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22983 Protein C Antigen, On Coumadin (500534)

Protein C Antigen, On Coumadin (500534)
Test Code: PROT-CM
Synonyms/Keywords
​Protein C Antigen, Protein C Antigen / Factor VII ratio
Test Components
​Test includes Protein C antigen, Factor VII antigen, and Prot C/Factor VII ratio.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
Pediatric Minimum Volume
Platelet poor plasma​ ​Citrated Blue Top Tube (BTT) 1 mL​ ​0.5 mL
Collection Processing Instructions

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a transfer tube. Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Specimen Stability Information
Specimen Type Temperature Time
Plasma

Freeze at -70 deg C.

(preferably at -70 deg. C, but -20 deg C is acceptable for outreach)

60 days
Rejection Criteria

Hemolysis; clotted specimen; specimen contaminated with heparin (ie, drawn with blood gases); samples containing fibrin clots; specimens received thawed for more than 24 hours.  

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
LabCorp forwards to Esoterix Tuesday, Friday 3-6 days ​ELISA
Reference Lab
Reference Range Information
Performing Location Reference Range
LabCorp​ 0.2:2.2 ratio​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​85302 ​1 ​Protein C Antigen
​85230​1​Factor VII
Synonyms/Keywords
​Protein C Antigen, Protein C Antigen / Factor VII ratio
Test Components
​Test includes Protein C antigen, Factor VII antigen, and Prot C/Factor VII ratio.
Ordering Applications
Ordering Application Description
​Centricity ​Prot C Ag-On Coumadin
​Cerner ​Protein C Antigen-ON Coumadin (300067)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
Pediatric Minimum Volume
Platelet poor plasma​ ​Citrated Blue Top Tube (BTT) 1 mL​ ​0.5 mL
Collection Processing

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a transfer tube. Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Specimen Stability Information
Specimen Type Temperature Time
Plasma

Freeze at -70 deg C.

(preferably at -70 deg. C, but -20 deg C is acceptable for outreach)

60 days
Rejection Criteria

Hemolysis; clotted specimen; specimen contaminated with heparin (ie, drawn with blood gases); samples containing fibrin clots; specimens received thawed for more than 24 hours.  

Test Components
​Test includes Protein C antigen, Factor VII antigen, and Prot C/Factor VII ratio.
Reference Range Information
Performing Location Reference Range
LabCorp​ 0.2:2.2 ratio​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
LabCorp forwards to Esoterix Tuesday, Friday 3-6 days ​ELISA
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​85302 ​1 ​Protein C Antigen
​85230​1​Factor VII
For most current information refer to the Marshfield Laboratory online reference manual.