

10 - 29 ng/mL mild to moderate deficiency with increased risk of osteoporosis

30 - 50 ng/mL optimum levels

51 - 80 ng/mL has increased risk of hypercalciuria

> 80 ng/mL toxicity possible.

Only the total 25-hydroxyvitamin D level should be considered when assessing 25-hydroxyvitamin D status.​

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82306​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Synonyms/Keywords
Calcidiol, 25-hydroxy  Vitamin D, 25-hydroxycholecalciferol, Vitamin D,  Vitamin D2 + D3, Total Vitamin D, 25-OH Vitamin D
Test Components
25-Hydroxyvitamin D2, 25-Hydroxyvitamin D3 and Total 25-Hydroxyvitamin D​
Ordering Applications
Ordering Application Description
​Centricity ​Vitamin D (25-Hydroxy) Total, D2, D3
​Cerner Vitamin D, 25-Hydroxy D2 and D3, Serum​
​COM ​Vitamin D (25-Hydroxy)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​
Green Top Tube (GTT) or (PST)
 
EDTA Lavender Top (LTT)
 
Serum Separator Tube (SST)​
 
Specimens in polypropylene aliquot tubes from Marshfield automation line are unacceptable and will be rejected from testing. 
0.5 mL Serum​ 0.5 mL Serum​ 0.3 mL Serum​
Collection Processing
Collect specimen in preferred/acceptable containers as listed above in "Specimen Requirements".  Separate serum/plasma, then aliquot into "Serum Transfer Tube" (available through Marshfield Labs) or a clean plastic tube.  Refrigerate sample.  However, serum/plasma left on cells is acceptable for 2 weeks refrigerated.
 
Further instructions for Marshfield specimen receiving/processing: Specimens aliquotted into polypropylene aliquot tubes from automation line are unacceptable and will be rejected from testing.  Do not aliquot into automation line aliquot tubes!
Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma​ Refrigerate​ 2 weeks​
Frozen 1 month​
Useful For
​This is the first-line test for assessing Vitamin D status. Vitamin D is a fat-soluble vitamin that is involved in mineral homeostasis and plays an important role in calcium metabolism, parathyroid hormone release (PTH) and osteoblast formation. 25-hydroxyvitamin D levels are used to diagnose intestinal malabsorption, and vitamin D deficiency or intoxication. Additionally, 25-hydroxyvitamin D levels may be used to monitor therapeutic response in patients being treated for vitamin D-related disorders.
Test Components
25-Hydroxyvitamin D2, 25-Hydroxyvitamin D3 and Total 25-Hydroxyvitamin D​
Reference Range Information
Performing Location Reference Range
Marshfield​

Specific reference ranges for 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 are not available

Reference range information is available for the total 25-hydroxyvitamin D and is as follows:

<12 ng/mL indicates severe deficiency with risk of rickets or osteomalacia

10 - 29 ng/mL mild to moderate deficiency with increased risk of osteoporosis

30 - 50 ng/mL optimum levels

51 - 80 ng/mL has increased risk of hypercalciuria

> 80 ng/mL toxicity possible.

Only the total 25-hydroxyvitamin D level should be considered when assessing 25-hydroxyvitamin D status.​

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 2-4 days​ UPLC MS/MS - Waters Acquity UPLC, XEVO TQ-S Tandem Mass Spectrometry System​
​Samples are run/analyzed overnight; results are available next business day. ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82306​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
For most current information refer to the Marshfield Laboratory online reference manual.