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22793 Insulin Assay

Insulin Assay
Test Code: INSULIN
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 1.0 mL​ 0.5 mL​ 0.3 mL​
Collection Processing Instructions
Patient must be fasting a minimum of eight hours before obtaining specimen.
RTT- physically separate plasma or serum from contact with cells as soon as possible or before transport.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ 8 hours​
Refrigerated​ 1 day​
Frozen​ >1 day​
Rejection Criteria
Trace hemolysis
Marked Icterus
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday
 2 hours
Immunoenzymatic assay/Beckman Coulter Unicel DXI ​
Test Information
A glucose level should also be ordered on the same specimen. Patients on long-term insulin therapy may have anti-insulin antibodies which will falsely lower insulin results. Insulin assay measures exogenous as well as endogenous insulin; measurement of C-peptide may be helpful in differentiating the insulin source and in assessing the cause of hypoglycemic states.

Note: after 48 hours of refrigeration, insulin loses about 10% of its activity.​
Reference Range Information
Performing Location

Reference Range

Marshfield​

1.9-23 uIU/mL​

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83525 ​ ​ ​ ​ ​
Ordering Applications
Ordering Application Description
​Centricity ​Insulin
​Cerner ​Insulin Level Total
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​ Serum​ Serum Separator Tube (SST) ​Red Top Tube (RTT)​ 1.0 mL​ 0.5 mL​ 0.3 mL​
Collection Processing
Patient must be fasting a minimum of eight hours before obtaining specimen.
RTT- physically separate plasma or serum from contact with cells as soon as possible or before transport.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ 8 hours​
Refrigerated​ 1 day​
Frozen​ >1 day​
Rejection Criteria
Trace hemolysis
Marked Icterus
Reference Range Information
Performing Location

Reference Range

Marshfield​

1.9-23 uIU/mL​

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday
 2 hours
Immunoenzymatic assay/Beckman Coulter Unicel DXI ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
83525 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.