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23004 PSA, Free & Total

PSA, Free & Total
Test Code: PSA-F
Synonyms/Keywords
​Prostate Specific Antigen, Free & Total​
Test Components
Total Prostate Specific Antigen , Free Prostate Specific Antigen​
Specimen Requirements
 
 
 
 
 
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​1.0 mL​0.6 mL​0.5 mL​
Collection Processing Instructions
Remove serum from cells within 3 hours of centrifugation.​
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated​ 24 hours​
Frozen​ >24 hours to 5 months​
Rejection Criteria
Grossly hemolyzed
Interference
Human anti-mouse antibodies (HAMA) may be present in samples from patients who have received immunotherapy utilizing monoclonal antibodies.​
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ Less than 2 hours​ Hybritch Ab Total and Free PSA/Beckman Coulter DXI ​
Reference Range Information
Performing Location Reference Range
Marshfield​
Total PSA: 0.0-4.0 ng/mL
% Free PSA: = or > 25% ​
Interpretation
Includes measurement of Total PSA and Free PSA by the Hybritech methods, and calculation of Percent Free PSA as follows: %Free PSA = 100 x (Free/Total). Percent Free PSA is indicated primarily with Total PSA results in the 3-10 ng/mL range. Generally %Free PSA levels of 20% or more suggest a higher probability of BPH whereas levels of 10% or less reflect a higher probability of prostatic carcinoma (see table below). This test requires ICD9 codes and waivers. Please refer to the Marshfield Clinic Lab Order Sheet for further information. The following table indicates the Probability of Prostate Cancer for Total PSA's in the 4-10 range and various %Free PSA Levels for men with a non-suspect digital rectal exam: Probability of Prostate Cancer based on Age and %Free PSA
% FREE PSA     50-64 YEARS     65-75 YEARS
0.0 - 10.0%            56%                  55%
10.1 - 15.0%          24%                  35%
15.1 - 20.0%          17%                  23%
20.1 - 25.0%          10%                  20%
    >25.0%               5%                    9% ​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84154   ​ ​ ​1
 ​Free PSA
 ​

​84153
​

​1
​Total PSA​
​
Synonyms/Keywords
​Prostate Specific Antigen, Free & Total​
Test Components
Total Prostate Specific Antigen , Free Prostate Specific Antigen​
Ordering Applications
Ordering Application Description
​Centricity

​a. PSA, Total & Free

b. PSA-Total

​Cerner ​Prostate Specific Antigen Free
​COM ​PSA, Total & Free
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
 
 
 
 
 
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​1.0 mL​0.6 mL​0.5 mL​
Collection Processing
Remove serum from cells within 3 hours of centrifugation.​
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerated​ 24 hours​
Frozen​ >24 hours to 5 months​
Rejection Criteria
Grossly hemolyzed
Interference
Human anti-mouse antibodies (HAMA) may be present in samples from patients who have received immunotherapy utilizing monoclonal antibodies.​
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Test Components
Total Prostate Specific Antigen , Free Prostate Specific Antigen​
Reference Range Information
Performing Location Reference Range
Marshfield​
Total PSA: 0.0-4.0 ng/mL
% Free PSA: = or > 25% ​
Interpretation
Includes measurement of Total PSA and Free PSA by the Hybritech methods, and calculation of Percent Free PSA as follows: %Free PSA = 100 x (Free/Total). Percent Free PSA is indicated primarily with Total PSA results in the 3-10 ng/mL range. Generally %Free PSA levels of 20% or more suggest a higher probability of BPH whereas levels of 10% or less reflect a higher probability of prostatic carcinoma (see table below). This test requires ICD9 codes and waivers. Please refer to the Marshfield Clinic Lab Order Sheet for further information. The following table indicates the Probability of Prostate Cancer for Total PSA's in the 4-10 range and various %Free PSA Levels for men with a non-suspect digital rectal exam: Probability of Prostate Cancer based on Age and %Free PSA
% FREE PSA     50-64 YEARS     65-75 YEARS
0.0 - 10.0%            56%                  55%
10.1 - 15.0%          24%                  35%
15.1 - 20.0%          17%                  23%
20.1 - 25.0%          10%                  20%
    >25.0%               5%                    9% ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ Less than 2 hours​ Hybritch Ab Total and Free PSA/Beckman Coulter DXI ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84154   ​ ​ ​1
 ​Free PSA
 ​

​84153
​

​1
​Total PSA​
​
For most current information refer to the Marshfield Laboratory online reference manual.