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23019 Vitamin B12

Vitamin B12
Test Code: B-12
Synonyms/Keywords
Vitamin B-12, B12, Cyanocobalamin​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 0.5 mL​ 0.4 mL​ 0.3 mL​
Collection Processing Instructions
​Refrigerate off the cells up to 4 hours. If analysis will be delayed longer than 4 hours, freeze the sample. Avoid repeated freezing and thawing of serum specimen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated off cells​ <4 hours​
Frozen​ >4 hours​
Rejection Criteria
Moderately hemolyzed
Interference
For testing performed at The Diagnostic & Treatment Center lab (Weston Center): High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday​​Less than 2 hours​ ​Homogeneous Sandwich Chemiluminescent Immunoassay/Siemens Dimension Vista
Marshfield​ Monday through Sunday​Less than 2 hours​ Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur ​
Reference Range Information
Performing Location Reference Range
All Performing Sites 160 - 1000 pg/mL​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82607 ​
Synonyms/Keywords
Vitamin B-12, B12, Cyanocobalamin​
Ordering Applications
Ordering Application Description
​Centricity

​a. B-12, Vitamin

b. Vit B-12

​Cerner ​Vitamin B-12
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 0.5 mL​ 0.4 mL​ 0.3 mL​
Collection Processing
​Refrigerate off the cells up to 4 hours. If analysis will be delayed longer than 4 hours, freeze the sample. Avoid repeated freezing and thawing of serum specimen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated off cells​ <4 hours​
Frozen​ >4 hours​
Rejection Criteria
Moderately hemolyzed
Interference
For testing performed at The Diagnostic & Treatment Center lab (Weston Center): High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Reference Range Information
Performing Location Reference Range
All Performing Sites 160 - 1000 pg/mL​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday​​Less than 2 hours​ ​Homogeneous Sandwich Chemiluminescent Immunoassay/Siemens Dimension Vista
Marshfield​ Monday through Sunday​Less than 2 hours​ Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82607 ​
For most current information refer to the Marshfield Laboratory online reference manual.