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22732 RH-Immune Globulin

RH-Immune Globulin
Test Code: RHIG
Synonyms/Keywords
RHo-Gam, RhIg​
Test Components
ABO/RH, ABSC, FBSC​
Useful For
Prevention of sensitization and the development of Anti-D by the D(Rh) negative patient  ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​		 Two 3 mL EDTA Lavender Top Tubes (LTT) ​ 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Collection Processing Instructions
28 Weeks Gestation, First or Second Trimester (bleed, abortion, miscarriages, ectopic pregnancy): ABSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
 
Third Trimester (abortion, miscarriage, ectopic pregnancy, vaginal bleed, fetal death): ABSC, FBSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
 
Postpartum: FBSC (drawn > 1 hour after delivery)
ABO/RH and ABSC if no current record of these tests in Transfusion Service computer system​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Refrigerate​ Perform testing within 48 hours of collection​
Rejection Criteria
Serum separator tubes (SST)
Frozen samples​
Hemolyzed samples​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Serological​
Test Information
RhIg is a highly concentrated solution of IgG anti-D derived from pooled human plasma and contains about 300mg of anti-D IgG globulin. The administration of RhIg to a D(Rh) negative patient will prevent sensitization and the development of Anti-D by the patient. Send a separate Blood Bank request form for "Rh Immune Globulin".
If FBSC (Fetal Bleed Screen) is positive, the physician must be notified that a Kleihauer-Betke test is required. The Kleihauer-Betke test will determine the number of vials of RhIg needed.
Refer to Fetal Bleed Screen Test procedure​
Reference Range Information
Performing Location Reference Range
Marshfield​ No reference ranges are available​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86999  ​ ​ ​ ​ ​
Synonyms/Keywords
RHo-Gam, RhIg​
Test Components
ABO/RH, ABSC, FBSC​
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​		 Two 3 mL EDTA Lavender Top Tubes (LTT) ​ 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Collection Processing
28 Weeks Gestation, First or Second Trimester (bleed, abortion, miscarriages, ectopic pregnancy): ABSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
 
Third Trimester (abortion, miscarriage, ectopic pregnancy, vaginal bleed, fetal death): ABSC, FBSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
 
Postpartum: FBSC (drawn > 1 hour after delivery)
ABO/RH and ABSC if no current record of these tests in Transfusion Service computer system​
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ Refrigerate​ Perform testing within 48 hours of collection​
Rejection Criteria
Serum separator tubes (SST)
Frozen samples​
Hemolyzed samples​
Useful For
Prevention of sensitization and the development of Anti-D by the D(Rh) negative patient  ​
Test Components
ABO/RH, ABSC, FBSC​
Reference Range Information
Performing Location Reference Range
Marshfield​ No reference ranges are available​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Serological​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86999  ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.