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22707 Hepatitis A Antibody, Total

Hepatitis A Antibody, Total
Test Code: A
Synonyms/Keywords
Anti-HAV, HAVAB, Hep A Virus Antibody​
Useful For
In aiding in the diagnosis of previous or ongoing hepatitis A viral infection. Also useful for the determination of susceptibility to Hepatitis A.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST)​ Red Top Tube (RTT)​ 1 mL​ 0.5 mL​ 0.3 mL​
Collection Processing Instructions
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube. Specimen must be free of particulate matter including fibrin.
 
Regional/Outreach sites:  Please send samples to Marshfield frozen.​
  
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​​ Ambient​ <8 hours​
Refrigerated​ <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
Grossly icteric​
​Specimens containing precipitate
Cadaver specimens​
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay. Patients receiving therapy with high doses of biotin (>5 mg/day), should be tested 8 hours after last biotin administration.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​
Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur
Test Information

Hepatitis A virus (HAV) is endemic throughout the world, however, occurring most commonly in areas of poor hygiene and low socioeconomic conditions. The virus is transmitted primarily by the fecal-oral route, and it is spread by close person-to-person contact and by food- and water-borne epidemics. Viral spread by parenteral routes (e.g., exposure to blood) is possible but rare, because infected individuals are viremic for a short period of time (usually <3 weeks). There is little or no evidence of transplacental transmission from mother to fetus or transmission to newborn during delivery.

Limitations
• The results determined by different assays from different manufacturers can vary due to differences in assay specificities and cannot be used interchangeably.
• A nonreactive test result does not exclude the possibility of exposure to hepatitis A virus.
• HAV Total assay does not distinguish among different classes of antibodies. The assay cannot be used to determine if a reactive sample is due to an acute infection or is the result of a previous infection.
• Assay performance characteristics have not been evaluated for immunocompromised, immunosuppressed, cord blood and neonatal patients.
• A reactive or positive result does not exclude co-infection by another hepatitis virus.

Reference Range Information
Performing Location Reference Range
Marshfield​ Reported as "Reactive" or "Non-reactive".​
Interpretation

Antibodies to HAV (anti-HAV) are detectable by the time symptoms occur, usually 15 to 45 days after exposure. The early antibody response is largely comprised of the IgM antibody subclass. Anti-HAV IgM is detectable for 3 to 6 months after the onset of illness, whereas anti-HAV IgG can persist indefinitely. The specific determination of anti-HAV IgM is the most useful serological marker for diagnosing acute HAV infection. Total anti-HAV is used primarily for determination of previous exposure to Hepatitis A virus. The measurement of anti-HAV total activity is also used to identify HAV susceptible individuals for vaccination. Positive results should be correlated with the patient’s clinical history. A positive anti-HAV total result with a negative anti-HAV IgM result indicates probable immunity to hepatitis A from either natural infection or vaccination. However, this assay does not have FDA clearance for confirmation of immunity, whether by natural infection or vaccination.

Acute hepatitis A is a reportable disease in Wisconsin and other states.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86708   ​ ​ ​ ​ ​
Synonyms/Keywords
Anti-HAV, HAVAB, Hep A Virus Antibody​
Ordering Applications
Ordering Application Description
Centricity

​Hepatitis A IgG

and

Hep. A Virus Ab (HAVAB)

​Cerner ​Hepatitis A Antibody, Total
​Clinical Order Manager ​Hep A Virus Antibody
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST)​ Red Top Tube (RTT)​ 1 mL​ 0.5 mL​ 0.3 mL​
Collection Processing
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube. Specimen must be free of particulate matter including fibrin.
 
Regional/Outreach sites:  Please send samples to Marshfield frozen.​
  
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​​ Ambient​ <8 hours​
Refrigerated​ <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
Grossly icteric​
​Specimens containing precipitate
Cadaver specimens​
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay. Patients receiving therapy with high doses of biotin (>5 mg/day), should be tested 8 hours after last biotin administration.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Useful For
In aiding in the diagnosis of previous or ongoing hepatitis A viral infection. Also useful for the determination of susceptibility to Hepatitis A.
Reference Range Information
Performing Location Reference Range
Marshfield​ Reported as "Reactive" or "Non-reactive".​
Interpretation

Antibodies to HAV (anti-HAV) are detectable by the time symptoms occur, usually 15 to 45 days after exposure. The early antibody response is largely comprised of the IgM antibody subclass. Anti-HAV IgM is detectable for 3 to 6 months after the onset of illness, whereas anti-HAV IgG can persist indefinitely. The specific determination of anti-HAV IgM is the most useful serological marker for diagnosing acute HAV infection. Total anti-HAV is used primarily for determination of previous exposure to Hepatitis A virus. The measurement of anti-HAV total activity is also used to identify HAV susceptible individuals for vaccination. Positive results should be correlated with the patient’s clinical history. A positive anti-HAV total result with a negative anti-HAV IgM result indicates probable immunity to hepatitis A from either natural infection or vaccination. However, this assay does not have FDA clearance for confirmation of immunity, whether by natural infection or vaccination.

Acute hepatitis A is a reportable disease in Wisconsin and other states.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​
Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86708   ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.