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22793 Insulin Assay

Insulin Assay
Test Code: INSULIN
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 0.5 mL​ 0.3 mL​ 0.25 mL​
Collection Processing Instructions
Patient must be fasting a minimum of eight hours before obtaining specimen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ 8 hours​
Refrigerated​ 24 hours​
Frozen​ >24 hours​
Rejection Criteria
Grossly hemolyzed
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday 2 hours
 Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
Test Information
A glucose level should also be ordered on the same specimen. Patients on long-term insulin therapy may have anti-insulin antibodies which will falsely lower insulin results. Insulin assay measures exogenous as well as endogenous insulin; measurement of C-peptide may be helpful in differentiating the insulin source and in assessing the cause of hypoglycemic states.

Note: after 48 hours of refrigeration, insulin loses about 10% of its activity.​
Reference Range Information
Performing Location

Reference Range

Marshfield​

Fasting: 2.6 - 37.6 uU/mL

Reference range stated assumes a fasting sample

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83525 ​ ​ ​ ​ ​
Ordering Applications
Ordering Application Description
​Centricity ​Insulin
​Cerner ​Insulin Level Total
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 0.5 mL​ 0.3 mL​ 0.25 mL​
Collection Processing
Patient must be fasting a minimum of eight hours before obtaining specimen.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ 8 hours​
Refrigerated​ 24 hours​
Frozen​ >24 hours​
Rejection Criteria
Grossly hemolyzed
Reference Range Information
Performing Location

Reference Range

Marshfield​

Fasting: 2.6 - 37.6 uU/mL

Reference range stated assumes a fasting sample

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday 2 hours
 Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83525 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.