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22713 Hepatitis B Virus PCR, Quant, NAM

Hepatitis B Virus PCR, Quant, NAM
Test Code: HBVQT
Synonyms/Keywords
​HBV Viral Load, HBV NAM, Quantitative, Hep B RNA Quant, HBV RNA
Useful For

​The test is intended for use as an aid in the clinical management of patients with chronic HBV infection.  The results from this test must be interpreted within the context of all relevant clinical and laboratory findings.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Serum Separator Tube (SST) ​ ​1.5 mL 1.5 mL​ 1.0 mL​
Collection Processing Instructions

Whole blood may be stored at 2 to 25 degrees C for up to 24 hours prior to centrifugation.

In order to ensure sample integrity, testing will not be performed as add-on requests. Direct aliquots from primary tubes are required

Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​Refrigerate ​< 5 days
Frozen @ -70 degrees Celsius​ >= 5 days
Rejection Criteria
Specimens other than Serum
​Hemolyzed samples
​More than two freeze-thaw cycles
​Previously opened tubes
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​ Friday
6-8 hours*
 Nucleic Acid Amplification (NAM)  ​
​ ​*Testing performed once a week.​ ​
Sample must be re​ceived in the lab by 0700 on the testing Friday to be tested same day.​  ​ ​
Test Information
​The Panther Aptima quantitative HBV test is an FDA approved in vitro nucleic acid amplification test. It is intended for use as an aid in the management of patients with chronic HBV infections undergoing HBV antiviral drug therapy.
This assay quantitates HBV genotypes A, B, C, D, E, F, G, and H over the range of 10 IU/mL to 1 billion IU/mL.
Reference Range Information
Performing Location Reference Range
​Marshfield ​No HBV RNA detected
Interpretation
Positives are reported as 10 to 1,000,000,000 IU/mL. Qualitative detection of HBVRNA below 1 IU/mL is reported as <10 IU/mL with the following interpretive comment: HBVRNA is detected but is below the lower limit of quantitation (10 IU/mL).
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87517 ​ ​ ​ ​
Synonyms/Keywords
​HBV Viral Load, HBV NAM, Quantitative, Hep B RNA Quant, HBV RNA
Ordering Applications
Ordering Application Description
​Centricity ​Hepatitis B Virus PCR, Quant
​Cerner ​Hepatitis B Virus PCR, Quant
​COM ​Hepatitis B Virus PCR, Quant
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Serum Separator Tube (SST) ​ ​1.5 mL 1.5 mL​ 1.0 mL​
Collection Processing

Whole blood may be stored at 2 to 25 degrees C for up to 24 hours prior to centrifugation.

In order to ensure sample integrity, testing will not be performed as add-on requests. Direct aliquots from primary tubes are required

Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​Refrigerate ​< 5 days
Frozen @ -70 degrees Celsius​ >= 5 days
Rejection Criteria
Specimens other than Serum
​Hemolyzed samples
​More than two freeze-thaw cycles
​Previously opened tubes
Useful For

​The test is intended for use as an aid in the clinical management of patients with chronic HBV infection.  The results from this test must be interpreted within the context of all relevant clinical and laboratory findings.

Reference Range Information
Performing Location Reference Range
​Marshfield ​No HBV RNA detected
Interpretation
Positives are reported as 10 to 1,000,000,000 IU/mL. Qualitative detection of HBVRNA below 1 IU/mL is reported as <10 IU/mL with the following interpretive comment: HBVRNA is detected but is below the lower limit of quantitation (10 IU/mL).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​ Friday
6-8 hours*
 Nucleic Acid Amplification (NAM)  ​
​ ​*Testing performed once a week.​ ​
Sample must be re​ceived in the lab by 0700 on the testing Friday to be tested same day.​  ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87517 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.