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22752 HLA-B27

HLA-B27
Test Code: B27
Synonyms/Keywords
Flow Cytometry, B27, B27-SO​
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter’s syndrome, acute anterior uveitis, and inflammatory bowel disease.  HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​
Sodium-heparin Green Top Tube (GTT)
ACD Soln. A Yellow Top Tube​
1 mL​ 1 mL​ 0.5 mL​
Collection Processing Instructions
If the results are indeterminate, a confirmatory test is needed. Current sample may be used for confirmatory testing if requested within 10 days of draw date.  Order as a MISC and add comment B27-SO. Call Flow Cytometry (800-222-5835 ext 16385) for more information.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 5 days ​
Rejection Criteria
Refrigerated or frozen
Fixed specimens​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 4 days​ Flow Cytometry​
Test Information

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86812​
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

Synonyms/Keywords
Flow Cytometry, B27, B27-SO​
Ordering Applications
Ordering Application Description
​Centricity ​HLA B27
​Cerner ​HLA B27
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)​
Sodium-heparin Green Top Tube (GTT)
ACD Soln. A Yellow Top Tube​
1 mL​ 1 mL​ 0.5 mL​
Collection Processing
If the results are indeterminate, a confirmatory test is needed. Current sample may be used for confirmatory testing if requested within 10 days of draw date.  Order as a MISC and add comment B27-SO. Call Flow Cytometry (800-222-5835 ext 16385) for more information.​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Room temperature​ 5 days ​
Rejection Criteria
Refrigerated or frozen
Fixed specimens​
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter’s syndrome, acute anterior uveitis, and inflammatory bowel disease.  HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 4 days​ Flow Cytometry​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86812​
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

For most current information refer to the Marshfield Laboratory online reference manual.